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The map label for this gene is metK [H]

Identifier: 209547625

GI number: 209547625

Start: 11490

End: 12728

Strand: Reverse

Name: metK [H]

Synonym: Rleg2_0013

Alternate gene names: 209547625

Gene position: 12728-11490 (Counterclockwise)

Preceding gene: 209547626

Following gene: 209547624

Centisome position: 0.28

GC content: 63.2

Gene sequence:

>1239_bases
ATGCGCGCGAATTATCTCTTTACCAGCGAATCTGTTGCCGAAGGTCATCCTGACAAGGTCTGCGACCGCATCTCCGACGA
GATCGTCGATCTGGTCTACCGCGAAGCGGCCAAAACAGGCGTCAATCCGTGGGGCGTGCGCATTGCCTGCGAAACGCTGG
CGACCACCAACCGCGTCGTCATCGCCGGCGAAGTCCGCCTGCCGCCGAGCCTGATGAAGAAGGACAAGGACGGCAAGGAC
GTCATCAATCCGTCGAAGTTCAAGGCCGCCGCCCGCCGCGCCATCAAGGATATCGGTTATGAGCAGGACGGCTTCCACTG
GAAGAAGGCCAAGATCGACGTGCTCCTGCACTCGCAGTCGGCCGACATCGCGCAGGGCGTCGACAGTGCTGCCGACCAGC
AGGGCGACGAAGGCGCCGGCGACCAGGGCATCATGTTCGGTTACGCCTGCCGCGAAACGCCGGATCTGATGCCGGCGCCG
ATCTATTATTCCCACAAGATCCTGCAGCTGCTCGCGGTTGCCCGCAAGAAGGGTGACGGCGAAGTCGCCAAGCTCGGCCC
CGACGCCAAGAGCCAGGTGACCGTGCGCTACGTCGACGGCAAGCCGTCCGAAGCGACGTCGATCGTGCTTTCGACCCAGC
ATCTCGACGAAAGCTGGGATTCGAAGAAGGTCCGCGCCGTCGTCGAGCCCTATATCCGCGAAGCGCTCGGCGAACTGAAG
ATCGCTGACGATTGCAAGTGGTACATCAACCCGACCGGCAAGTTCGTCATCGGCGGACCGGACGGCGATGCGGGCCTCAC
CGGCCGCAAGATCATCGTCGACACCTACGGCGGTGCCGCTCCGCATGGCGGCGGCGCATTCTCCGGCAAGGACACGACGA
AGGTCGACCGTTCGGCTGCCTATGCCGCCCGCTATCTGGCGAAAAACGTCGTTGCTGCCGGCCTTGCCGACCGCTGCACG
ATCCAGATTTCCTACGCGATCGGCGTCGCCCAGCCGCTGTCGATCTATGTCGACCTGCACGGCACCGGCAAGGTCAGCGA
AGATCAGGTCGAAGCGGCTATCCGCAAGAACATGGACCTGTCTCCGTCGGGCATCCGCCGCCATCTCGACCTCAACAAGC
CGATCTATGCGAAGACCTCCGCTTATGGCCATTTCGGCCGCAAGGCCGGTCGCGACGGCTCGTTCTCCTGGGAGCGCACG
GACCTCGTCAAGGCGCTCAAGGAAAGCGTGAAGGCCTGA

Upstream 100 bases:

>100_bases
CCGCAGATATAAAGATATATTTATGTCCTTCTGCGCTTGTCATCGGGCCTCGAACTGAGTACCTACTAGCTAGCTTTTAT
TCTTTGAGGGGAATCCCGGA

Downstream 100 bases:

>100_bases
GATAAAGCATGACGGATATGGAACGCCGCGGCCGGGCGACTGAAGCCTTCTTCGGTCGCCGCAAGGGCAAGGCCCTGCGC
GAACACCAGGCCGAGACGCT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVNPWGVRIACETLATTNRVVIAGEVRLPPSLMKKDKDGKD
VINPSKFKAAARRAIKDIGYEQDGFHWKKAKIDVLLHSQSADIAQGVDSAADQQGDEGAGDQGIMFGYACRETPDLMPAP
IYYSHKILQLLAVARKKGDGEVAKLGPDAKSQVTVRYVDGKPSEATSIVLSTQHLDESWDSKKVRAVVEPYIREALGELK
IADDCKWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVAAGLADRCT
IQISYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKNMDLSPSGIRRHLDLNKPIYAKTSAYGHFGRKAGRDGSFSWERT
DLVKALKESVKA

Sequences:

>Translated_412_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVNPWGVRIACETLATTNRVVIAGEVRLPPSLMKKDKDGKD
VINPSKFKAAARRAIKDIGYEQDGFHWKKAKIDVLLHSQSADIAQGVDSAADQQGDEGAGDQGIMFGYACRETPDLMPAP
IYYSHKILQLLAVARKKGDGEVAKLGPDAKSQVTVRYVDGKPSEATSIVLSTQHLDESWDSKKVRAVVEPYIREALGELK
IADDCKWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVAAGLADRCT
IQISYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKNMDLSPSGIRRHLDLNKPIYAKTSAYGHFGRKAGRDGSFSWERT
DLVKALKESVKA
>Mature_412_residues
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVNPWGVRIACETLATTNRVVIAGEVRLPPSLMKKDKDGKD
VINPSKFKAAARRAIKDIGYEQDGFHWKKAKIDVLLHSQSADIAQGVDSAADQQGDEGAGDQGIMFGYACRETPDLMPAP
IYYSHKILQLLAVARKKGDGEVAKLGPDAKSQVTVRYVDGKPSEATSIVLSTQHLDESWDSKKVRAVVEPYIREALGELK
IADDCKWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAAYAARYLAKNVVAAGLADRCT
IQISYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKNMDLSPSGIRRHLDLNKPIYAKTSAYGHFGRKAGRDGSFSWERT
DLVKALKESVKA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=401, Percent_Identity=46.3840399002494, Blast_Score=313, Evalue=3e-85,
Organism=Homo sapiens, GI4557737, Length=400, Percent_Identity=46, Blast_Score=305, Evalue=5e-83,
Organism=Escherichia coli, GI1789311, Length=409, Percent_Identity=49.1442542787286, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17538498, Length=402, Percent_Identity=45.273631840796, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17538494, Length=402, Percent_Identity=45.0248756218905, Blast_Score=296, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI25145633, Length=404, Percent_Identity=43.8118811881188, Blast_Score=291, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI17551082, Length=415, Percent_Identity=42.6506024096386, Blast_Score=290, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI32565911, Length=320, Percent_Identity=47.5, Blast_Score=254, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI25145635, Length=319, Percent_Identity=46.0815047021944, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI32565905, Length=329, Percent_Identity=45.8966565349544, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=47.8547854785479, Blast_Score=239, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323209, Length=411, Percent_Identity=45.4987834549878, Blast_Score=313, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6320710, Length=402, Percent_Identity=44.5273631840796, Blast_Score=311, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24580527, Length=404, Percent_Identity=45.2970297029703, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI24580525, Length=404, Percent_Identity=45.2970297029703, Blast_Score=295, Evalue=5e-80,
Organism=Drosophila melanogaster, GI45552159, Length=404, Percent_Identity=44.8019801980198, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24580529, Length=404, Percent_Identity=44.8019801980198, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24580537, Length=404, Percent_Identity=44.8019801980198, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24580533, Length=404, Percent_Identity=44.8019801980198, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24580535, Length=404, Percent_Identity=44.8019801980198, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24580531, Length=404, Percent_Identity=44.8019801980198, Blast_Score=287, Evalue=8e-78,
Organism=Drosophila melanogaster, GI24580541, Length=388, Percent_Identity=44.3298969072165, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24580539, Length=142, Percent_Identity=36.6197183098592, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44582; Mature: 44582

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVNPWGVRIACETLATTNRVV
CCCCEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEE
IAGEVRLPPSLMKKDKDGKDVINPSKFKAAARRAIKDIGYEQDGFHWKKAKIDVLLHSQS
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCC
ADIAQGVDSAADQQGDEGAGDQGIMFGYACRETPDLMPAPIYYSHKILQLLAVARKKGDG
CHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EVAKLGPDAKSQVTVRYVDGKPSEATSIVLSTQHLDESWDSKKVRAVVEPYIREALGELK
CEEECCCCCCCEEEEEEECCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCE
IADDCKWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAA
ECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
YAARYLAKNVVAAGLADRCTIQISYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKNMDL
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC
SPSGIRRHLDLNKPIYAKTSAYGHFGRKAGRDGSFSWERTDLVKALKESVKA
CHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRANYLFTSESVAEGHPDKVCDRISDEIVDLVYREAAKTGVNPWGVRIACETLATTNRVV
CCCCEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEE
IAGEVRLPPSLMKKDKDGKDVINPSKFKAAARRAIKDIGYEQDGFHWKKAKIDVLLHSQS
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCC
ADIAQGVDSAADQQGDEGAGDQGIMFGYACRETPDLMPAPIYYSHKILQLLAVARKKGDG
CHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EVAKLGPDAKSQVTVRYVDGKPSEATSIVLSTQHLDESWDSKKVRAVVEPYIREALGELK
CEEECCCCCCCEEEEEEECCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCE
IADDCKWYINPTGKFVIGGPDGDAGLTGRKIIVDTYGGAAPHGGGAFSGKDTTKVDRSAA
ECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHH
YAARYLAKNVVAAGLADRCTIQISYAIGVAQPLSIYVDLHGTGKVSEDQVEAAIRKNMDL
HHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC
SPSGIRRHLDLNKPIYAKTSAYGHFGRKAGRDGSFSWERTDLVKALKESVKA
CHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA