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The map label for this gene is ptsN [H]

Identifier: 209547663

GI number: 209547663

Start: 51898

End: 52362

Strand: Direct

Name: ptsN [H]

Synonym: Rleg2_0051

Alternate gene names: 209547663

Gene position: 51898-52362 (Clockwise)

Preceding gene: 209547662

Following gene: 209547667

Centisome position: 1.14

GC content: 61.51

Gene sequence:

>465_bases
ATGGCATTGGCAGATTTGCTCCATCAGGATGCGATCATTCCCGCCCTCAGAGTAAATTCCAAGAAACAGTTGCTTCAGGA
ATTGGCCGCAAGAGCCGCCAGGATCACCGGGCTCTCCGAACGGGAGATCTTCGACGTCATCCTGCAGCGCGAACGCCTTG
GCTCGACCGGTGTCGGCAACGGTATCGCCATTCCTCACGGCAAGCTGGTCAACATCCATTCGATCGTCGGCATCTTCGCC
CGGCTGGAGCAGCCGGTCGATTTCGAAGCCCTGGACGACCAGCCTGTCGACCTCGTCTTCCTGCTGCTGGCGCCGGAAGG
CGCGGGCGCCGATCATCTCAAGGCCCTGTCGCGGATCGCCCGCGTGCTGCGCGATCACGATCTGGTCGCCAAGCTGCGCG
CCACGGATTCCGCTTCGGCAATCTACGCCTTCCTCAACGAAGAGCAGACGTCGAACGCTGCCTGA

Upstream 100 bases:

>100_bases
CTGGATCGATTCAGCCAATATCAAAGGCTGAGATCAGGCGGTGAGCGGCGGTATCGCCGCCGCTCGTTGCATTTGATCCC
GGCGACAGAAGGAAAAAGAA

Downstream 100 bases:

>100_bases
GCGTCATTTATGCCTGAATGCAAAAGGCGCCGGCTCAGCCCGGCGCCTTTTGCATGATAAGGACCTGATGCTTAGAACTC
TTCCCAGCTATCCTGCGCCA

Product: PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MALADLLHQDAIIPALRVNSKKQLLQELAARAARITGLSEREIFDVILQRERLGSTGVGNGIAIPHGKLVNIHSIVGIFA
RLEQPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIARVLRDHDLVAKLRATDSASAIYAFLNEEQTSNAA

Sequences:

>Translated_154_residues
MALADLLHQDAIIPALRVNSKKQLLQELAARAARITGLSEREIFDVILQRERLGSTGVGNGIAIPHGKLVNIHSIVGIFA
RLEQPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIARVLRDHDLVAKLRATDSASAIYAFLNEEQTSNAA
>Mature_153_residues
ALADLLHQDAIIPALRVNSKKQLLQELAARAARITGLSEREIFDVILQRERLGSTGVGNGIAIPHGKLVNIHSIVGIFAR
LEQPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIARVLRDHDLVAKLRATDSASAIYAFLNEEQTSNAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=150, Percent_Identity=39.3333333333333, Blast_Score=115, Evalue=1e-27,
Organism=Escherichia coli, GI1786951, Length=140, Percent_Identity=32.1428571428571, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI2367327, Length=134, Percent_Identity=28.3582089552239, Blast_Score=66, Evalue=9e-13,
Organism=Escherichia coli, GI1788726, Length=135, Percent_Identity=34.0740740740741, Blast_Score=63, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16675; Mature: 16544

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALADLLHQDAIIPALRVNSKKQLLQELAARAARITGLSEREIFDVILQRERLGSTGVGN
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLVNIHSIVGIFARLEQPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIA
CEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
RVLRDHDLVAKLRATDSASAIYAFLNEEQTSNAA
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALADLLHQDAIIPALRVNSKKQLLQELAARAARITGLSEREIFDVILQRERLGSTGVGN
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
GIAIPHGKLVNIHSIVGIFARLEQPVDFEALDDQPVDLVFLLLAPEGAGADHLKALSRIA
CEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
RVLRDHDLVAKLRATDSASAIYAFLNEEQTSNAA
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]