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The map label for this gene is def [H]

Identifier: 209547667

GI number: 209547667

Start: 57537

End: 58052

Strand: Direct

Name: def [H]

Synonym: Rleg2_0055

Alternate gene names: 209547667

Gene position: 57537-58052 (Clockwise)

Preceding gene: 209547663

Following gene: 209547668

Centisome position: 1.27

GC content: 59.5

Gene sequence:

>516_bases
ATGACCATCAAGCCACTCATCATTCTTCCCGATCCTATTCTCCGCCAGGCCTCCAAGCCGATCGAGCGGGTCGATGCCGA
TCTGCAGGGTCTTGCCGACGATATGCTGGAAACCATGTACGACGCGCCGGGCATCGGCCTTGCGGCGATCCAGATCGGCG
TGCCGCGCCGCATGCTGGTCATCGACGTGTCGCGCGAGGGCGAGGAAAAGCAGCCGCAGGTCTTCATCAACCCGGAGATC
GTCAAATCCTCCGACGAGCGCTCGGTCTATGAGGAAGGCTGCCTGTCTATCCCGGATTATTATGCCGAGGTCGAACGCCC
CGCTGTCGTCTCGGTCAAATTTCTCGACCGGAACGGCAAGGAACAGACGGTGGAAGCCGATGGCCTGCTCGCCACCTGCC
TGCAGCACGAGATCGACCATCTGAACGGCGTACTGTTCATCGATTATATCTCGCGGCTGAAGCGGGAGATGGTGATCAAG
AAGTTCACCAAGGCGGCAAAGTCCAAGGCGCTCTGA

Upstream 100 bases:

>100_bases
CGCGGCGAATATGGCGAAATTCCGGGCGGGGATGCAAAAAAGCGTCTTCCCCGCCTGCCGGATAATTCCATAGTCGGAAA
AGATGGGTTATGGGAACGCC

Downstream 100 bases:

>100_bases
GCATCGCATTGAAATTTCGTTGGCGAGGCATTATGATATCACTGATATCATAATGGAGGTGCCGATGGGTGACCTGCTGA
TCAGAAATATTTCCGAAGCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MTIKPLIILPDPILRQASKPIERVDADLQGLADDMLETMYDAPGIGLAAIQIGVPRRMLVIDVSREGEEKQPQVFINPEI
VKSSDERSVYEEGCLSIPDYYAEVERPAVVSVKFLDRNGKEQTVEADGLLATCLQHEIDHLNGVLFIDYISRLKREMVIK
KFTKAAKSKAL

Sequences:

>Translated_171_residues
MTIKPLIILPDPILRQASKPIERVDADLQGLADDMLETMYDAPGIGLAAIQIGVPRRMLVIDVSREGEEKQPQVFINPEI
VKSSDERSVYEEGCLSIPDYYAEVERPAVVSVKFLDRNGKEQTVEADGLLATCLQHEIDHLNGVLFIDYISRLKREMVIK
KFTKAAKSKAL
>Mature_170_residues
TIKPLIILPDPILRQASKPIERVDADLQGLADDMLETMYDAPGIGLAAIQIGVPRRMLVIDVSREGEEKQPQVFINPEIV
KSSDERSVYEEGCLSIPDYYAEVERPAVVSVKFLDRNGKEQTVEADGLLATCLQHEIDHLNGVLFIDYISRLKREMVIKK
FTKAAKSKAL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=156, Percent_Identity=35.8974358974359, Blast_Score=90, Evalue=8e-19,
Organism=Escherichia coli, GI1789682, Length=170, Percent_Identity=48.2352941176471, Blast_Score=156, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24645728, Length=178, Percent_Identity=31.4606741573034, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=30.188679245283, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19151; Mature: 19020

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKPLIILPDPILRQASKPIERVDADLQGLADDMLETMYDAPGIGLAAIQIGVPRRMLV
CCCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
IDVSREGEEKQPQVFINPEIVKSSDERSVYEEGCLSIPDYYAEVERPAVVSVKFLDRNGK
EECCCCCCCCCCEEEECHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCC
EQTVEADGLLATCLQHEIDHLNGVLFIDYISRLKREMVIKKFTKAAKSKAL
CCEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIKPLIILPDPILRQASKPIERVDADLQGLADDMLETMYDAPGIGLAAIQIGVPRRMLV
CCCCEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
IDVSREGEEKQPQVFINPEIVKSSDERSVYEEGCLSIPDYYAEVERPAVVSVKFLDRNGK
EECCCCCCCCCCEEEECHHHHCCCCHHHHHHHCCCCCCHHHHHCCCCEEEEEEEECCCCC
EQTVEADGLLATCLQHEIDHLNGVLFIDYISRLKREMVIKKFTKAAKSKAL
CCEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]