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The map label for this gene is fcl [C]

Identifier: 209548033

GI number: 209548033

Start: 436089

End: 437039

Strand: Direct

Name: fcl [C]

Synonym: Rleg2_0426

Alternate gene names: 209548033

Gene position: 436089-437039 (Clockwise)

Preceding gene: 209548032

Following gene: 209548034

Centisome position: 9.61

GC content: 55.84

Gene sequence:

>951_bases
GTGTACGACTTGTCCAACAAGAAGATCTGGGTTGCCGGTCATCGCGGTATGGTCGGCAGTGCTCTTGTGCGCAGGCTTCA
ATCCGAAGATTGCAGCGTCATCACGGCCACGCGCCGTGAGGTCGATTTGAAGCGGCAGGAAGAGGTCGAAAAATTTGTTG
AAGCAAACCGACCCGACGCGATCATTCTCGCCGCTGCCAAGGTTGGCGGTATCCTCGCCAATGACAGTTATCCGGCAGAA
TTCATCTATGACAATCTGATTATCGAAGCGAACATCTTCGAAGCCGCCCACCAGGGGGGAGTGGACCGGCTGCTGTTCCT
GGGTTCAAGCTGCATCTATCCCAAGCTCGCTCCACAGCCGATTCCCGAAGAAGCTCTGTTGACCGGTGCGCTCGAGCCGA
CCAATGAATGGTACGCGATCGCCAAGATCGCCGGTATCAAGCTCGCCGAAGCCTATCGTAAGCAATACGGTCGCGATTAT
ATTTCGGCCATGCCGACCAATCTTTATGGCCCGGGCGATAATTTCGACCTGAATTCCAGCCATGTGCTGCCGGCCCTTAT
CCGCAAGGCGCATGCTGCGAAACTTCGCAAAGACCCTCATATGGTGGTCTGGGGCACCGGGACCCCGCGCCGCGAATTTC
TGCACGTCGATGACTGCGCCGACGCGCTGGTGTTTCTTCTCAAGACATATTCGGGCTCGCAGCACGTCAATGTCGGCTCG
GGAACAGATCTCGAAATTATCGAATTGACGCGGTTGGTCTGCCGCGTTGTCGGTTATGAGGGTGAGATCATTCATGATCT
TTCCAAGCCCGATGGTACGCCGCGAAAGCTGATGAGCAATCAGAAGCTGCAGGACATGGGTTGGAAGCCGCGCATTTCGC
TTGAGGATGGCATCCGAGCCACTTATGCTTGGTTCCTGGAGTTCGAGAATACGTCCGACCCAGCGGTCTGA

Upstream 100 bases:

>100_bases
GGGCTGGCAGCACAAGACCCCGGTTCGCGAGCTCGCCGCCGAAATGGTGCGCGAGGATGTCAAGCACTGGAAGGCGCAAA
ACAGCCGGAAAGAGATCTAA

Downstream 100 bases:

>100_bases
TATCAGGCAAACCAGCATTTATCCCCGCGTCGATCTTCGCGACACAGACTGTGTCGCAGGGCTGGCGCCTGGGCAACATG
CGAGTGATAAGATCGCCGCT

Product: NAD-dependent epimerase/dehydratase

Products: GDP-4-dehydro-6-deoxy-D-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MYDLSNKKIWVAGHRGMVGSALVRRLQSEDCSVITATRREVDLKRQEEVEKFVEANRPDAIILAAAKVGGILANDSYPAE
FIYDNLIIEANIFEAAHQGGVDRLLFLGSSCIYPKLAPQPIPEEALLTGALEPTNEWYAIAKIAGIKLAEAYRKQYGRDY
ISAMPTNLYGPGDNFDLNSSHVLPALIRKAHAAKLRKDPHMVVWGTGTPRREFLHVDDCADALVFLLKTYSGSQHVNVGS
GTDLEIIELTRLVCRVVGYEGEIIHDLSKPDGTPRKLMSNQKLQDMGWKPRISLEDGIRATYAWFLEFENTSDPAV

Sequences:

