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The map label for this gene is rbsB [H]

Identifier: 209547762

GI number: 209547762

Start: 147731

End: 148720

Strand: Reverse

Name: rbsB [H]

Synonym: Rleg2_0151

Alternate gene names: 209547762

Gene position: 148720-147731 (Counterclockwise)

Preceding gene: 209547763

Following gene: 209547761

Centisome position: 3.28

GC content: 62.32

Gene sequence:

>990_bases
ATGACCATCGTGAAATCCCTTCTGTCGCGCCGCGCCTTTACCGCGCTCGCCGGCGCCGCCGTCATCGCCACGGCGATGCC
GGTCACGTCCTTTGCCGCCGACGTGACGATCCCGATCATCGTCAAGGACACGACGTCCTTCTACTGGCAGATCGTTCTGG
CCGGCGCCCGCAAGGCCGGCAAGGATCTCGGCGTCAACGTGCCGGAACTCGGCGCCCAGGCGGAATCCGACATCAACGGC
CAGATCAGCATTCTGGAGAACGCCGTTGCCGGCAAACCGGCCGCCGTCGTCATTTCGCCGACCGAATTCAAGGCGCTCGG
CAAGCCGATCGATGAAGCCGCCAAGTCGGTTCCGATCATCGGCATCGATTCAGGCGCCGACTCCAAGGCGTTCAAGTCGT
TCCTGACGACCGACAACGTCCAGGGCGGTCGCATCGCCGCCGACGGTCTTGCCGCCGCCATCAAGGAGATGACGGGCAAG
GAAGAAGGCGAAATCGTCATCCTCACCAATCTTCCCGGCGTCGGCTCGCTGGAGCAGCGCCGCGAAGGCTTCCTGGATCA
GATCAAGACCAAGCATCCGGGCCTCAAGGTCATCGCCGACAAGTATGGCGACGGCCAGGCAACGACCGGCCTCAACATGA
TGACCGACCTGATTACGGCCAATCCGAAGCTCGTCGGCGTCTTCGCCTCGAACCTGATCCTGGCGCAGGGTGTTGGTCAG
GCGATCGCCGAAAACAAGCTCGGCGACAAGATCAAGGTCATCGGCTTCGACAGCGACGACAAGACGGTCGGCTTCCTCAA
GGACGGCGCCATTGCCGGTCTCGTCGTCCAGGACCCCTACCGCATGGGTTATGACGGCATCAAGACCGCGCTTGCCGTCT
CCAAGGGCGAGAAGGTCGAAGCCAATGTCGATACCGGCGCCAACCTCGTCACCAAGGCGAATATGGCCGATCCGAAGATC
GACGCCCTGCTGAACCCGAAGATCAAGTAA

Upstream 100 bases:

>100_bases
TAGCTTCTCCCTGCAAATGTTTTTTATCGATAAAAATCGTCCGCCTGCGTTCAGCGGCCGGTTTATTCGGAGGAGAACGC
ATCCTGAGAGGAGAACCCTT

Downstream 100 bases:

>100_bases
ACAAGACAGACAGGCCGCGCCGGTTTTTAAAAACCGGCGCGGCTTTTTCCATCATGCTATGGTTTGCAAGGGTGGAAATG
AGGCGACCAAGAGGAGGAGA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTIVKSLLSRRAFTALAGAAVIATAMPVTSFAADVTIPIIVKDTTSFYWQIVLAGARKAGKDLGVNVPELGAQAESDING
QISILENAVAGKPAAVVISPTEFKALGKPIDEAAKSVPIIGIDSGADSKAFKSFLTTDNVQGGRIAADGLAAAIKEMTGK
EEGEIVILTNLPGVGSLEQRREGFLDQIKTKHPGLKVIADKYGDGQATTGLNMMTDLITANPKLVGVFASNLILAQGVGQ
AIAENKLGDKIKVIGFDSDDKTVGFLKDGAIAGLVVQDPYRMGYDGIKTALAVSKGEKVEANVDTGANLVTKANMADPKI
DALLNPKIK

Sequences:

>Translated_329_residues
MTIVKSLLSRRAFTALAGAAVIATAMPVTSFAADVTIPIIVKDTTSFYWQIVLAGARKAGKDLGVNVPELGAQAESDING
QISILENAVAGKPAAVVISPTEFKALGKPIDEAAKSVPIIGIDSGADSKAFKSFLTTDNVQGGRIAADGLAAAIKEMTGK
EEGEIVILTNLPGVGSLEQRREGFLDQIKTKHPGLKVIADKYGDGQATTGLNMMTDLITANPKLVGVFASNLILAQGVGQ
AIAENKLGDKIKVIGFDSDDKTVGFLKDGAIAGLVVQDPYRMGYDGIKTALAVSKGEKVEANVDTGANLVTKANMADPKI
DALLNPKIK
>Mature_328_residues
TIVKSLLSRRAFTALAGAAVIATAMPVTSFAADVTIPIIVKDTTSFYWQIVLAGARKAGKDLGVNVPELGAQAESDINGQ
ISILENAVAGKPAAVVISPTEFKALGKPIDEAAKSVPIIGIDSGADSKAFKSFLTTDNVQGGRIAADGLAAAIKEMTGKE
EGEIVILTNLPGVGSLEQRREGFLDQIKTKHPGLKVIADKYGDGQATTGLNMMTDLITANPKLVGVFASNLILAQGVGQA
IAENKLGDKIKVIGFDSDDKTVGFLKDGAIAGLVVQDPYRMGYDGIKTALAVSKGEKVEANVDTGANLVTKANMADPKID
ALLNPKIK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=309, Percent_Identity=28.4789644012945, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790526, Length=282, Percent_Identity=26.5957446808511, Blast_Score=88, Evalue=7e-19,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34187; Mature: 34056

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVKSLLSRRAFTALAGAAVIATAMPVTSFAADVTIPIIVKDTTSFYWQIVLAGARKAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHEEHHHHHHCHHCC
KDLGVNVPELGAQAESDINGQISILENAVAGKPAAVVISPTEFKALGKPIDEAAKSVPII
CCCCCCCHHCCCCCCCCCCCEEEEEHHHHCCCCEEEEECCHHHHHHCCCHHHHHHCCCEE
GIDSGADSKAFKSFLTTDNVQGGRIAADGLAAAIKEMTGKEEGEIVILTNLPGVGSLEQR
EECCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
REGFLDQIKTKHPGLKVIADKYGDGQATTGLNMMTDLITANPKLVGVFASNLILAQGVGQ
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
AIAENKLGDKIKVIGFDSDDKTVGFLKDGAIAGLVVQDPYRMGYDGIKTALAVSKGEKVE
HHHHHCCCCEEEEEEECCCCCEEEEEECCCEEEEEEECCHHCCHHHHHHHHHHCCCCEEE
ANVDTGANLVTKANMADPKIDALLNPKIK
ECCCCCCCEEEECCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
TIVKSLLSRRAFTALAGAAVIATAMPVTSFAADVTIPIIVKDTTSFYWQIVLAGARKAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCHHEEHHHHHHCHHCC
KDLGVNVPELGAQAESDINGQISILENAVAGKPAAVVISPTEFKALGKPIDEAAKSVPII
CCCCCCCHHCCCCCCCCCCCEEEEEHHHHCCCCEEEEECCHHHHHHCCCHHHHHHCCCEE
GIDSGADSKAFKSFLTTDNVQGGRIAADGLAAAIKEMTGKEEGEIVILTNLPGVGSLEQR
EECCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
REGFLDQIKTKHPGLKVIADKYGDGQATTGLNMMTDLITANPKLVGVFASNLILAQGVGQ
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
AIAENKLGDKIKVIGFDSDDKTVGFLKDGAIAGLVVQDPYRMGYDGIKTALAVSKGEKVE
HHHHHCCCCEEEEEEECCCCCEEEEEECCCEEEEEEECCHHCCHHHHHHHHHHCCCCEEE
ANVDTGANLVTKANMADPKIDALLNPKIK
ECCCCCCCEEEECCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]