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The map label for this gene is tme [H]

Identifier: 209547645

GI number: 209547645

Start: 33368

End: 35653

Strand: Direct

Name: tme [H]

Synonym: Rleg2_0033

Alternate gene names: 209547645

Gene position: 33368-35653 (Clockwise)

Preceding gene: 209547640

Following gene: 209547646

Centisome position: 0.74

GC content: 63.65

Gene sequence:

>2286_bases
ATGCCCGATATCGATAAGAAAGACCGGCCGGCGACATCAGTTACTGACCAGGAGGCGCTCGATTTTCACGCCACGGGCCG
GCCCGGCAAACTGGAAGTCGTCCCGACCAAGCCGATGGCGACGCAGCGCGACCTGTCGCTTGCCTATTCGCCGGGTGTCG
CCGTGCCCGTCAAGGCGATCGCCGCCGATCCGCAGACAGCTTACGATTACACCGCCCGCGGCAATATGGTTGCCGTCATT
TCCAACGGCACGGCGATCCTCGGTCTCGGCAATCTCGGCGCGCTGGCCTCCAAGCCCGTGATGGAAGGCAAGGCGGTGCT
GTTCAAGCGCTTCGCCGACGTCGATTCGATCGACCTCGAAATCGATACGGAAAATGTCGACGAGTTCATCAACTGCGTGC
GCTTCCTCGGCCCCTCCTTCGGCGGCATCAACCTCGAGGATATCAAAGCGCCGGATTGTTTCGTCATCGAAAGCCGGCTG
CGCGAGCTGATGGACATCCCGGTCTTCCACGACGACCAGCACGGAACGGCGATCATCGCCGCCGCCGGCCTGATCAACGC
GCTGGAACTGACCGGCCGCGACCTGAAGACGACCAAGCTTGTCTGCAACGGCGCCGGTGCTGCGGCGATCGCCTGTATCG
AGCTCATCAAGGCGATGGGCTTTGCGCCGGAAAATATCATCCTCTGCGACACCAAGGGCGTCATCTATCAGGGCCGCACC
GAGGGCATGAACCAGTGGAAATCGGCGCATGCGGCAAAGACCGATACACGCACGCTTGAGGAGGCGATGGACGGCGCTGA
CGTGGTTTTCGGCTTGTCGCAGAAGGGCGCCTTCTCGGCCGAGATGATCCGCTCGATGGCGGATCGGCCGATCATCTTCG
CCATGGCCAATCCCGATCCCGAAATCACCCCGGAAGAGGTGGCCCGCATCCGCGACGACGCCATCATGGCGACCGGCCGC
TCGGACTACCCGAACCAGGTCAACAACGTCCTCGGCTTTCCCTATATCTTCCGCGGCGCTCTCGACGTCCGCGCTTCCAC
CATCAACGACGCGATGAAGATCGCCGCGGTCAAGGCGCTGGCAAATCTTGCCCGCGAGGATGTGCCGGATGACGTGGTTG
CCGCCTATCAGGGCAACCGGCCGCGCTTCGGCTCGCAATACATCATCCCGGTTCCTTTCGATCCGCGGCTGATCTCGGCG
ATCCCGGTCGCGGTCGCGGAAGCGGCGATCGAAACCGGCGTCGCCCGCAAGGTCATCACCGACATGGCGGGTTATGCCCG
CGAGCTTTCGGCGCGACGCGATCCGATCGCCTCGACGCTTGCCAGCCTGTATGAGCGGGTGCGCCGCCGCCCGAAGCGGA
TCGTCTTTGCCGAGGCCGAGGAAGAACAGGTCCTGCGCGCAGCACTCTCTTACGCCAACCAGCAGCTCGGCACCGCCATT
CTGCTCGGCCGTGAGGACCTGATCCGATCGACGGCCGAACGCGCCGGCATCGATCTCAACCGGCCGGGGCTGGAAATCGT
CAATGCCCGTATTTCCACCCGGGTCGAGGCCTATATCGACTATCTCTATGCCAGGCTGCAGCGGCACGGTTATCTCCACC
GCGACGTCCAGCGGCTGATACACAACGACCGCAACCACTTCGCCGCCACTATGGTGGCGCTCGGCGATGCCGACGGCATG
GTGACGGGCATCACCCGCAACTATTCGACAGCACTTGAGGATGTGCGCCGCTGCATCGATGAAAAGCCTGGCCACCGCGT
CATCGGTGTCTCGTTGGCGCTCTGCCGCGGCCGCACGGTCTTCGTGGCCGATACCGCGGTGCATGACATGCCGACGGCCG
AGCAGCTTGCCGATATCGCCGAGGAGGCCGCCGGTCTGGCACGGCGCATGGGTTATCAGCCGCGCGTCGCCATGCTTGCC
TATTCCACCTTCGGCCATCCCTCCGGCGAACGCTCCGAGCGGGTGCGCGAGGCGGTAAAGATCCTCGACAAGCGCCGCGT
CGATTTCGAATATGACGGTGAAATGGCCGCCGATGTGGCGCTGAACCGCAAGGTGATGGAGCAATATCCCTTCTGCCGCC
TCTCGGGTCCCGCCAACGTGCTGGTCATGCCGGCTTTCCACTCGGCCTCGATCTCGACCAAGATGCTGCAGGAACTCGGC
GGCTCGACGGTGATCGGCCCGATCCTCGTCGGTCTCGACAAACCGGTGCAGATCACCTCGATGGGCGCCAAGGATAGCGA
CATCGTCAACATGGCGGCGATCGCCGCCTATGGCGCGGGTTCGTAA

