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The map label for this gene is ynfE [H]

Identifier: 209400979

GI number: 209400979

Start: 2164166

End: 2166592

Strand: Direct

Name: ynfE [H]

Synonym: ECH74115_2296

Alternate gene names: 209400979

Gene position: 2164166-2166592 (Clockwise)

Preceding gene: 209400471

Following gene: 209396602

Centisome position: 38.84

GC content: 50.76

Gene sequence:

>2427_bases
ATGTCCAAAAATGAACGAATGGTGGGCATCAGCCGCAGAACACTCGTTAAATCTACCGCGATAGGTTCTCTGGCGCTGGC
TGCAGGCGGTTTTTCTTTGCCGTTCACCCTGCGCAGTGCAGCGGCAGCAGTACAACAGGCCAGCGAACAAGTGGTCTGGG
GTGCCTGTTCCGTCAACTGTGGTAGCCGCTGTGCACTTCGTCTACATGTTAAAGATAATGAAGTGGCCTGGGTGGAAACT
GACAACACCGGCAGCGATGAGTATGGCAACCATCAGGTACGCGCCTGTTTGCGCGGTCGCTCCATCCGCCGGCGTATTAA
TCATCCCGATCGCTTGAATTACCCAATGAAACGCGTGGGTAAACGCGGTGAAGGCAAATTCGAACGGATTAGCTGGGATG
AAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCT
TCGGGGATCGTTGGCGGCAATATAACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGG
TTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCA
ACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCGGCGGT
GGCATCACTTATCTTCTTGAAAAATCCCGCGAAAAATCGAACGCAAAAATGATTGTTATCGATCCGCGATATACCGATAC
GGCTGCTGGTCGTGAAGATGAATGGCTTCCTATTCGCCCGGGCACCGATGCCGCGCTGGTTGCGGGTATTGCCTGGGTAT
TGATTAACGAAAATCTCGTTGATCAACCTTTTCTCGATAAATACTGCGTCGGCTATGACGAAAAAACCTTACCCGCAGAT
GCACCCAAAAATGGTCACTATAAAGCCTATATTCTTGGTGAAGGTGACGATAAAACAGCGAAAACGCCGCAGTGGGCTTC
GCAAATTACCGGTATCCCGGTGGACCGTATCATCAAACTGGCGCGTGAAATTGGCACAGCAAAACCCGCTTATATCTGCC
AGGGCTGGGGGCCGCAACGCCAGGCAAACGGCGAGCTGACCGCACGCGCTATTGCTATGTTGCCTATTCTGACGGGCAAT
GTCGGCATCAGCGGCGGAAATAGTGGCGCGCGTGAATCGACCTATACCATTACCATAGAGCGCCTGCCGGTGCTGGATAA
TCCGGTCAAAACGTCAATTTCCTGCTTCAGCTGGACAGATGCTATCGATCATGGCCCACAAATGACGGCAATCCGCGACG
GTGTCCGGGGCAAAGATAAACTGGATGTGCCCATTAAGTTCATCTGGAACTACGCGGGAAATACCCTGGTTAATCAGCAT
TCTGACATCAACAAAACGCATGAAATTCTGCAGGACGAATCGAAATGCGAAATGATTGTGGTCATCGAAAACTTTATGAC
CTCATCGGCAAAATATGCCGACATTCTGCTGCCAGACCTGATGACTGTTGAGCAGGAAGATATTATTCCTAACGACTACG
CCGGTAACATGGGATATCTCATTTTCCTCCAGCCTGTCACCAGCGAAAAATTCGAACGCAAACCGATTTACTGGATCCTG
AGTGAAGTCGCGAAACGTCTTGGACCTGACGTCTATCAAAAGTTCACTGAAGGTCGCACGCAGGAACAATGGTTACAACA
TCTGTACGCCAAAATGCTTGCCAAAGATCCGGCGTTACCGTCTTACGACGAACTGAAAAAAATGGGTATCTATAAGCGTA
AAGATCCCAATGGTCATTTTGTCGCCTACAAAGCATTTCGTGACGACCCCGAGGCAAATCCACTTAAAACACCTTCCGGT
AAGATTGAAATTTATTCCAGCAAGCTGGCGGAAATTGCCCGTACCTGGGAACTGGAAAAAGATGAAGTGATAAGCCCACT
GCCAGTTTATGCCTCAACCTTTGAAGGCTGGGACTCCCCTGAACGTAGTACCTTTCCACTGCAACTGTTTGGTTTCCATT
ACAAATCCCGTACTCACTCGACCTACGGCAACATTGATGTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCT
ATAGACGCACAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTATAACCATCGTGGCGAAGTTCGGCTACCAGC
CAAAGTGACACCACGCATTCTCCCTGGGGTTAGCGCTATGGGCCAGGGAGCCTGGCACGAGGCCAATATGTCTGGCGATA
AAATCGACCATGGCGGATGTGTGAATACGCTAACCACTCTGCGCCCTTCACCACTGGCGAAGGGAAACCCGCAGCACACG
AATCTGGTCGAGATCGAAAAAATATAA

Upstream 100 bases:

>100_bases
ATAATTATTAACCCAAAATGGGTAGACTCCCTCTATTGTTAGCGCGCTAAATATTCAATATATAAACTTTTATATAACGA
TAAAGAACAGGGAGTGAGTT

Downstream 100 bases:

>100_bases
CCCACTACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAATCCATACCACAGA
GGCGCTCATGAAGGCTGAAA

Product: anaerobic dimethyl sulfoxide reductase, A subunit YnfE

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 808; Mature: 807

Protein sequence:

>808_residues
MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVET
DNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI

Sequences:

>Translated_808_residues
MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVET
DNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
SGIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
GITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPAD
APKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGN
VGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWIL
SEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
KIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
NLVEIEKI
>Mature_807_residues
SKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEQVVWGACSVNCGSRCALRLHVKDNEVAWVETD
NTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSS
GIVGGNITRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGGG
ITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADA
PKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNV
GISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQHS
DINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILS
EVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGK
IEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPI
DAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTN
LVEIEKI

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787870, Length=808, Percent_Identity=98.5148514851485, Blast_Score=1664, Evalue=0.0,
Organism=Escherichia coli, GI171474008, Length=801, Percent_Identity=70.6616729088639, Blast_Score=1228, Evalue=0.0,
Organism=Escherichia coli, GI87081797, Length=805, Percent_Identity=66.832298136646, Blast_Score=1134, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=753, Percent_Identity=30.2788844621514, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI87081994, Length=848, Percent_Identity=28.5377358490566, Blast_Score=262, Evalue=7e-71,
Organism=Escherichia coli, GI1787231, Length=760, Percent_Identity=29.2105263157895, Blast_Score=262, Evalue=7e-71,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=24.2118537200504, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI3868720, Length=457, Percent_Identity=25.382932166302, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788534, Length=890, Percent_Identity=22.6966292134831, Blast_Score=84, Evalue=5e-17,
Organism=Escherichia coli, GI3868719, Length=457, Percent_Identity=24.507658643326, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787741, Length=427, Percent_Identity=25.2927400468384, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 89529; Mature: 89397

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEQVVWGACSVNC
CCCCCCEEECHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNITRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEEECCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAVQQASEQVVWGACSVNC
CCCCCEEECHHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCC
GSRCALRLHVKDNEVAWVETDNTGSDEYGNHQVRACLRGRSIRRRINHPDRLNYPMKRVG
CCEEEEEEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHC
KRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYSSGIVGGNITRSSPSASAVKR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCHHHHHH
LMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTDIENSKLVVMFGNNPAETRMSGG
HHHHHCCCCHHCCCCCCEEEEEEECEEECCCCCCCCCCCCCCEEEEEECCCCHHCEECCC
GITYLLEKSREKSNAKMIVIDPRYTDTAAGREDEWLPIRPGTDAALVAGIAWVLINENLV
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEEECCCCC
DQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGEGDDKTAKTPQWASQITGIPVDRIIKL
CCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHHH
AREIGTAKPAYICQGWGPQRQANGELTARAIAMLPILTGNVGISGGNSGARESTYTITIE
HHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHEEECCEEECCCCCCCCCCEEEEEEE
RLPVLDNPVKTSISCFSWTDAIDHGPQMTAIRDGVRGKDKLDVPIKFIWNYAGNTLVNQH
ECCCCCCCHHCCEEEEEEHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECCCCCEECCC
SDINKTHEILQDESKCEMIVVIENFMTSSAKYADILLPDLMTVEQEDIIPNDYAGNMGYL
CCCHHHHHHHCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCEEE
IFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALP
EEEECCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
SYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEK
CHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCH
DEVISPLPVYASTFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINP
HHCCCCCCHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
IDAQKRGIANGDMVRVYNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGC
CCHHHCCCCCCCEEEEEECCCCEEECCCCCCHHCCCCHHHCCCCCEECCCCCCCCCCCCH
VNTLTTLRPSPLAKGNPQHTNLVEIEKI
HHHHHHCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]