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The map label for this gene is trpI [H]

Identifier: 209548052

GI number: 209548052

Start: 455955

End: 456833

Strand: Direct

Name: trpI [H]

Synonym: Rleg2_0445

Alternate gene names: 209548052

Gene position: 455955-456833 (Clockwise)

Preceding gene: 209548046

Following gene: 209548053

Centisome position: 10.05

GC content: 67.92

Gene sequence:

>879_bases
ATGCGTCTTCCACCGCTCAATGCCGTCCGCGCCTTCGAGGCGGTCTGCCGCCACGGCAGCATCCTGAAGGCCGCCGAAGA
GCTGAAGGTGGTGCGCGGCGCCGTGCGACAGCAGATCGCCACGCTGGAGAGCCATTTCGGCCGCAAGCTCTTTGTGCGCG
ATGGCCGCAGGCTGATCCCGACCGCAGAGGCGACCGCTTTCGCCGAAGCATCGGGCGCGGCTTTCGACATTCTGCAACGC
GCCGCTTGCGAGCTCTCGGGCACCGGGCCGGGCCGCATCCGGCTCGGCGTACCCTCCGCTTTCGCGGTCTGGTGGCTGAT
GCCGCGTGTTGCCGACATGCAGGCAAGCCTCGGCGATATGGCGGTCGATATCGTGCCGATGAGCGTCGTCGAACCGTTGC
TGATGCACCCGGAACTGGACGCGGTGATCATGGGCGGCGAATACCGGCCGGCCGCCGGCGTGACCGCTGTGCGTTTCATG
GAGGATGAGTTCGGTCCGGTCGCAACGCCGGCGCTTGCAGCGACGCTCTCGGAGAGCCCGGCGGCGATGGGCCGACTCAC
CATGCTTGCGAGCCGCAGCGTTCCGAAACTCTGGGACGAATGGTTTGCCGAAAGCGGCACGCCGCCGGTCGCCTTTTCCC
GCGTCCAAGAATTCGAGGATCTGCTGCTGGCGCTCGGTGCCGCCCGCTCCGGGCTCGGCATCGCCCTTGCGCCGCGCGCC
TCGATCGAGGACGATCTTCAGCGCGGGCAGCTGGTCGCGCCCTATGGTTTCATCGCCCGTCCGTCCGGCTACAGCCTCTG
CTGCCGCACGTCGGATGCGAAACGTCCGGATTTCTCAGCTTTGTCCGGCTGGCTGCGCCGGTGCGGGAGGCCGAGCTGA

Upstream 100 bases:

>100_bases
CGAATGCGTCACATTGCACAGGTGTTCAATATGGCGCGGTCTTGCCTTTCCTTCAATGGCAAGATCAAGTGATCCACTGT
CATTTCTGATCGTGGTCATC

Downstream 100 bases:

>100_bases
AAGGGCAGATTTTCTGCCCTTTCGCCAGAATAACCTCCATATTCGCCGCCCTGTCGCTTCGCTAAAGATTGTTCGAGTAT
CGGCAACAGGGATGAAGTGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRLPPLNAVRAFEAVCRHGSILKAAEELKVVRGAVRQQIATLESHFGRKLFVRDGRRLIPTAEATAFAEASGAAFDILQR
AACELSGTGPGRIRLGVPSAFAVWWLMPRVADMQASLGDMAVDIVPMSVVEPLLMHPELDAVIMGGEYRPAAGVTAVRFM
EDEFGPVATPALAATLSESPAAMGRLTMLASRSVPKLWDEWFAESGTPPVAFSRVQEFEDLLLALGAARSGLGIALAPRA
SIEDDLQRGQLVAPYGFIARPSGYSLCCRTSDAKRPDFSALSGWLRRCGRPS

Sequences:

>Translated_292_residues
MRLPPLNAVRAFEAVCRHGSILKAAEELKVVRGAVRQQIATLESHFGRKLFVRDGRRLIPTAEATAFAEASGAAFDILQR
AACELSGTGPGRIRLGVPSAFAVWWLMPRVADMQASLGDMAVDIVPMSVVEPLLMHPELDAVIMGGEYRPAAGVTAVRFM
EDEFGPVATPALAATLSESPAAMGRLTMLASRSVPKLWDEWFAESGTPPVAFSRVQEFEDLLLALGAARSGLGIALAPRA
SIEDDLQRGQLVAPYGFIARPSGYSLCCRTSDAKRPDFSALSGWLRRCGRPS
>Mature_292_residues
MRLPPLNAVRAFEAVCRHGSILKAAEELKVVRGAVRQQIATLESHFGRKLFVRDGRRLIPTAEATAFAEASGAAFDILQR
AACELSGTGPGRIRLGVPSAFAVWWLMPRVADMQASLGDMAVDIVPMSVVEPLLMHPELDAVIMGGEYRPAAGVTAVRFM
EDEFGPVATPALAATLSESPAAMGRLTMLASRSVPKLWDEWFAESGTPPVAFSRVQEFEDLLLALGAARSGLGIALAPRA
SIEDDLQRGQLVAPYGFIARPSGYSLCCRTSDAKRPDFSALSGWLRRCGRPS

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=28.4722222222222, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786448, Length=252, Percent_Identity=28.968253968254, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=25.938566552901, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31378; Mature: 31378

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPPLNAVRAFEAVCRHGSILKAAEELKVVRGAVRQQIATLESHFGRKLFVRDGRRLIP
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEECC
TAEATAFAEASGAAFDILQRAACELSGTGPGRIRLGVPSAFAVWWLMPRVADMQASLGDM
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AVDIVPMSVVEPLLMHPELDAVIMGGEYRPAAGVTAVRFMEDEFGPVATPALAATLSESP
HHHEECHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCC
AAMGRLTMLASRSVPKLWDEWFAESGTPPVAFSRVQEFEDLLLALGAARSGLGIALAPRA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
SIEDDLQRGQLVAPYGFIARPSGYSLCCRTSDAKRPDFSALSGWLRRCGRPS
CCHHHHHCCCEECCCCCEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRLPPLNAVRAFEAVCRHGSILKAAEELKVVRGAVRQQIATLESHFGRKLFVRDGRRLIP
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEECC
TAEATAFAEASGAAFDILQRAACELSGTGPGRIRLGVPSAFAVWWLMPRVADMQASLGDM
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AVDIVPMSVVEPLLMHPELDAVIMGGEYRPAAGVTAVRFMEDEFGPVATPALAATLSESP
HHHEECHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCC
AAMGRLTMLASRSVPKLWDEWFAESGTPPVAFSRVQEFEDLLLALGAARSGLGIALAPRA
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCC
SIEDDLQRGQLVAPYGFIARPSGYSLCCRTSDAKRPDFSALSGWLRRCGRPS
CCHHHHHCCCEECCCCCEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8423001 [H]