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The map label for this gene is ynfF [H]

Identifier: 209396602

GI number: 209396602

Start: 2166680

End: 2169076

Strand: Direct

Name: ynfF [H]

Synonym: ECH74115_2297

Alternate gene names: 209396602

Gene position: 2166680-2169076 (Clockwise)

Preceding gene: 209400979

Following gene: 209399816

Centisome position: 38.88

GC content: 51.11

Gene sequence:

>2397_bases
ATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTGCTTTCAC
TCTGCCATTTTCCCAAATGGTCCGAGCGGCAGAGGCACCGGTAGAAGAGAAAGCGGTCTGGAGTTCCTGCACCGTTAACT
GCGGGAGCCGCTGTCTGTTACGTTTGCATGTGAAAGATGACACCGTGTACTGGGTGGAGTCTGATACGACAGGTGACGAC
GTCTACGGTAATCATCAGGTTCGTGCGTGTTTACGCGGGCGCTCTATCCGCCGACGGATGAATCATCCTGATAGGTTGAA
ATATCCCATGAAGCGCGTCGGCAAGCGCGGTGAAGGTAAATTTGAACGGATAAGTTGGGACGAAGCCCTGGATACCATCA
GTGATAATCTTCGGCGGATCCTGAAAGATTACGGCAATGAGGCTGTACATGTCCTGTACGGAACAGGCGTAGATGGCGGA
AACATCACCAACTCAAACGTCCCGTACCGTCTGATGAACTCTTGCGGTGGTTTTCTCAGTCGCTATGGCAGCTACAGTAC
CGCACAGATCAGTGCCGCAATGAGTTATATGTTCGGTGCCAATGATGGCAACAGCCCGGATGATATCGCCAATACGAAAC
TGGTCGTTATGTTCGGAAATAACCCGGCAGAAACGCGGATGAGCGGTGGTGGTGTCACTTACTACGTCGAGCAAGCCCGC
GAACGTTCAAACGCACGCATGATCGTCATCGATCCACGTTATAACGACACAGCTGCCGGGCGTGAAGATGAATGGCTGCC
CATTCGCCCTGGCACCGATGGCGCACTGGCCTGTGCGATTGCCTGGGTACTGATTAGTGAAAACATGGTCGATCAGCCAT
TTCTCGACAAATATTGTGTTGGTTACGATGAAAAAACGCTGCCCGCCAACGCACCACGTAACGCGCATTATAAAGCCTAT
ATTCTGGGCGAAGGGCCTGACGGCATAGCTAAAACGCCGGAATGGGCAGCAAAAATCACCAGCATCCCGGCAGAAAAAAT
TATCCAGTTGGCACGAGAGATCGGTTCAGCAAAACCTGCTTATATTTGTCAGGGTTGGGGGCCACAACGACATTCCAACG
GCGAGCAAACATCCCGCGCTATTGCCATGCTTTCCGTTCTCACCGGTAACGTCGGCATAAACGGCGGCAACTCAGGTGTA
CGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTAC
ATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCC
CCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCAATGAGGTGCTT
CAGGATGACAGCAAGTGCGAGATGATTGTTGGCATTGACCACTTCATGACGGCCTCGGCTAAGTATTGCGATATCCTGTT
GCCCGACCTGATGCCAACAGAGCAAGAAGACCTTATCTCTCATGAATCTGCAGGGAATATGGGCTATGTGATCCTCGCCC
AACCCGCAACCTCAGCAAAATTTGAACGCAAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCGGAC
GTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCC
TGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCC
GCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAACGACTGGCG
AAGATTGCAGATACCTGGGAATTGAAAAAAGATGAAATTATTCATCCCCTTCCTGCGTATACCCCAGGTTTTGATGGCTG
GGACGATCCCCTGCGGAAAACCTATCCACTGCAGTTAACGGGCTTCCACTACAAAGCGCGTACCCACTCCAGCTACGGCA
ATATTGATGTGTTACAGCAGGCCTGCCCACAAGAGGTGTGGATCAACCCCATTGATGCTCAGGCTCGCGGTATCCGTCAT
GGCGATACCGTGCGGGTATTTAACAATAATGGAGAAATGCTGATTGCCGCAAAAGTGACTCCGCGTATTCTGCCTGGCGT
TACCGCCATCGGGCAAGGTGCGTGGCTTAAAGCGGATATGTTTGGTGACCGGGTCGATCACGGCGGCAGTATCAATATTC
TGACCTCTCACCGCCCTTCACCGCTGGCAAAGGGAAACCCGTCGCACAGCAATCTTGTCCAGATCGAAAAGGTTTAA

Upstream 100 bases:

>100_bases
CGAAAAAATATAACCCACTACAACCATAAATTCTGGCATGACATTTTGTTTGAAAAGCAATAAGTGAGTAATGATGAAAA
TCCATACCACAGAGGCGCTC

Downstream 100 bases:

>100_bases
GGAGTAGCCCATGACCACACAATATGGATTTTTTATCGATTCCAGCCGCTGTACTGGCTGTAAAACCTGCGAACTGGCGT
GCAAAGATTTTAAAGATCTT

Product: anaerobic dimethyl sulfoxide reductase, A subunit

Products: NA

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDD
VYGNHQVRACLRGRSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHVLYGTGVDGG
NITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDIANTKLVVMFGNNPAETRMSGGGVTYYVEQAR
ERSNARMIVIDPRYNDTAAGREDEWLPIRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAY
ILGEGPDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAMLSVLTGNVGINGGNSGV
REGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVL
QDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRLGPD
VYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLA
KIADTWELKKDEIIHPLPAYTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRH
GDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSHSNLVQIEKV

Sequences:

>Translated_798_residues
MKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDD
VYGNHQVRACLRGRSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHVLYGTGVDGG
NITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDIANTKLVVMFGNNPAETRMSGGGVTYYVEQAR
ERSNARMIVIDPRYNDTAAGREDEWLPIRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAY
ILGEGPDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAMLSVLTGNVGINGGNSGV
REGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVL
QDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRLGPD
VYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLA
KIADTWELKKDEIIHPLPAYTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRH
GDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSHSNLVQIEKV
>Mature_798_residues
MKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDD
VYGNHQVRACLRGRSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHVLYGTGVDGG
NITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDIANTKLVVMFGNNPAETRMSGGGVTYYVEQAR
ERSNARMIVIDPRYNDTAAGREDEWLPIRPGTDGALACAIAWVLISENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAY
ILGEGPDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAMLSVLTGNVGINGGNSGV
REGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVL
QDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRLGPD
VYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLA
KIADTWELKKDEIIHPLPAYTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRH
GDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSHSNLVQIEKV

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI171474008, Length=798, Percent_Identity=99.749373433584, Blast_Score=1668, Evalue=0.0,
Organism=Escherichia coli, GI1787870, Length=801, Percent_Identity=70.6616729088639, Blast_Score=1224, Evalue=0.0,
Organism=Escherichia coli, GI87081797, Length=809, Percent_Identity=65.6365883807169, Blast_Score=1109, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=753, Percent_Identity=30.2788844621514, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1787231, Length=751, Percent_Identity=30.2263648468708, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI87081994, Length=840, Percent_Identity=28.452380952381, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI3868721, Length=781, Percent_Identity=24.583866837388, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI3868720, Length=464, Percent_Identity=24.5689655172414, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI3868719, Length=459, Percent_Identity=22.6579520697168, Blast_Score=83, Evalue=9e-17,
Organism=Escherichia coli, GI1788534, Length=910, Percent_Identity=22.4175824175824, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 88926; Mature: 88926

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAAEAPVEEKAVWSSCTVNCGSRCLL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEE
RLHVKDDTVYWVESDTTGDDVYGNHQVRACLRGRSIRRRMNHPDRLKYPMKRVGKRGEGK
EEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCC
FERISWDEALDTISDNLRRILKDYGNEAVHVLYGTGVDGGNITNSNVPYRLMNSCGGFLS
EEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RYGSYSTAQISAAMSYMFGANDGNSPDDIANTKLVVMFGNNPAETRMSGGGVTYYVEQAR
HHCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEECCCCHHCEECCCCEEEEEHHHH
ERSNARMIVIDPRYNDTAAGREDEWLPIRPGTDGALACAIAWVLISENMVDQPFLDKYCV
HHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHC
GYDEKTLPANAPRNAHYKAYILGEGPDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCC
YICQGWGPQRHSNGEQTSRAIAMLSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVK
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
TQISVFTWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVL
CEEEEEEEECHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHH
QDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAK
CCCCCCEEEEECHHHHHCCCCHHEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCC
FERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGI
CCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC
FKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAY
HHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHCCCCCCCCC
TPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRH
CCCCCCCCCHHHCCCCEEEECEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCHHHCCCCC
GDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPS
CCEEEEECCCCCEEEEEECCCCHHCCCHHCCCCCEEEEHHCCCCCCCCCEEEEEECCCCC
PLAKGNPSHSNLVQIEKV
CCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAAEAPVEEKAVWSSCTVNCGSRCLL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEE
RLHVKDDTVYWVESDTTGDDVYGNHQVRACLRGRSIRRRMNHPDRLKYPMKRVGKRGEGK
EEEEECCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCC
FERISWDEALDTISDNLRRILKDYGNEAVHVLYGTGVDGGNITNSNVPYRLMNSCGGFLS
EEECCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RYGSYSTAQISAAMSYMFGANDGNSPDDIANTKLVVMFGNNPAETRMSGGGVTYYVEQAR
HHCCCCHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEECCCCHHCEECCCCEEEEEHHHH
ERSNARMIVIDPRYNDTAAGREDEWLPIRPGTDGALACAIAWVLISENMVDQPFLDKYCV
HHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHC
GYDEKTLPANAPRNAHYKAYILGEGPDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCC
YICQGWGPQRHSNGEQTSRAIAMLSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVK
EEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
TQISVFTWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTNEVL
CEEEEEEEECHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCEECCCCCCCHHHHHH
QDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAK
CCCCCCEEEEECHHHHHCCCCHHEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCCCCCC
FERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGI
CCCCCHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCC
FKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAY
HHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHCCCCCCCCC
TPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRH
CCCCCCCCCHHHCCCCEEEECEEEEEECCCCCCCHHHHHHHCCHHHCCCCCCHHHCCCCC
GDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPS
CCEEEEECCCCCEEEEEECCCCHHCCCHHCCCCCEEEEHHCCCCCCCCCEEEEEECCCCC
PLAKGNPSHSNLVQIEKV
CCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]