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The map label for this gene is noeL [H]

Identifier: 209548032

GI number: 209548032

Start: 435027

End: 436088

Strand: Direct

Name: noeL [H]

Synonym: Rleg2_0425

Alternate gene names: 209548032

Gene position: 435027-436088 (Clockwise)

Preceding gene: 209548031

Following gene: 209548033

Centisome position: 9.59

GC content: 57.72

Gene sequence:

>1062_bases
ATGACGAAAACCGCGCTGATTACTGGGGTAACTGGTCAGGATGGGGCCTATCTGGCCGAATTGCTTCTGAGCAAGGGCTA
TACGGTGCATGGTATCAAGCGCCGGTCGTCGTCTTTCAATACCGGCCGCATCGAGCATATTTACCAGGATCCGCACGAGG
CGCATCCACGCTTCATTCTGCACTATGGCGATATGATCGACTCGACCAATCTCCTGCGGATCGTGCAGCAGACGCAGCCC
GATGAAATCTACAATCTTGCCGCCCAGAGCCATGTCGGTGTCAGCTTCGAAACGCCTGAATATACGGCGGATGCCGATGG
TATCGGGACGTTGAGGCTGCTCGAGGCGATCCGTATCCTGCGGCTCGAGGAAAAGACCCGGTTCTACCAGGCATCGACGT
CGGAACTCTATGGTCTTGTGCAGGAAGTGCCGCAGAGCGAGAAGACGCCGTTTTACCCGCGTTCTCCCTATGCCGCAGCC
AAGCTCTATGCCTACTGGATCGTGGTGAATTATCGCGAAGCCTACGGCATGCATGCGTCGAACGGCATTCTTTTCAATCA
CGAAAGCCCGCTTCGCGGCGAGACCTTCGTCACGCGCAAGATCACGATGGCCGTAGCCGCCATCCATCTCGGCCTTCAGG
ATAAGCTCTTCCTCGGCAATCTTGACGCCAAGCGAGACTGGGGTCATGCACGTGAGTATGTTGAAGGCATGTGGCGCATG
CTGCAGCAGGACAAGCCGGATGACTATGTCTTGGCGACAGGTGAGACAACGAGTGTCCGCCAGTTTGTCGAGTGGGCGTT
TGCCGACGTCGGCATTGCTCTGGAATGGAAGGGGTCCGGCGTCGACGAAAAGGGTTACGATTCCGCGTCGGGTGCGTGCC
TCGTGGAAGTCGATCCTCGGTATTTCCGCCCGACGGAAGTCGATCTTCTGCTGGGTGATCCGACCAAGGCACGCCAGAAG
CTGGGCTGGCAGCACAAGACCCCGGTTCGCGAGCTCGCCGCCGAAATGGTGCGCGAGGATGTCAAGCACTGGAAGGCGCA
AAACAGCCGGAAAGAGATCTAA

Upstream 100 bases:

>100_bases
GATGCATTTCGCTGGCCTGGCCGGGTCACATGGGGGTTGCGCTAGTTCGGCCGTTTCCATAAACACGTCCCGAAGATTGT
ACCGAAAGACGATGTGAAAA

Downstream 100 bases:

>100_bases
GTGTACGACTTGTCCAACAAGAAGATCTGGGTTGCCGGTCATCGCGGTATGGTCGGCAGTGCTCTTGTGCGCAGGCTTCA
ATCCGAAGATTGCAGCGTCA

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTKTALITGVTGQDGAYLAELLLSKGYTVHGIKRRSSSFNTGRIEHIYQDPHEAHPRFILHYGDMIDSTNLLRIVQQTQP
DEIYNLAAQSHVGVSFETPEYTADADGIGTLRLLEAIRILRLEEKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAAA
KLYAYWIVVNYREAYGMHASNGILFNHESPLRGETFVTRKITMAVAAIHLGLQDKLFLGNLDAKRDWGHAREYVEGMWRM
LQQDKPDDYVLATGETTSVRQFVEWAFADVGIALEWKGSGVDEKGYDSASGACLVEVDPRYFRPTEVDLLLGDPTKARQK
LGWQHKTPVRELAAEMVREDVKHWKAQNSRKEI

Sequences:

