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The map label for this gene is rutB [H]

Identifier: 209400895

GI number: 209400895

Start: 1255269

End: 1255964

Strand: Reverse

Name: rutB [H]

Synonym: ECH74115_1248

Alternate gene names: 209400895

Gene position: 1255964-1255269 (Counterclockwise)

Preceding gene: 209398634

Following gene: 209397669

Centisome position: 22.54

GC content: 55.03

Gene sequence:

>696_bases
ATGATGACGACATTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCA
AAACGCCTATGCCACGCCAGGCGGCTACTTAGATCTCGCCGGGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACA
TTCAAACCGCCGTGACCGCAGCGCGAGCGGCAGGGATGTTGATCATCTGGTTTCAAAATGGCTGGGATGAACAGTATGTC
GAGGCTGGCGGCCCTGGCTCACCGAATTTTCATAAATCGAACGCCCTCAAAACCATGCGTAAACAGCCGCAGCTGCAGGG
GAAATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGGATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGA
AGCCGCGCTACAGCGGTTTCTTCAATACGCCGCTGGACAGCATTTTGCGCAGCCGCGGAATACGCCATCTGGTTTTCACC
AGCATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGATGGCTTTTTTCTGGAGTATTTCGGCGTGGTGCTGGAAGA
CGCAACACACCAGGCGGGGCCGGAATTTGTACAGAAAGCCGCGTTGTTCAATATCGAAACCTTTTTTGGCTGGGTCAGCG
ACGTCGAAACGTTCTGCGACGCGCTTTCTCCCACGTCCTTTGCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
CCTTCGACGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGCGCATTCAACCACTGATGCAGTGCCGCGCCCATCTCCCT
GTGCTGACTCAGGAGGTGGC

Downstream 100 bases:

>100_bases
GGAGTTTAACGATGCCAAAATCCGTAATTATTCCCGCTGGCAGCAGCGCACCGCTGGCCCCCTTCGTTCCCGGCACGCTG
GCTGATGGCGTGGTGTATGT

Product: putative isochorismatase family protein, rutB

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
SIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Sequences:

>Translated_231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
SIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
>Mature_231_residues
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYV
EAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFT
SIATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=98.2608695652174, Blast_Score=469, Evalue=1e-134,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=27.9620853080569, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25418; Mature: 25418

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTA
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
ARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLV
HHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
DELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTSIATNVCVESTLRDGFFLEY
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
FGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure
MMTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTA
CCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
ARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLV
HHHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHH
DELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTSIATNVCVESTLRDGFFLEY
HHHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
FGVVLEDATHQAGPEFVQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA