BacMap

Definition	Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome.
Accession	NC_011369
Length	4,537,948

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The map label for this gene is cysA [C]

Identifier: 209548189

GI number: 209548189

Start: 607775

End: 608449

Strand: Reverse

Name: cysA [C]

Synonym: Rleg2_0583

Alternate gene names: 209548189

Gene position: 608449-607775 (Counterclockwise)

Preceding gene: 209548191

Following gene: 209548188

Centisome position: 13.41

GC content: 66.07

Gene sequence:

>675_bases
TTGGACATAGAATTCGAAGGCGCCGGGGTCAGTTTCGGGGCGCGGGTGGCGCTGGAGCCGCTGACGCTTGGCATCAGCGG
CAGACGCATCGGCGTCATCGGGCTGAACGGCTCGGGCAAGACCACTTTCGCGCGGCTGATCAATGGGCTGACCAAGCCGA
CGGTCGGGCGGGTCACCGTTAACGGCCGCGACACGGCCGACGAAAAGACAGCCGCAGCCGATGTCGGCTTCATTTTTCAG
TCGCCACAGAACCAGATCATCATGCCGATCGTCCGGGACGACATCGCCTTCGGGCTGAAGCGGCGCGGCTTCACCAAGGC
GGAGATCGAGGCGAAGGTCGAGGGCGTGCTGGCCCGCTTCGGCGCCGAGGCGCTGGCCGGGCGGCGGGCGCACGAGCTTT
CCGGCGGCGAGCTGCAGGTGGCAGCCCTCTGCTCGGTGCTGGCGACGGGGCCTGGCATTCTCATCCTCGACGAACCCACC
AACCAACTCGATCTCAAGAACCGGGCGCTGGTCGAAGGAATTATCGCCGGGCTTGCCGAAAGCGCCATCGTCATCACCCA
TGATCTGGAGCTGATCGCCGGTTTCGAGCGGGTGCTGCTGTTTCACGAAGGGCGGCTTGCCGCGGATGCGCCGGCGACTG
AGGCGATCGCCCGCTACAGGGAGATCGCCCTCTGA

Upstream 100 bases:

>100_bases
CGGCATATCCCTTCGCCCCGTTTACGGGGAGAAGGTGCCGGCAGGCGGATGAGGGGCCGGCATCGGCAATCTCCTCCGAT
GAAAGATGCGAGGGACTGGT

Downstream 100 bases:

>100_bases
TGCAGTCGCTCTATGTCGAAGGCAACAGCATCATGCATCGGCTTTCGCCGCGGGTGAAGCTGCTGTCGCTTGCGATCTTC
AGCATCGTGCTGTTCATCAC

Product: ABC transporter

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDIEFEGAGVSFGARVALEPLTLGISGRRIGVIGLNGSGKTTFARLINGLTKPTVGRVTVNGRDTADEKTAAADVGFIFQ
SPQNQIIMPIVRDDIAFGLKRRGFTKAEIEAKVEGVLARFGAEALAGRRAHELSGGELQVAALCSVLATGPGILILDEPT
NQLDLKNRALVEGIIAGLAESAIVITHDLELIAGFERVLLFHEGRLAADAPATEAIARYREIAL

Sequences:

>Translated_224_residues
MDIEFEGAGVSFGARVALEPLTLGISGRRIGVIGLNGSGKTTFARLINGLTKPTVGRVTVNGRDTADEKTAAADVGFIFQ
SPQNQIIMPIVRDDIAFGLKRRGFTKAEIEAKVEGVLARFGAEALAGRRAHELSGGELQVAALCSVLATGPGILILDEPT
NQLDLKNRALVEGIIAGLAESAIVITHDLELIAGFERVLLFHEGRLAADAPATEAIARYREIAL
>Mature_224_residues
MDIEFEGAGVSFGARVALEPLTLGISGRRIGVIGLNGSGKTTFARLINGLTKPTVGRVTVNGRDTADEKTAAADVGFIFQ
SPQNQIIMPIVRDDIAFGLKRRGFTKAEIEAKVEGVLARFGAEALAGRRAHELSGGELQVAALCSVLATGPGILILDEPT
NQLDLKNRALVEGIIAGLAESAIVITHDLELIAGFERVLLFHEGRLAADAPATEAIARYREIAL

Specific function: Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system [H]

COG id: COG1122

COG function: function code P; ABC-type cobalt transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI9955963, Length=212, Percent_Identity=27.3584905660377, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI42741659, Length=224, Percent_Identity=30.3571428571429, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI255708477, Length=231, Percent_Identity=27.7056277056277, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI156105685, Length=220, Percent_Identity=30.4545454545455, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI9961252, Length=226, Percent_Identity=29.2035398230088, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4505771, Length=226, Percent_Identity=29.2035398230088, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI9961250, Length=226, Percent_Identity=29.2035398230088, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI148612844, Length=237, Percent_Identity=26.5822784810127, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI150417984, Length=181, Percent_Identity=30.939226519337, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI9665248, Length=220, Percent_Identity=26.3636363636364, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI9955966, Length=229, Percent_Identity=23.5807860262009, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI45243524, Length=229, Percent_Identity=23.5807860262009, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI9955968, Length=229, Percent_Identity=23.5807860262009, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27436953, Length=188, Percent_Identity=25, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1788761, Length=222, Percent_Identity=36.036036036036, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI87081791, Length=195, Percent_Identity=31.7948717948718, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87081709, Length=212, Percent_Identity=29.7169811320755, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1787029, Length=221, Percent_Identity=32.579185520362, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787712, Length=220, Percent_Identity=30, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787370, Length=220, Percent_Identity=29.0909090909091, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI87081834, Length=195, Percent_Identity=31.7948717948718, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI87082267, Length=200, Percent_Identity=29, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1787089, Length=239, Percent_Identity=28.8702928870293, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1786563, Length=186, Percent_Identity=31.7204301075269, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789032, Length=199, Percent_Identity=34.1708542713568, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1787164, Length=208, Percent_Identity=32.2115384615385, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787500, Length=193, Percent_Identity=32.1243523316062, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994943, Length=219, Percent_Identity=32.4200913242009, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI2367384, Length=180, Percent_Identity=31.6666666666667, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI1789891, Length=203, Percent_Identity=31.0344827586207, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1789873, Length=218, Percent_Identity=29.8165137614679, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790467, Length=213, Percent_Identity=28.169014084507, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788225, Length=221, Percent_Identity=28.0542986425339, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786872, Length=194, Percent_Identity=32.4742268041237, Blast_Score=74, Evalue=5e-15,
Organism=Escherichia coli, GI1786345, Length=211, Percent_Identity=29.8578199052133, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1789672, Length=229, Percent_Identity=27.5109170305677, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786703, Length=199, Percent_Identity=30.6532663316583, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788165, Length=215, Percent_Identity=27.906976744186, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787105, Length=208, Percent_Identity=28.3653846153846, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1786398, Length=186, Percent_Identity=31.7204301075269, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788450, Length=200, Percent_Identity=28.5, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1790739, Length=231, Percent_Identity=26.4069264069264, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1786253, Length=196, Percent_Identity=28.0612244897959, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788644, Length=199, Percent_Identity=30.6532663316583, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1790162, Length=206, Percent_Identity=25.2427184466019, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786654, Length=233, Percent_Identity=26.1802575107296, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789962, Length=195, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI48994883, Length=200, Percent_Identity=30.5, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787758, Length=189, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI48995001, Length=222, Percent_Identity=29.2792792792793, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787547, Length=213, Percent_Identity=24.4131455399061, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533592, Length=241, Percent_Identity=28.2157676348548, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193208177, Length=234, Percent_Identity=29.0598290598291, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193209708, Length=234, Percent_Identity=29.4871794871795, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71988343, Length=221, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17565938, Length=218, Percent_Identity=25.6880733944954, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558664, Length=203, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI212646699, Length=202, Percent_Identity=27.2277227722772, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17543740, Length=215, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17533971, Length=226, Percent_Identity=27.4336283185841, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71984940, Length=215, Percent_Identity=25.5813953488372, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=204, Percent_Identity=24.0196078431373, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=236, Percent_Identity=25.8474576271186, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71996809, Length=231, Percent_Identity=26.4069264069264, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193211015, Length=231, Percent_Identity=26.4069264069264, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI193211017, Length=231, Percent_Identity=26.4069264069264, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=161, Percent_Identity=29.8136645962733, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996550, Length=218, Percent_Identity=28.8990825688073, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17569145, Length=203, Percent_Identity=25.615763546798, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71992285, Length=207, Percent_Identity=24.6376811594203, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=245, Percent_Identity=27.7551020408163, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324985, Length=222, Percent_Identity=27.9279279279279, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136662, Length=220, Percent_Identity=25.4545454545455, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281363262, Length=231, Percent_Identity=26.4069264069264, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653245, Length=231, Percent_Identity=26.4069264069264, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI23397593, Length=202, Percent_Identity=28.7128712871287, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571209, Length=211, Percent_Identity=23.696682464455, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648314, Length=166, Percent_Identity=29.5180722891566, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24666092, Length=212, Percent_Identity=26.4150943396226, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI221512771, Length=205, Percent_Identity=29.7560975609756, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24641565, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI85726357, Length=227, Percent_Identity=26.431718061674, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357907, Length=233, Percent_Identity=24.0343347639485, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24659289, Length=232, Percent_Identity=23.7068965517241, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 23653; Mature: 23653

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEFEGAGVSFGARVALEPLTLGISGRRIGVIGLNGSGKTTFARLINGLTKPTVGRVTV
CCEEECCCCCCCCCEEEEEEEEEEECCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEEE
NGRDTADEKTAAADVGFIFQSPQNQIIMPIVRDDIAFGLKRRGFTKAEIEAKVEGVLARF
CCCCCCCCHHHHHHEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GAEALAGRRAHELSGGELQVAALCSVLATGPGILILDEPTNQLDLKNRALVEGIIAGLAE
CHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCC
SAIVITHDLELIAGFERVLLFHEGRLAADAPATEAIARYREIAL
CEEEEEECHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MDIEFEGAGVSFGARVALEPLTLGISGRRIGVIGLNGSGKTTFARLINGLTKPTVGRVTV
CCEEECCCCCCCCCEEEEEEEEEEECCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEEE
NGRDTADEKTAAADVGFIFQSPQNQIIMPIVRDDIAFGLKRRGFTKAEIEAKVEGVLARF
CCCCCCCCHHHHHHEEEEEECCCCCEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GAEALAGRRAHELSGGELQVAALCSVLATGPGILILDEPTNQLDLKNRALVEGIIAGLAE
CHHHHHCCCHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCC
SAIVITHDLELIAGFERVLLFHEGRLAADAPATEAIARYREIAL
CEEEEEECHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA