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The map label for this gene is grpE [H]

Identifier: 209547620

GI number: 209547620

Start: 7808

End: 8440

Strand: Direct

Name: grpE [H]

Synonym: Rleg2_0008

Alternate gene names: 209547620

Gene position: 7808-8440 (Clockwise)

Preceding gene: 209547619

Following gene: 209547622

Centisome position: 0.17

GC content: 64.93

Gene sequence:

>633_bases
ATGACCGATGACACGACGAAAAACGGACCTGACGCAACTGCGGCCGATGCCGCAGCCGACGCTGCCGCCTACGTCGAGAA
CGATATTGCGCAGGAAGAGGCCCCCCAGCCTGATGCACTCGAGCTTCTGAAAGCCGAAAACGGCGAACTGCGTGACCGCT
ATCTGCGCCTTGCCGCCGAGATGGACAATCTGCGCCGCCGCACCGAGCGTGAGGTCAAGGACGCCAAGTCCTATTCCGTC
GCCGGTTTCGCGCGTGACATGCTCGCCGTCTCGGACAATTTGCGCCGGGCGCTGGATGCCATCCCGGCAGAGGTCAAGGA
TGCGGCCGATGCCGGCCTGAGCACGCTGATCGAGGGCGTCGAGATGACCGAGCGCGCCATGCTGTCGGCGCTCGAGCGCC
ACGGCGTTCGCAAGCTGGAGCCGGTCGGCCAGAAATTCGACCCGAATTTCCATCAGGCGATGTTCGAGGTGCCGAACCCG
GATGTGCCGAACAACACGGTCGTCCAGGTCGTGCAGGCAGGCTTTTCCATCGGCGAGCGCGTGCTGCGCCCGGCCATGGT
CGGCGTCGCCAAGGGCGGCCCGAAGGCCGCCGAAGCCGAAACCAATTCGGTCTTCGACGAGAAGGACGCCTGA

Upstream 100 bases:

>100_bases
CGAAGACCGCGGAATTTTCGCTTCTTTGTCACGCAGACTTGATTTTTTTCGGTCAAACCTCGATATCGGGCGCATCTGAA
GACACCAACCGGAGACCGTC

Downstream 100 bases:

>100_bases
AACCTTCTCCCTTGGGAGAAGGGTGCCGAAATCAGATGCGGGCGAAGCGTTCGATCAACAGATCGAAGAAGCCGTCCGCA
TCGACTTGGCGCATCACTTT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MTDDTTKNGPDATAADAAADAAAYVENDIAQEEAPQPDALELLKAENGELRDRYLRLAAEMDNLRRRTEREVKDAKSYSV
AGFARDMLAVSDNLRRALDAIPAEVKDAADAGLSTLIEGVEMTERAMLSALERHGVRKLEPVGQKFDPNFHQAMFEVPNP
DVPNNTVVQVVQAGFSIGERVLRPAMVGVAKGGPKAAEAETNSVFDEKDA

Sequences:

>Translated_210_residues
MTDDTTKNGPDATAADAAADAAAYVENDIAQEEAPQPDALELLKAENGELRDRYLRLAAEMDNLRRRTEREVKDAKSYSV
AGFARDMLAVSDNLRRALDAIPAEVKDAADAGLSTLIEGVEMTERAMLSALERHGVRKLEPVGQKFDPNFHQAMFEVPNP
DVPNNTVVQVVQAGFSIGERVLRPAMVGVAKGGPKAAEAETNSVFDEKDA
>Mature_209_residues
TDDTTKNGPDATAADAAADAAAYVENDIAQEEAPQPDALELLKAENGELRDRYLRLAAEMDNLRRRTEREVKDAKSYSVA
GFARDMLAVSDNLRRALDAIPAEVKDAADAGLSTLIEGVEMTERAMLSALERHGVRKLEPVGQKFDPNFHQAMFEVPNPD
VPNNTVVQVVQAGFSIGERVLRPAMVGVAKGGPKAAEAETNSVFDEKDA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=140, Percent_Identity=39.2857142857143, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI40255109, Length=150, Percent_Identity=34.6666666666667, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1788967, Length=142, Percent_Identity=39.4366197183099, Blast_Score=106, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17552458, Length=150, Percent_Identity=37.3333333333333, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324806, Length=147, Percent_Identity=42.1768707482993, Blast_Score=126, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24653432, Length=148, Percent_Identity=41.8918918918919, Blast_Score=136, Evalue=1e-32,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22695; Mature: 22563

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDTTKNGPDATAADAAADAAAYVENDIAQEEAPQPDALELLKAENGELRDRYLRLAAE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHH
MDNLRRRTEREVKDAKSYSVAGFARDMLAVSDNLRRALDAIPAEVKDAADAGLSTLIEGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EMTERAMLSALERHGVRKLEPVGQKFDPNFHQAMFEVPNPDVPNNTVVQVVQAGFSIGER
HHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VLRPAMVGVAKGGPKAAEAETNSVFDEKDA
HHHHHHHHHCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
TDDTTKNGPDATAADAAADAAAYVENDIAQEEAPQPDALELLKAENGELRDRYLRLAAE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHH
MDNLRRRTEREVKDAKSYSVAGFARDMLAVSDNLRRALDAIPAEVKDAADAGLSTLIEGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EMTERAMLSALERHGVRKLEPVGQKFDPNFHQAMFEVPNPDVPNNTVVQVVQAGFSIGER
HHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VLRPAMVGVAKGGPKAAEAETNSVFDEKDA
HHHHHHHHHCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA