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The map label for this gene is ada [H]

Identifier: 209547678

GI number: 209547678

Start: 66160

End: 67233

Strand: Direct

Name: ada [H]

Synonym: Rleg2_0066

Alternate gene names: 209547678

Gene position: 66160-67233 (Clockwise)

Preceding gene: 209547675

Following gene: 209547679

Centisome position: 1.46

GC content: 63.69

Gene sequence:

>1074_bases
ATGCTTTTCGAACGCCCCGACGATGATACGCTCTATGACGCCCTGATCGCGCGCAGCGCCGATTACGAGGGCCAGGCCTA
TGTCTGCGTCAAGACGACGGGCATCTTCTGCCGGCTGACCTGTCCGGCGCGGAAACCGAAGCGGGAAAATACTCTGTTCT
TCGAGACTATTGCCGCCTGCATGCATTCCGGCTTTCGCCCCTGCCGGCGCTGCAGGCCGCTGGAACAGCCGGGCAGGGAG
CCGATCGTCGACGAGCTGCTTGCGGCACTCGACGCTCAGCCGGAGGTTCGCTGGACCGAGGAGGAGATTGTCCGCCGCGG
CCATGATCCCTTGACGGTGCGCCGCGCCTTCAAGCGGGCGCTCGGCATGACTTTCCATGACATCGTCCGCTATATCAGGC
TCGGCGAAGCGGCACGGCAACTCGCCGACGGGGCGCGTGTCATCGATGCGCAGATCGACGCCGGATACGAGTCTCCGAGC
GGCTTTCGCACGGCATTCCAGCGGCTCGTCGGCCAGGCGCCGGCGCTTTCGCAAAACCGCGAACTGCTCTTTGCCGATTG
GTTCGACACCCCGCTCGGGCCGATGATCGCGGTTGCCGACAAGACGCATCTGCATCTTCTCGAATTTCATGATCGTAAGG
CGCTGCCGACCGAGCTCGACGCGCTGCAGAAGCGCGTCCGCTCCTCGGTCGCAATCGGCCGCACACCCGTCATCGATCAG
ATCGAGGCGGAGATCCGGAATTATTTCGAGGGCCGGCTCACCGTTTTCACGACGCCCCTGGCGCTTGGCGGCACGCCCTT
CGAGAAACATGTCTGGGCAAAGCTGATGGAAATCCCTGTCGGCAAGACGCGCGCCTATGGCGATCTGGCACGGGAGATGG
AAAGGCCGGAGGTGGTGCGGGCCGTCGGCCGCGCCAACGGCGCCAATCAGCTGGCCATCATCGTGCCCTGCCATCGCATT
CTCGGCGCGGATGGTTCTCTCACAGGTTACGGCGGCGGGCTCTGGCGCAAACAGTGGCTGCTGCGGCATGAAGAGAAGAT
CGGGACGCAAGCAAAGATGGAGGAGACAACATGA

Upstream 100 bases:

>100_bases
GGTCTTATGGCATGGGTGCGGGCGCAAGGAAATCGACAAGAATAAAATAGACGCCCGATTTTCGGCGGACGGCCCATGCG
CGGGATGCTAGAAAGCCGAT

Downstream 100 bases:

>100_bases
ACCAGACTGAAAAGGCCAAGGCATTCCACGCGCTGCATAAGAAGGGCGATCCGATTGTCCTCTACAATATCTGGGATGCC
GGCACGGCGAAGGCGGTTGC

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLFERPDDDTLYDALIARSADYEGQAYVCVKTTGIFCRLTCPARKPKRENTLFFETIAACMHSGFRPCRRCRPLEQPGRE
PIVDELLAALDAQPEVRWTEEEIVRRGHDPLTVRRAFKRALGMTFHDIVRYIRLGEAARQLADGARVIDAQIDAGYESPS
GFRTAFQRLVGQAPALSQNRELLFADWFDTPLGPMIAVADKTHLHLLEFHDRKALPTELDALQKRVRSSVAIGRTPVIDQ
IEAEIRNYFEGRLTVFTTPLALGGTPFEKHVWAKLMEIPVGKTRAYGDLAREMERPEVVRAVGRANGANQLAIIVPCHRI
LGADGSLTGYGGGLWRKQWLLRHEEKIGTQAKMEETT

