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The map label for this gene is thcR [H]

Identifier: 209547646

GI number: 209547646

Start: 35839

End: 36828

Strand: Direct

Name: thcR [H]

Synonym: Rleg2_0034

Alternate gene names: 209547646

Gene position: 35839-36828 (Clockwise)

Preceding gene: 209547645

Following gene: 209547652

Centisome position: 0.79

GC content: 59.9

Gene sequence:

>990_bases
ATGCCGGAAAATGCATCCGCCAATTTCAGCCCCCGATTTCAGGGGGCAACTTTCGACGACATGGTCGAGGCTCTTACCAA
GGGGTTCGGTTCGTTCGACGCATGGCGCCATGGTCGAGAGAAACCCCTCGACTGGAAGGTCGGGTTTTGGGGTGATGAGA
GGTTGTCGCTGGTCAGCAATCAGGATTCCGGCGGCTGGGGTGCAAGAACGGGACAGGGAGCGCCGGAAACTCTGGCCATC
GTCGTTCCGCGCACCGGTGCTCTCGACGTAACCTTTGGTCGGACTGTGGCCGAAGCAACACCCGGGCGCCTGTTGTTGAT
GAACAATATCGAGCCGGAACGGGTTTCCGTGAGGGCAGTCCCCCACCGCTCGGACACACTCAGTCTGAGCTGGACGATCC
TGGCGCAGACCGTCACCTCCGTCTTGGAGACCCCGCTGATCGGGTCATTGGACTTGGTGCCGGTCGTTGATCGTTCCACG
GCAGCGGGACAGTTGATCGGCAATCTCGCCCAAACGATGATCATCGGCATGCGCAACGCCGGCCCGCTGCTTCACTCGCC
GATTGCCATGCTGAACCTGATGCAGATGTTTGCCGATCTGGTGGTGCGCACGATTCCTCATCGGCTCTCGCATCTTCTCG
ACAAGAAAATCCACCTGATCGCGCCGCGGCACGTCCGTCGGGCAATTGAATTTATGCATGCGAACATCGCCCAGCCGTTG
ACAATGCAGAGTGTCGCGGAGGCGGCAGGAATTTCTATTCGCGCTCTGGAAGTCGGTTTTCGCGCCTTCAAGGGAACCAC
GCCGTCGGCCTACCTCAGGACGATCCGCCTGCAAGCGGTCCGGGAGGATCTGCGCGATCCTTCAAACCGGCAGCCGTTGC
GGGACATCTGTCTGAAGTGGGGATTCTTTCACCTCGGCCGTTTTGCGGCAACCTATCGGGCGGCGTATGGCGAGAACCCG
TCGGACACGCGAAGACGATTCGAGATATGA

Upstream 100 bases:

>100_bases
GGAAACGGTTGAAAGGGTTCATCCGATAAGTCATACTTTTGATGCGATAACCTTATACGCGGGGCATAATTTTAATATTT
CATCAGCCGTTTGGGTTCCC

Downstream 100 bases:

>100_bases
GGGCGCGCCATCGGCGAGCCTAAAGCGCGTCGCGATCTTTCAGTTCTAATGCATGTCGCCCGGAAGTGTGCAGGGGTTCC
GGGATAACGACATGCATCGA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MPENASANFSPRFQGATFDDMVEALTKGFGSFDAWRHGREKPLDWKVGFWGDERLSLVSNQDSGGWGARTGQGAPETLAI
VVPRTGALDVTFGRTVAEATPGRLLLMNNIEPERVSVRAVPHRSDTLSLSWTILAQTVTSVLETPLIGSLDLVPVVDRST
AAGQLIGNLAQTMIIGMRNAGPLLHSPIAMLNLMQMFADLVVRTIPHRLSHLLDKKIHLIAPRHVRRAIEFMHANIAQPL
TMQSVAEAAGISIRALEVGFRAFKGTTPSAYLRTIRLQAVREDLRDPSNRQPLRDICLKWGFFHLGRFAATYRAAYGENP
SDTRRRFEI

Sequences:

>Translated_329_residues
MPENASANFSPRFQGATFDDMVEALTKGFGSFDAWRHGREKPLDWKVGFWGDERLSLVSNQDSGGWGARTGQGAPETLAI
VVPRTGALDVTFGRTVAEATPGRLLLMNNIEPERVSVRAVPHRSDTLSLSWTILAQTVTSVLETPLIGSLDLVPVVDRST
AAGQLIGNLAQTMIIGMRNAGPLLHSPIAMLNLMQMFADLVVRTIPHRLSHLLDKKIHLIAPRHVRRAIEFMHANIAQPL
TMQSVAEAAGISIRALEVGFRAFKGTTPSAYLRTIRLQAVREDLRDPSNRQPLRDICLKWGFFHLGRFAATYRAAYGENP
SDTRRRFEI
>Mature_328_residues
PENASANFSPRFQGATFDDMVEALTKGFGSFDAWRHGREKPLDWKVGFWGDERLSLVSNQDSGGWGARTGQGAPETLAIV
VPRTGALDVTFGRTVAEATPGRLLLMNNIEPERVSVRAVPHRSDTLSLSWTILAQTVTSVLETPLIGSLDLVPVVDRSTA
AGQLIGNLAQTMIIGMRNAGPLLHSPIAMLNLMQMFADLVVRTIPHRLSHLLDKKIHLIAPRHVRRAIEFMHANIAQPLT
MQSVAEAAGISIRALEVGFRAFKGTTPSAYLRTIRLQAVREDLRDPSNRQPLRDICLKWGFFHLGRFAATYRAAYGENPS
DTRRRFEI

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=102, Percent_Identity=38.2352941176471, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 36397; Mature: 36266

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPENASANFSPRFQGATFDDMVEALTKGFGSFDAWRHGREKPLDWKVGFWGDERLSLVSN
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCCEEEECC
QDSGGWGARTGQGAPETLAIVVPRTGALDVTFGRTVAEATPGRLLLMNNIEPERVSVRAV
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHCCCCEEEEECCCCCCCEEEEEC
PHRSDTLSLSWTILAQTVTSVLETPLIGSLDLVPVVDRSTAAGQLIGNLAQTMIIGMRNA
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCC
GPLLHSPIAMLNLMQMFADLVVRTIPHRLSHLLDKKIHLIAPRHVRRAIEFMHANIAQPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHHHHHHHHHHHHHHCCCH
TMQSVAEAAGISIRALEVGFRAFKGTTPSAYLRTIRLQAVREDLRDPSNRQPLRDICLKW
HHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GFFHLGRFAATYRAAYGENPSDTRRRFEI
HHHHHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
PENASANFSPRFQGATFDDMVEALTKGFGSFDAWRHGREKPLDWKVGFWGDERLSLVSN
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCCCCEEEECC
QDSGGWGARTGQGAPETLAIVVPRTGALDVTFGRTVAEATPGRLLLMNNIEPERVSVRAV
CCCCCCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHCCCCEEEEECCCCCCCEEEEEC
PHRSDTLSLSWTILAQTVTSVLETPLIGSLDLVPVVDRSTAAGQLIGNLAQTMIIGMRNA
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCC
GPLLHSPIAMLNLMQMFADLVVRTIPHRLSHLLDKKIHLIAPRHVRRAIEFMHANIAQPL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEECCHHHHHHHHHHHHHHCCCH
TMQSVAEAAGISIRALEVGFRAFKGTTPSAYLRTIRLQAVREDLRDPSNRQPLRDICLKW
HHHHHHHHHCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GFFHLGRFAATYRAAYGENPSDTRRRFEI
HHHHHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]