>Translated_353_residues
MTKTALITGVTGQDGAYLAELLLSKGYTVHGIKRRSSSFNTGRIEHIYQDPHEAHPRFILHYGDMIDSTNLLRIVQQTQP
DEIYNLAAQSHVGVSFETPEYTADADGIGTLRLLEAIRILRLEEKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAAA
KLYAYWIVVNYREAYGMHASNGILFNHESPLRGETFVTRKITMAVAAIHLGLQDKLFLGNLDAKRDWGHAREYVEGMWRM
LQQDKPDDYVLATGETTSVRQFVEWAFADVGIALEWKGSGVDEKGYDSASGACLVEVDPRYFRPTEVDLLLGDPTKARQK
LGWQHKTPVRELAAEMVREDVKHWKAQNSRKEI
>Mature_352_residues
TKTALITGVTGQDGAYLAELLLSKGYTVHGIKRRSSSFNTGRIEHIYQDPHEAHPRFILHYGDMIDSTNLLRIVQQTQPD
EIYNLAAQSHVGVSFETPEYTADADGIGTLRLLEAIRILRLEEKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAAAK
LYAYWIVVNYREAYGMHASNGILFNHESPLRGETFVTRKITMAVAAIHLGLQDKLFLGNLDAKRDWGHAREYVEGMWRML
QQDKPDDYVLATGETTSVRQFVEWAFADVGIALEWKGSGVDEKGYDSASGACLVEVDPRYFRPTEVDLLLGDPTKARQKL
GWQHKTPVRELAAEMVREDVKHWKAQNSRKEI

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=62.280701754386, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI1788366, Length=361, Percent_Identity=65.6509695290859, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1788353, Length=205, Percent_Identity=27.8048780487805, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI48994969, Length=198, Percent_Identity=29.2929292929293, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786974, Length=360, Percent_Identity=25, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=65.6891495601173, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=65.6891495601173, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=65.6891495601173, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=65.6891495601173, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=65.6891495601173, Blast_Score=474, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=66.5689149560117, Blast_Score=473, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24158427, Length=342, Percent_Identity=61.6959064327485, Blast_Score=449, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 40073; Mature: 39942

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTALITGVTGQDGAYLAELLLSKGYTVHGIKRRSSSFNTGRIEHIYQDPHEAHPRFIL
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEHHCCCCCCCCCHHHHHCCCCCCCCCEEE
HYGDMIDSTNLLRIVQQTQPDEIYNLAAQSHVGVSFETPEYTADADGIGTLRLLEAIRIL
EECCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
RLEEKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAAAKLYAYWIVVNYREAYGMHAS
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHCCCCCC
NGILFNHESPLRGETFVTRKITMAVAAIHLGLQDKLFLGNLDAKRDWGHAREYVEGMWRM
CCEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
LQQDKPDDYVLATGETTSVRQFVEWAFADVGIALEWKGSGVDEKGYDSASGACLVEVDPR
HHCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCC
YFRPTEVDLLLGDPTKARQKLGWQHKTPVRELAAEMVREDVKHWKAQNSRKEI
CCCCCEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TKTALITGVTGQDGAYLAELLLSKGYTVHGIKRRSSSFNTGRIEHIYQDPHEAHPRFIL
CCCEEEEECCCCCHHHHHHHHHHCCCEEEEEHHCCCCCCCCCHHHHHCCCCCCCCCEEE
HYGDMIDSTNLLRIVQQTQPDEIYNLAAQSHVGVSFETPEYTADADGIGTLRLLEAIRIL
EECCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHH
RLEEKTRFYQASTSELYGLVQEVPQSEKTPFYPRSPYAAAKLYAYWIVVNYREAYGMHAS
HHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEEEEEEEEEEHHHCCCCCC
NGILFNHESPLRGETFVTRKITMAVAAIHLGLQDKLFLGNLDAKRDWGHAREYVEGMWRM
CCEEECCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHH
LQQDKPDDYVLATGETTSVRQFVEWAFADVGIALEWKGSGVDEKGYDSASGACLVEVDPR
HHCCCCCCEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEEEEECCC
YFRPTEVDLLLGDPTKARQKLGWQHKTPVRELAAEMVREDVKHWKAQNSRKEI
CCCCCEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10065558 [H]