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The map label for this gene is ispE

Identifier: 209548127

GI number: 209548127

Start: 534806

End: 535702

Strand: Reverse

Name: ispE

Synonym: Rleg2_0521

Alternate gene names: 209548127

Gene position: 535702-534806 (Counterclockwise)

Preceding gene: 209548128

Following gene: 209548126

Centisome position: 11.8

GC content: 67.22

Gene sequence:

>897_bases
ATGCCTGAAGCGGGCCTGGCCGAGGCTTTCGGCGTCACCGAAGAGGCGCGCGCAAAGATCAATCTCGCTTTGCATGTGAC
AGGCCAGCGGGCAGATGGCTATCATCTGCTCGACATGCTGGTGACCTTTGCCGATTGCGGCGACCGGCTGGGCTTCCTGC
CTGCCCAGACCGACGCCTTCACCCTGTCGGGTCGCTTCGGCGAGATGCTGGCCGGCGACGGCGGCACCAATCTGGTGCTG
CGGGCGCGCGATCTCCTGCGCGAGCAGTTCGGCGCCCTCGCCTTCCCCGTCCATATCCACCTGCAAAAGAACCTGCCTGT
TGCCTCCGGCATCGGCGGCGGCTCGGCCGATGCGGCCGCGGCGCTGCGCGGGCTGATGCGGCTCTGGGGCATGAGCCTGC
CGGTGGAGGCGCTTGCCAGTCTGGCGCTGAAGCTCGGCGCCGACGTGCCGATGTGCCTTGAAAGCCGGCCGCTAATTGCC
CGCGGTATCGGCGAGGAGATCGAGGCGGTGCCGGATCTGCCGGCCTTTGCCATGGTGCTCGCCAATCCGCTGAAGGGTGT
GTCGACGCCTGAGGTGTTCCGCCGGCTGACGACAAAGAACAATTCGGCCCTGAGCCTCGCACCCGGTCTGTCCGGGAGTG
CCGGCTGGCTGGCAGTAATCGATGCCGCCCGCAATGACCTGGAACCGCCGGCGCGTCAGCTGGTGCCCGAGATTGCGGTG
ATCTCGGCGATGCTGCAGGCCCGCGGCGCGCTTTTGACGCGGATGTCCGGCTCCGGCGCTACCTGTTTCGGGATCTTTGC
GAGCATGGCTGAGGCGCAAGACGCGGCGGCAGCCCTTCACGGCGAGCGGCCCGACTGGTATTTCCAGGCGACGGAAACGG
TTTCGGGAGGCATGTGA

Upstream 100 bases:

>100_bases
CGCCGTCAGCGACGATGCCAAGGCGGCCGACAAGAAGCAGCCGGATCCGGCCCCGGTCACGCCGCCGCCGGTCGACAAGA
AATCCTGACGGACGACAAAC

Downstream 100 bases:

>100_bases
TGGCAGATCAGGACGACAACCGCCCCTTCATCGCCGTCGGCATTGCGGTTCTCACCGTCTCGGATACGCGCACGCCGGAA
ACGGACAAGTCAGGCGACAC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPEAGLAEAFGVTEEARAKINLALHVTGQRADGYHLLDMLVTFADCGDRLGFLPAQTDAFTLSGRFGEMLAGDGGTNLVL
RARDLLREQFGALAFPVHIHLQKNLPVASGIGGGSADAAAALRGLMRLWGMSLPVEALASLALKLGADVPMCLESRPLIA
RGIGEEIEAVPDLPAFAMVLANPLKGVSTPEVFRRLTTKNNSALSLAPGLSGSAGWLAVIDAARNDLEPPARQLVPEIAV
ISAMLQARGALLTRMSGSGATCFGIFASMAEAQDAAAALHGERPDWYFQATETVSGGM

Sequences:

>Translated_298_residues
MPEAGLAEAFGVTEEARAKINLALHVTGQRADGYHLLDMLVTFADCGDRLGFLPAQTDAFTLSGRFGEMLAGDGGTNLVL
RARDLLREQFGALAFPVHIHLQKNLPVASGIGGGSADAAAALRGLMRLWGMSLPVEALASLALKLGADVPMCLESRPLIA
RGIGEEIEAVPDLPAFAMVLANPLKGVSTPEVFRRLTTKNNSALSLAPGLSGSAGWLAVIDAARNDLEPPARQLVPEIAV
ISAMLQARGALLTRMSGSGATCFGIFASMAEAQDAAAALHGERPDWYFQATETVSGGM
>Mature_297_residues
PEAGLAEAFGVTEEARAKINLALHVTGQRADGYHLLDMLVTFADCGDRLGFLPAQTDAFTLSGRFGEMLAGDGGTNLVLR
ARDLLREQFGALAFPVHIHLQKNLPVASGIGGGSADAAAALRGLMRLWGMSLPVEALASLALKLGADVPMCLESRPLIAR
GIGEEIEAVPDLPAFAMVLANPLKGVSTPEVFRRLTTKNNSALSLAPGLSGSAGWLAVIDAARNDLEPPARQLVPEIAVI
SAMLQARGALLTRMSGSGATCFGIFASMAEAQDAAAALHGERPDWYFQATETVSGGM

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=281, Percent_Identity=33.0960854092527, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_RHILW (B5ZS25)

Other databases:

- EMBL:   CP001191
- RefSeq:   YP_002280044.1
- ProteinModelPortal:   B5ZS25
- SMR:   B5ZS25
- GeneID:   6979237
- GenomeReviews:   CP001191_GR
- KEGG:   rlt:Rleg2_0521
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK14608
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31117; Mature: 30986

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: ACT_SITE 19-19 ACT_SITE 148-148

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAGLAEAFGVTEEARAKINLALHVTGQRADGYHLLDMLVTFADCGDRLGFLPAQTDAF
CCCCCCHHHHCCCHHHHCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEE
TLSGRFGEMLAGDGGTNLVLRARDLLREQFGALAFPVHIHLQKNLPVASGIGGGSADAAA
EECCCHHHHHCCCCCCEEEHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCHHHHH
ALRGLMRLWGMSLPVEALASLALKLGADVPMCLESRPLIARGIGEEIEAVPDLPAFAMVL
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHCCCCHHHHHHH
ANPLKGVSTPEVFRRLTTKNNSALSLAPGLSGSAGWLAVIDAARNDLEPPARQLVPEIAV
HHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCHHEEEEHHHCCCCCCHHHHHHHHHH
ISAMLQARGALLTRMSGSGATCFGIFASMAEAQDAAAALHGERPDWYFQATETVSGGM
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
PEAGLAEAFGVTEEARAKINLALHVTGQRADGYHLLDMLVTFADCGDRLGFLPAQTDAF
CCCCCHHHHCCCHHHHCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEE
TLSGRFGEMLAGDGGTNLVLRARDLLREQFGALAFPVHIHLQKNLPVASGIGGGSADAAA
EECCCHHHHHCCCCCCEEEHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCCHHHHH
ALRGLMRLWGMSLPVEALASLALKLGADVPMCLESRPLIARGIGEEIEAVPDLPAFAMVL
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHCCCCHHHHCCCHHHHHCCCCHHHHHHH
ANPLKGVSTPEVFRRLTTKNNSALSLAPGLSGSAGWLAVIDAARNDLEPPARQLVPEIAV
HHHCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCHHEEEEHHHCCCCCCHHHHHHHHHH
ISAMLQARGALLTRMSGSGATCFGIFASMAEAQDAAAALHGERPDWYFQATETVSGGM
HHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA