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The map label for this gene is sfmD [H]

Identifier: 209401002

GI number: 209401002

Start: 655425

End: 658034

Strand: Direct

Name: sfmD [H]

Synonym: ECH74115_0633

Alternate gene names: 209401002

Gene position: 655425-658034 (Clockwise)

Preceding gene: 209397289

Following gene: 209395712

Centisome position: 11.76

GC content: 47.7

Gene sequence:

>2610_bases
ATGAAAATACCCACTACTACGGATATTCCGCAGAGGTATACCTGGTGTCTGGCCGGAATTTGTTATTCATCTCTTGCCAT
TTTACCCTCCTTTTTAAGCTATGCGGAAAGTTATTTCAACCCGGCATTTTTATTAGAGAATGGCACATTCGTTGCTGATT
TATCGCGCTTTGAGAGAGGTAATCATCAACCTGCGGGCGTGTATCGGGTGGATCTCTGGCGTAATGATGAGTTCATTGGT
TCACAGGATATCGTATTTGAATCGACAACAGAAAATACAGGTGATAAATCAGGTGGGTTAATGCCCTGTTTTAACCAGGT
ACTCCTTGAACGAATTGGCCTTAATAGCAGTGCATTTCCCGAGTTAGCCCAGCAGCAAAACAATAAATGCATCAATTTAC
TGAAAGCTGTACCTGATGCCACAATTAACTTTGATTTTGCAGCGATGCGCCTGAACATCACTATTCCTCAGATAGCGTTG
TTGAGTAGCGCTCACGGTTACATTCCGCCTGAAGAGTGGGATGAAGGTATTCCTGCTTTACTCCTGAATTATAATTTCAC
CGGTAACAGAGGTAATGGTAACGATAGCTATTTTTTTAGTGAGCTCAGCGGGATTAATATTGGCCCGTGGCGTTTACGCA
ACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGC
GCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGTGTTGGATTTCG
TGGTGTACGGCTTTATTCTTCCGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCC
GTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATCTATCAAAGCTATGTTTCCCCCGGCGCTTTTGAAATT
ACAGATTTGCACCCGACATCTTCAAATGGCGATCTGGACGTCACCATCGACGAACGCGATGGCAATCAGCAGAATTACAC
TATTCCGTATTCAACAGTGCCAATTTTACAACGCGAAGGGCGTTTCAAATTTGACCTGACGGCGGGCGATTTTCGTAGCG
GTAATAGTCAGCAATCATCGCCTTTCTTTTTTCAGGGCACGGCACTCGGCGGTTTACCACAGGAATTTACTGCCTACGGC
GGGACGCAATTATCTGCCAATTACACCGCCTTTTTATTAGGGCTGGGGCGCAACCTCGGGAACTGGGGCGCAGTGTCGCT
GGATGTGACCCATGCGCGCAGTCAGTTAGCCGACGACAGTCGTCATGAGGGGGATTCCATTCGCTTCCTCTATGCGAAAT
CGATGAACACCTTCGGCACCAATTTTCAGTTAATGGGTTACCGCTATTCGACACAAGGTTTTTATACCCTTGATGATGTT
GCGTATCGTCGAATGGAGGGGTACGAATATGATTACGATTATGACGGTGAGCATCGGGATGAACCGATAATCGTGAATTA
CCACAATTTACGCTTTAGCCGTAAAGACCGTTTGCAGTTAAATATTTCACAATCACTTAATGACTTTGGCTCGCTTTATA
TCTCTGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACCTGGTATCAGGTGGGGTATACCAGCAGCTGGGTT
GGCATCAGTTATTCACTCTCATTTTCGTGGAATGAATCTGTAGGGATCCCCGATAACGAACGTATTGTCGGACTTAATGT
TTCAGTGCCTTTCAATGTTCTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACG
CCAACCGTAACAGCAACGGGCAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTAT
CACGTAAGCCAGGGTGATACCTCGAATAATGGGTATACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGGTACGCT
GGGGGTCGGGTATAACTACGACCGCGATCAACATGACGTTAACTGGCAGCTGTCTGGCGGTGTGGTCGGACATGAAAATG
GCATAACGCTGAGCCAGCCTTTAGGGGATACCAATGTTTTGATTAAAGCGCCTGGCGCAGGCGGTGTACGCATTGAAAAT
CAAACTGGCATTTTAACCGACTGGCGCGGCTATGCGGTGATGCCGTATGCCACGGTTTATCGGTATAACCGTATCGCGCT
TGATACCAATACGATGGGGAATTCCATCGATGTTGAAAAAAATATTAGCAGCGTTGTGCCGACGCAAGGCGCGTTGGTTC
GTGCCAATTTTGATACCCGCATAGGCGTGCGGGCGCTCATTACCGTTACCCAGGGCGGAAAACCGGTGCCGTTTGGATCA
CTGGTACGGGAAAACAGTACCGGAATAACCAGTATGGTGGGTGATGACGGGCAAGTTTATTTAAGTGGTGCGCCATTGTC
TGGTGAATTACTGGTTCAGTGGGGAGACGGCGCGAACTCACGCTGCATTGCGCACTATGTATTGCCGAAGCAAAGCTTAC
AGCAAGCCGTCACTGTTATTTCGGCAGTTTGCACACATCCTGGCTCATAA

Upstream 100 bases:

>100_bases
AAAGCTGAAATTTCAGACCGTTAATGATTATGGTTCAGTAACCCCGGTCAGAGAAGTGAACTTAAACTAACCGAATCATC
TGACAATATCAGAGCTAATT

Downstream 100 bases:

>100_bases
AGGAAATTATCAATGAAGATAATCTGCAGATTATTATTGGCGATGGCATGTTTGTGTCTGGCAAACATATCCTGGGCTAC
TGTTTGTGCAAATAGTACTG

Product: outer membrane usher protein SfmD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 869; Mature: 869

Protein sequence:

>869_residues
MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFLLENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIG
SQDIVFESTTENTGDKSGGLMPCFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSWNYFRGNGYHSEQWNNIGTWVQR
AIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDNMYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEI
TDLHPTSSNGDLDVTIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGLPQEFTAYG
GTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDV
AYRRMEGYEYDYDYDGEHRDEPIIVNYHNLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWV
GISYSLSFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSWLAGVGGTLLEGHNLSY
HVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNWQLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIEN
QTGILTDWRGYAVMPYATVYRYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPFGS
LVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQAVTVISAVCTHPGS

Sequences:

>Translated_869_residues
MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFLLENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIG
SQDIVFESTTENTGDKSGGLMPCFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSWNYFRGNGYHSEQWNNIGTWVQR
AIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDNMYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEI
TDLHPTSSNGDLDVTIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGLPQEFTAYG
GTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDV
AYRRMEGYEYDYDYDGEHRDEPIIVNYHNLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWV
GISYSLSFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSWLAGVGGTLLEGHNLSY
HVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNWQLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIEN
QTGILTDWRGYAVMPYATVYRYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPFGS
LVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQAVTVISAVCTHPGS
>Mature_869_residues
MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFLLENGTFVADLSRFERGNHQPAGVYRVDLWRNDEFIG
SQDIVFESTTENTGDKSGGLMPCFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSWNYFRGNGYHSEQWNNIGTWVQR
AIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDNMYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEI
TDLHPTSSNGDLDVTIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGLPQEFTAYG
GTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDV
AYRRMEGYEYDYDYDGEHRDEPIIVNYHNLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWV
GISYSLSFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSWLAGVGGTLLEGHNLSY
HVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNWQLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIEN
QTGILTDWRGYAVMPYATVYRYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPFGS
LVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQAVTVISAVCTHPGS

Specific function: Involved in the export and assembly of sfmA fimbrial subunits across the outer membrane [H]

COG id: COG3188

COG function: function code NU; P pilus assembly protein, porin PapC

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fimbrial export usher family [H]

Homologues:

Organism=Escherichia coli, GI1786744, Length=869, Percent_Identity=99.1944764096663, Blast_Score=1762, Evalue=0.0,
Organism=Escherichia coli, GI1790772, Length=878, Percent_Identity=46.9248291571754, Blast_Score=802, Evalue=0.0,
Organism=Escherichia coli, GI1787172, Length=852, Percent_Identity=45.18779342723, Blast_Score=751, Evalue=0.0,
Organism=Escherichia coli, GI1789533, Length=860, Percent_Identity=37.6744186046512, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1786332, Length=873, Percent_Identity=31.1569301260023, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1788427, Length=831, Percent_Identity=29.8435619735259, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI87081778, Length=787, Percent_Identity=27.9542566709022, Blast_Score=253, Evalue=5e-68,
Organism=Escherichia coli, GI1789610, Length=776, Percent_Identity=25.2577319587629, Blast_Score=144, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000015
- InterPro:   IPR018030 [H]

Pfam domain/function: PF00577 Usher [H]

EC number: NA

Molecular weight: Translated: 96100; Mature: 96100

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01151 FIMBRIAL_USHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFLLENGTFVADLSRFERG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHCC
NHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMPCFNQVLLERIGLNSSAFP
CCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCH
ELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIALLSSAHGYIPPEEWDEGIPAL
HHHHHCCCHHHHHHHHCCCCEEEEEEEEEEEEEECCCEEEECCCCCCCCCHHHHCCCCEE
LLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSWNYFRGNGYHSEQWNNIGTWVQR
EEEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCCCCHHHHHHHH
AIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDNMYPDSQQGFAPTVRGIARTAAQLT
HHCCCCCEEEEECCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHEEEE
IRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDVTIDERDGNQQNYTIPYSTVPILQREG
EECCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCEECCC
RFKFDLTAGDFRSGNSQQSSPFFFQGTALGGLPQEFTAYGGTQLSANYTAFLLGLGRNLG
CEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCEEECCCCEEEEEECCCCCC
NWGAVSLDVTHARSQLADDSRHEGDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDV
CCCEEEEEEHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCEEHHHH
AYRRMEGYEYDYDYDGEHRDEPIIVNYHNLRFSRKDRLQLNISQSLNDFGSLYISGTHQK
HHHHHCCCCCCCCCCCCCCCCCEEEEEECEEECCCCCEEEEHHHCCCCCCEEEEEECCHH
YWNTSDSDTWYQVGYTSSWVGISYSLSFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYT
CCCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
RENALDRAYASFNANRNSNGQNSWLAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANW
HHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEECCCCEEEEECCCCCCCCEECCCCCCC
QAAYGTLGVGYNYDRDQHDVNWQLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIEN
EEECEEEECCCCCCCCCCCCCEEECCCEEECCCCEEEECCCCCCEEEEECCCCCCEEEEC
QTGILTDWRGYAVMPYATVYRYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTR
CCCEEECCCCEEECCCHHHEEEEEEEEECCCCCCEEECCCCHHHHCCCCCEEEEECCCCC
IGVRALITVTQGGKPVPFGSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANS
CCEEEEEEEECCCCCCCHHHHHCCCCCCCEEEECCCCEEEEECCCCCCEEEEEECCCCCC
RCIAHYVLPKQSLQQAVTVISAVCTHPGS
CEEEEEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFLLENGTFVADLSRFERG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEHHHHHCC
NHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMPCFNQVLLERIGLNSSAFP
CCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCH
ELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIALLSSAHGYIPPEEWDEGIPAL
HHHHHCCCHHHHHHHHCCCCEEEEEEEEEEEEEECCCEEEECCCCCCCCCHHHHCCCCEE
LLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSWNYFRGNGYHSEQWNNIGTWVQR
EEEEEECCCCCCCCCCEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCCCCHHHHHHHH
AIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDNMYPDSQQGFAPTVRGIARTAAQLT
HHCCCCCEEEEECCCCCCHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHEEEE
IRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDVTIDERDGNQQNYTIPYSTVPILQREG
EECCCEEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCEECCC
RFKFDLTAGDFRSGNSQQSSPFFFQGTALGGLPQEFTAYGGTQLSANYTAFLLGLGRNLG
CEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCEEECCCCEEEEEECCCCCC
NWGAVSLDVTHARSQLADDSRHEGDSIRFLYAKSMNTFGTNFQLMGYRYSTQGFYTLDDV
CCCEEEEEEHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEEECCCCCEEHHHH
AYRRMEGYEYDYDYDGEHRDEPIIVNYHNLRFSRKDRLQLNISQSLNDFGSLYISGTHQK
HHHHHCCCCCCCCCCCCCCCCCEEEEEECEEECCCCCEEEEHHHCCCCCCEEEEEECCHH
YWNTSDSDTWYQVGYTSSWVGISYSLSFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYT
CCCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHH
RENALDRAYASFNANRNSNGQNSWLAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANW
HHHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEECCCCEEEEECCCCCCCCEECCCCCCC
QAAYGTLGVGYNYDRDQHDVNWQLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIEN
EEECEEEECCCCCCCCCCCCCEEECCCEEECCCCEEEECCCCCCEEEEECCCCCCEEEEC
QTGILTDWRGYAVMPYATVYRYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTR
CCCEEECCCCEEECCCHHHEEEEEEEEECCCCCCEEECCCCHHHHCCCCCEEEEECCCCC
IGVRALITVTQGGKPVPFGSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANS
CCEEEEEEEECCCCCCCHHHHHCCCCCCCEEEECCCCEEEEECCCCCCEEEEEECCCCCC
RCIAHYVLPKQSLQQAVTVISAVCTHPGS
CEEEEEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]