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The map label for this gene is prpB [C]

Identifier: 209547679

GI number: 209547679

Start: 67230

End: 68000

Strand: Direct

Name: prpB [C]

Synonym: Rleg2_0067

Alternate gene names: 209547679

Gene position: 67230-68000 (Clockwise)

Preceding gene: 209547678

Following gene: 209547682

Centisome position: 1.48

GC content: 62.91

Gene sequence:

>771_bases
ATGAACCAGACTGAAAAGGCCAAGGCATTCCACGCGCTGCATAAGAAGGGCGATCCGATTGTCCTCTACAATATCTGGGA
TGCCGGCACGGCGAAGGCGGTTGCCGATGCCGGCGCCAAGGCGCTCGCGACCGGAAGCTGGTCGGTCGCAGCAGCCCACG
GGTTTGCCGATGGCGAGAAACTGCCGATGCCGGTGCTTGTGGAGATGGCTAAATCCATCACCGACGTCATCGACCTGCCG
CTGTCGGTCGATTTCGAAGGCGCCTATTCGACCGAGCCCGAGGGGGCGGCCGCCAATGTCGCCAGGCTCGTGGAAATCGG
CGCCATCGGCATCAACTTCGAAGATCGGGTGGATGCCGGCGAGGGGCTCTATCCCGTCGAGAGCCAGGCAGCCCGCATCC
GCGCCATTCGCGCCATGGCCGATGGCAAGGATATCCCCTTCTTCATCAACGCCCGCACCGATCTCTTCCTGGCCGAGGGC
GATCTTGCCAAACATGCCGGTCTGGTCGACGAGGCAATCGAGCGAGGCAGGGCCTATGCGGCCGCCGGCGGCAACGGTTT
CTTCGTTCCCGGCCTGATCGATCCCGCCTTGATCGAAAAGATCTGCGCGGCATCGTCGCTGCCGGTCAATATCATGATGC
GGGCGGGCGCCCCTGATGTGAAAACCCTGGCGAAGCTCGGCGTCGGCCGCATCAGCTATGGCCCGGGACCTTACCGGTCG
ATGATGGAAAAGCTGAAGCAGGAGGCGGCGGCGATTTATAGCCTGCTCTGA

Upstream 100 bases:

>100_bases
ATGGTTCTCTCACAGGTTACGGCGGCGGGCTCTGGCGCAAACAGTGGCTGCTGCGGCATGAAGAGAAGATCGGGACGCAA
GCAAAGATGGAGGAGACAAC

Downstream 100 bases:

>100_bases
CGCCGCTATGTCAGAGGGCCGCAGCCGGATGCAAGGCTGCGGCCCTTAAATGACATCCTGTTCCGCTCAGGAACGGAAGC
GCAGATTGCGCCGTGTCAGC

Product: PEP phosphonomutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNQTEKAKAFHALHKKGDPIVLYNIWDAGTAKAVADAGAKALATGSWSVAAAHGFADGEKLPMPVLVEMAKSITDVIDLP
LSVDFEGAYSTEPEGAAANVARLVEIGAIGINFEDRVDAGEGLYPVESQAARIRAIRAMADGKDIPFFINARTDLFLAEG
DLAKHAGLVDEAIERGRAYAAAGGNGFFVPGLIDPALIEKICAASSLPVNIMMRAGAPDVKTLAKLGVGRISYGPGPYRS
MMEKLKQEAAAIYSLL

Sequences:

>Translated_256_residues
MNQTEKAKAFHALHKKGDPIVLYNIWDAGTAKAVADAGAKALATGSWSVAAAHGFADGEKLPMPVLVEMAKSITDVIDLP
LSVDFEGAYSTEPEGAAANVARLVEIGAIGINFEDRVDAGEGLYPVESQAARIRAIRAMADGKDIPFFINARTDLFLAEG
DLAKHAGLVDEAIERGRAYAAAGGNGFFVPGLIDPALIEKICAASSLPVNIMMRAGAPDVKTLAKLGVGRISYGPGPYRS
MMEKLKQEAAAIYSLL
>Mature_256_residues
MNQTEKAKAFHALHKKGDPIVLYNIWDAGTAKAVADAGAKALATGSWSVAAAHGFADGEKLPMPVLVEMAKSITDVIDLP
LSVDFEGAYSTEPEGAAANVARLVEIGAIGINFEDRVDAGEGLYPVESQAARIRAIRAMADGKDIPFFINARTDLFLAEG
DLAKHAGLVDEAIERGRAYAAAGGNGFFVPGLIDPALIEKICAASSLPVNIMMRAGAPDVKTLAKLGVGRISYGPGPYRS
MMEKLKQEAAAIYSLL

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=258, Percent_Identity=30.2325581395349, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 26913; Mature: 26913

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTEKAKAFHALHKKGDPIVLYNIWDAGTAKAVADAGAKALATGSWSVAAAHGFADGEK
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCEEECCCCEEEEECCCCCCCC
LPMPVLVEMAKSITDVIDLPLSVDFEGAYSTEPEGAAANVARLVEIGAIGINFEDRVDAG
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCEEECCCHHCCCCC
EGLYPVESQAARIRAIRAMADGKDIPFFINARTDLFLAEGDLAKHAGLVDEAIERGRAYA
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHCCHHHHHHHCCCEEE
AAGGNGFFVPGLIDPALIEKICAASSLPVNIMMRAGAPDVKTLAKLGVGRISYGPGPYRS
ECCCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCHHHH
MMEKLKQEAAAIYSLL
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQTEKAKAFHALHKKGDPIVLYNIWDAGTAKAVADAGAKALATGSWSVAAAHGFADGEK
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCEEECCCCEEEEECCCCCCCC
LPMPVLVEMAKSITDVIDLPLSVDFEGAYSTEPEGAAANVARLVEIGAIGINFEDRVDAG
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCEEECCCHHCCCCC
EGLYPVESQAARIRAIRAMADGKDIPFFINARTDLFLAEGDLAKHAGLVDEAIERGRAYA
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCHHHHCCHHHHHHHCCCEEE
AAGGNGFFVPGLIDPALIEKICAASSLPVNIMMRAGAPDVKTLAKLGVGRISYGPGPYRS
ECCCCCEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCHHHH
MMEKLKQEAAAIYSLL
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]