Upstream 100 bases:

>100_bases
TTCCCAAAAAAACAGGTGCCAACCCTATCCGCGCGCCGCTATAGATGACAACAGCATTTGAGGAAGAGCGAAGCGTCGCC
CACAGGACGTTGGAGGATTT

Downstream 100 bases:

>100_bases
ACTCTCGGCCTTCCTATGAGACGGCATACCGTGCCTGGCCGACAGCACCTCGGCCGGGCACAGCTTTTTGTGGCTTACGC
ATCCAGGAAACGGTTGAAAG

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MPDIDKKDRPATSVTDQEALDFHATGRPGKLEVVPTKPMATQRDLSLAYSPGVAVPVKAIAADPQTAYDYTARGNMVAVI
SNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEIDTENVDEFINCVRFLGPSFGGINLEDIKAPDCFVIESRL
RELMDIPVFHDDQHGTAIIAAAGLINALELTGRDLKTTKLVCNGAGAAAIACIELIKAMGFAPENIILCDTKGVIYQGRT
EGMNQWKSAHAAKTDTRTLEEAMDGADVVFGLSQKGAFSAEMIRSMADRPIIFAMANPDPEITPEEVARIRDDAIMATGR
SDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAVKALANLAREDVPDDVVAAYQGNRPRFGSQYIIPVPFDPRLISA
IPVAVAEAAIETGVARKVITDMAGYARELSARRDPIASTLASLYERVRRRPKRIVFAEAEEEQVLRAALSYANQQLGTAI
LLGREDLIRSTAERAGIDLNRPGLEIVNARISTRVEAYIDYLYARLQRHGYLHRDVQRLIHNDRNHFAATMVALGDADGM
VTGITRNYSTALEDVRRCIDEKPGHRVIGVSLALCRGRTVFVADTAVHDMPTAEQLADIAEEAAGLARRMGYQPRVAMLA
YSTFGHPSGERSERVREAVKILDKRRVDFEYDGEMAADVALNRKVMEQYPFCRLSGPANVLVMPAFHSASISTKMLQELG
GSTVIGPILVGLDKPVQITSMGAKDSDIVNMAAIAAYGAGS

Sequences:

>Translated_761_residues
MPDIDKKDRPATSVTDQEALDFHATGRPGKLEVVPTKPMATQRDLSLAYSPGVAVPVKAIAADPQTAYDYTARGNMVAVI
SNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEIDTENVDEFINCVRFLGPSFGGINLEDIKAPDCFVIESRL
RELMDIPVFHDDQHGTAIIAAAGLINALELTGRDLKTTKLVCNGAGAAAIACIELIKAMGFAPENIILCDTKGVIYQGRT
EGMNQWKSAHAAKTDTRTLEEAMDGADVVFGLSQKGAFSAEMIRSMADRPIIFAMANPDPEITPEEVARIRDDAIMATGR
SDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAVKALANLAREDVPDDVVAAYQGNRPRFGSQYIIPVPFDPRLISA
IPVAVAEAAIETGVARKVITDMAGYARELSARRDPIASTLASLYERVRRRPKRIVFAEAEEEQVLRAALSYANQQLGTAI
LLGREDLIRSTAERAGIDLNRPGLEIVNARISTRVEAYIDYLYARLQRHGYLHRDVQRLIHNDRNHFAATMVALGDADGM
VTGITRNYSTALEDVRRCIDEKPGHRVIGVSLALCRGRTVFVADTAVHDMPTAEQLADIAEEAAGLARRMGYQPRVAMLA
YSTFGHPSGERSERVREAVKILDKRRVDFEYDGEMAADVALNRKVMEQYPFCRLSGPANVLVMPAFHSASISTKMLQELG
GSTVIGPILVGLDKPVQITSMGAKDSDIVNMAAIAAYGAGS
>Mature_760_residues
PDIDKKDRPATSVTDQEALDFHATGRPGKLEVVPTKPMATQRDLSLAYSPGVAVPVKAIAADPQTAYDYTARGNMVAVIS
NGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEIDTENVDEFINCVRFLGPSFGGINLEDIKAPDCFVIESRLR
ELMDIPVFHDDQHGTAIIAAAGLINALELTGRDLKTTKLVCNGAGAAAIACIELIKAMGFAPENIILCDTKGVIYQGRTE
GMNQWKSAHAAKTDTRTLEEAMDGADVVFGLSQKGAFSAEMIRSMADRPIIFAMANPDPEITPEEVARIRDDAIMATGRS
DYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAVKALANLAREDVPDDVVAAYQGNRPRFGSQYIIPVPFDPRLISAI
PVAVAEAAIETGVARKVITDMAGYARELSARRDPIASTLASLYERVRRRPKRIVFAEAEEEQVLRAALSYANQQLGTAIL
LGREDLIRSTAERAGIDLNRPGLEIVNARISTRVEAYIDYLYARLQRHGYLHRDVQRLIHNDRNHFAATMVALGDADGMV
TGITRNYSTALEDVRRCIDEKPGHRVIGVSLALCRGRTVFVADTAVHDMPTAEQLADIAEEAAGLARRMGYQPRVAMLAY
STFGHPSGERSERVREAVKILDKRRVDFEYDGEMAADVALNRKVMEQYPFCRLSGPANVLVMPAFHSASISTKMLQELGG
STVIGPILVGLDKPVQITSMGAKDSDIVNMAAIAAYGAGS

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=343, Percent_Identity=26.2390670553936, Blast_Score=89, Evalue=2e-17,
Organism=Homo sapiens, GI62420882, Length=359, Percent_Identity=26.4623955431755, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI62420880, Length=359, Percent_Identity=26.4623955431755, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI239049447, Length=359, Percent_Identity=26.4623955431755, Blast_Score=84, Evalue=5e-16,
Organism=Escherichia coli, GI1788806, Length=750, Percent_Identity=46.6666666666667, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=316, Percent_Identity=31.6455696202532, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788800, Length=325, Percent_Identity=32, Blast_Score=127, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17537199, Length=369, Percent_Identity=27.10027100271, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=269, Percent_Identity=30.4832713754647, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24646388, Length=350, Percent_Identity=30.8571428571429, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24646386, Length=350, Percent_Identity=30.8571428571429, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI281362674, Length=407, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21356279, Length=405, Percent_Identity=26.9135802469136, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI281362672, Length=407, Percent_Identity=27.027027027027, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI78707242, Length=488, Percent_Identity=25.2049180327869, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI78707238, Length=488, Percent_Identity=25.2049180327869, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI78707240, Length=397, Percent_Identity=26.1964735516373, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI78707232, Length=403, Percent_Identity=22.5806451612903, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363505, Length=403, Percent_Identity=22.5806451612903, Blast_Score=69, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78707236, Length=403, Percent_Identity=22.5806451612903, Blast_Score=69, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363503, Length=403, Percent_Identity=22.5806451612903, Blast_Score=69, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 82533; Mature: 82402

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDIDKKDRPATSVTDQEALDFHATGRPGKLEVVPTKPMATQRDLSLAYSPGVAVPVKAI
CCCCCCCCCCCCCCCCCHHHEEECCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHH
AADPQTAYDYTARGNMVAVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLE
CCCCCCCCCEECCCCEEEEEECCEEEEEECCCCHHHCCCCCCHHHHHHHHHCCCCCEEEE
IDTENVDEFINCVRFLGPSFGGINLEDIKAPDCFVIESRLRELMDIPVFHDDQHGTAIIA
ECHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEE
AAGLINALELTGRDLKTTKLVCNGAGAAAIACIELIKAMGFAPENIILCDTKGVIYQGRT
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCH
EGMNQWKSAHAAKTDTRTLEEAMDGADVVFGLSQKGAFSAEMIRSMADRPIIFAMANPDP
HHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCC
EITPEEVARIRDDAIMATGRSDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAVKAL
CCCHHHHHHHHHCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLAREDVPDDVVAAYQGNRPRFGSQYIIPVPFDPRLISAIPVAVAEAAIETGVARKVIT
HHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DMAGYARELSARRDPIASTLASLYERVRRRPKRIVFAEAEEEQVLRAALSYANQQLGTAI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEE
LLGREDLIRSTAERAGIDLNRPGLEIVNARISTRVEAYIDYLYARLQRHGYLHRDVQRLI
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HNDRNHFAATMVALGDADGMVTGITRNYSTALEDVRRCIDEKPGHRVIGVSLALCRGRTV
HCCCCHHEEHHEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEE
FVADTAVHDMPTAEQLADIAEEAAGLARRMGYQPRVAMLAYSTFGHPSGERSERVREAVK
EEECCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
ILDKRRVDFEYDGEMAADVALNRKVMEQYPFCRLSGPANVLVMPAFHSASISTKMLQELG
HHHHHCCCEEECCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCHHHHHHHHHCC
GSTVIGPILVGLDKPVQITSMGAKDSDIVNMAAIAAYGAGS
CCEEEHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PDIDKKDRPATSVTDQEALDFHATGRPGKLEVVPTKPMATQRDLSLAYSPGVAVPVKAI
CCCCCCCCCCCCCCCCHHHEEECCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHH
AADPQTAYDYTARGNMVAVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLE
CCCCCCCCCEECCCCEEEEEECCEEEEEECCCCHHHCCCCCCHHHHHHHHHCCCCCEEEE
IDTENVDEFINCVRFLGPSFGGINLEDIKAPDCFVIESRLRELMDIPVFHDDQHGTAIIA
ECHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCCEECCCCCCEEEE
AAGLINALELTGRDLKTTKLVCNGAGAAAIACIELIKAMGFAPENIILCDTKGVIYQGRT
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCH
EGMNQWKSAHAAKTDTRTLEEAMDGADVVFGLSQKGAFSAEMIRSMADRPIIFAMANPDP
HHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCC
EITPEEVARIRDDAIMATGRSDYPNQVNNVLGFPYIFRGALDVRASTINDAMKIAAVKAL
CCCHHHHHHHHHCCEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANLAREDVPDDVVAAYQGNRPRFGSQYIIPVPFDPRLISAIPVAVAEAAIETGVARKVIT
HHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DMAGYARELSARRDPIASTLASLYERVRRRPKRIVFAEAEEEQVLRAALSYANQQLGTAI
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEE
LLGREDLIRSTAERAGIDLNRPGLEIVNARISTRVEAYIDYLYARLQRHGYLHRDVQRLI
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
HNDRNHFAATMVALGDADGMVTGITRNYSTALEDVRRCIDEKPGHRVIGVSLALCRGRTV
HCCCCHHEEHHEEECCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEE
FVADTAVHDMPTAEQLADIAEEAAGLARRMGYQPRVAMLAYSTFGHPSGERSERVREAVK
EEECCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
ILDKRRVDFEYDGEMAADVALNRKVMEQYPFCRLSGPANVLVMPAFHSASISTKMLQELG
HHHHHCCCEEECCCHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCCCHHHHHHHHHCC
GSTVIGPILVGLDKPVQITSMGAKDSDIVNMAAIAAYGAGS
CCEEEHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]