>Translated_316_residues
MYDLSNKKIWVAGHRGMVGSALVRRLQSEDCSVITATRREVDLKRQEEVEKFVEANRPDAIILAAAKVGGILANDSYPAE
FIYDNLIIEANIFEAAHQGGVDRLLFLGSSCIYPKLAPQPIPEEALLTGALEPTNEWYAIAKIAGIKLAEAYRKQYGRDY
ISAMPTNLYGPGDNFDLNSSHVLPALIRKAHAAKLRKDPHMVVWGTGTPRREFLHVDDCADALVFLLKTYSGSQHVNVGS
GTDLEIIELTRLVCRVVGYEGEIIHDLSKPDGTPRKLMSNQKLQDMGWKPRISLEDGIRATYAWFLEFENTSDPAV
>Mature_316_residues
MYDLSNKKIWVAGHRGMVGSALVRRLQSEDCSVITATRREVDLKRQEEVEKFVEANRPDAIILAAAKVGGILANDSYPAE
FIYDNLIIEANIFEAAHQGGVDRLLFLGSSCIYPKLAPQPIPEEALLTGALEPTNEWYAIAKIAGIKLAEAYRKQYGRDY
ISAMPTNLYGPGDNFDLNSSHVLPALIRKAHAAKLRKDPHMVVWGTGTPRREFLHVDDCADALVFLLKTYSGSQHVNVGS
GTDLEIIELTRLVCRVVGYEGEIIHDLSKPDGTPRKLMSNQKLQDMGWKPRISLEDGIRATYAWFLEFENTSDPAV

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=312, Percent_Identity=31.0897435897436, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI7657641, Length=334, Percent_Identity=20.9580838323353, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI42516563, Length=323, Percent_Identity=26.0061919504644, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788365, Length=317, Percent_Identity=55.5205047318612, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=23.5632183908046, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=22.4137931034483, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554428, Length=311, Percent_Identity=30.8681672025724, Blast_Score=163, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI115532424, Length=276, Percent_Identity=25.3623188405797, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922778, Length=315, Percent_Identity=30.1587301587302, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21356223, Length=320, Percent_Identity=25.3125, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271

Molecular weight: Translated: 35170; Mature: 35170

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDLSNKKIWVAGHRGMVGSALVRRLQSEDCSVITATRREVDLKRQEEVEKFVEANRPDA
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHCCCCCE
IILAAAKVGGILANDSYPAEFIYDNLIIEANIFEAAHQGGVDRLLFLGSSCIYPKLAPQP
EEEEEHHHCCEEECCCCCHHHHHCCEEEEEHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC
IPEEALLTGALEPTNEWYAIAKIAGIKLAEAYRKQYGRDYISAMPTNLYGPGDNFDLNSS
CCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCC
HVLPALIRKAHAAKLRKDPHMVVWGTGTPRREFLHVDDCADALVFLLKTYSGSQHVNVGS
CHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHEEEHHHHHHHHHHHHHHCCCCCEEECCC
GTDLEIIELTRLVCRVVGYEGEIIHDLSKPDGTPRKLMSNQKLQDMGWKPRISLEDGIRA
CCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHCCCCHHHCCCCCCCCHHCCCEE
TYAWFLEFENTSDPAV
EEEEEEEECCCCCCCC
>Mature Secondary Structure
MYDLSNKKIWVAGHRGMVGSALVRRLQSEDCSVITATRREVDLKRQEEVEKFVEANRPDA
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHHHCCHHHHHHHHHHHCCCCCE
IILAAAKVGGILANDSYPAEFIYDNLIIEANIFEAAHQGGVDRLLFLGSSCIYPKLAPQP
EEEEEHHHCCEEECCCCCHHHHHCCEEEEEHHHHHHHHCCHHHHHHHCCCCCCCCCCCCC
IPEEALLTGALEPTNEWYAIAKIAGIKLAEAYRKQYGRDYISAMPTNLYGPGDNFDLNSS
CCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCC
HVLPALIRKAHAAKLRKDPHMVVWGTGTPRREFLHVDDCADALVFLLKTYSGSQHVNVGS
CHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHEEEHHHHHHHHHHHHHHCCCCCEEECCC
GTDLEIIELTRLVCRVVGYEGEIIHDLSKPDGTPRKLMSNQKLQDMGWKPRISLEDGIRA
CCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHCCCCHHHCCCCCCCCHHCCCEE
TYAWFLEFENTSDPAV
EEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-L-fucose; NADP+

Specific reaction: GDP-L-fucose + NADP+ = GDP-4-dehydro-6-deoxy-D-mannose + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]