Sequences:

>Translated_357_residues
MLFERPDDDTLYDALIARSADYEGQAYVCVKTTGIFCRLTCPARKPKRENTLFFETIAACMHSGFRPCRRCRPLEQPGRE
PIVDELLAALDAQPEVRWTEEEIVRRGHDPLTVRRAFKRALGMTFHDIVRYIRLGEAARQLADGARVIDAQIDAGYESPS
GFRTAFQRLVGQAPALSQNRELLFADWFDTPLGPMIAVADKTHLHLLEFHDRKALPTELDALQKRVRSSVAIGRTPVIDQ
IEAEIRNYFEGRLTVFTTPLALGGTPFEKHVWAKLMEIPVGKTRAYGDLAREMERPEVVRAVGRANGANQLAIIVPCHRI
LGADGSLTGYGGGLWRKQWLLRHEEKIGTQAKMEETT
>Mature_357_residues
MLFERPDDDTLYDALIARSADYEGQAYVCVKTTGIFCRLTCPARKPKRENTLFFETIAACMHSGFRPCRRCRPLEQPGRE
PIVDELLAALDAQPEVRWTEEEIVRRGHDPLTVRRAFKRALGMTFHDIVRYIRLGEAARQLADGARVIDAQIDAGYESPS
GFRTAFQRLVGQAPALSQNRELLFADWFDTPLGPMIAVADKTHLHLLEFHDRKALPTELDALQKRVRSSVAIGRTPVIDQ
IEAEIRNYFEGRLTVFTTPLALGGTPFEKHVWAKLMEIPVGKTRAYGDLAREMERPEVVRAVGRANGANQLAIIVPCHRI
LGADGSLTGYGGGLWRKQWLLRHEEKIGTQAKMEETT

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=164, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1788542, Length=346, Percent_Identity=33.5260115606936, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1787596, Length=164, Percent_Identity=40.8536585365854, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI115533068, Length=91, Percent_Identity=43.956043956044, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=91, Percent_Identity=43.956043956044, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=43.5294117647059, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 40235; Mature: 40235

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFERPDDDTLYDALIARSADYEGQAYVCVKTTGIFCRLTCPARKPKRENTLFFETIAAC
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHH
MHSGFRPCRRCRPLEQPGREPIVDELLAALDAQPEVRWTEEEIVRRGHDPLTVRRAFKRA
HHCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEECHHHHHHCCCCCHHHHHHHHHH
LGMTFHDIVRYIRLGEAARQLADGARVIDAQIDAGYESPSGFRTAFQRLVGQAPALSQNR
HCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCC
ELLFADWFDTPLGPMIAVADKTHLHLLEFHDRKALPTELDALQKRVRSSVAIGRTPVIDQ
CEEEEECCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
IEAEIRNYFEGRLTVFTTPLALGGTPFEKHVWAKLMEIPVGKTRAYGDLAREMERPEVVR
HHHHHHHHHCCCEEEEECCHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
AVGRANGANQLAIIVPCHRILGADGSLTGYGGGLWRKQWLLRHEEKIGTQAKMEETT
HHHCCCCCCCEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLFERPDDDTLYDALIARSADYEGQAYVCVKTTGIFCRLTCPARKPKRENTLFFETIAAC
CCCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHH
MHSGFRPCRRCRPLEQPGREPIVDELLAALDAQPEVRWTEEEIVRRGHDPLTVRRAFKRA
HHCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEECHHHHHHCCCCCHHHHHHHHHH
LGMTFHDIVRYIRLGEAARQLADGARVIDAQIDAGYESPSGFRTAFQRLVGQAPALSQNR
HCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCC
ELLFADWFDTPLGPMIAVADKTHLHLLEFHDRKALPTELDALQKRVRSSVAIGRTPVIDQ
CEEEEECCCCCCCCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHH
IEAEIRNYFEGRLTVFTTPLALGGTPFEKHVWAKLMEIPVGKTRAYGDLAREMERPEVVR
HHHHHHHHHCCCEEEEECCHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
AVGRANGANQLAIIVPCHRILGADGSLTGYGGGLWRKQWLLRHEEKIGTQAKMEETT
HHHCCCCCCCEEEEEEHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]