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The map label for this gene is rutA [H]

Identifier: 209398634

GI number: 209398634

Start: 1255961

End: 1257052

Strand: Reverse

Name: rutA [H]

Synonym: ECH74115_1249

Alternate gene names: 209398634

Gene position: 1257052-1255961 (Counterclockwise)

Preceding gene: 209398214

Following gene: 209400895

Centisome position: 22.56

GC content: 55.49

Gene sequence:

>1092_bases
ATGAAAATTGGCGTATTCGTACCTATTGGCAACAACGGCTGGCTCATTTCGACCCACGCGCCGCAGTACATGCCGACCTT
TGAACTGAATAAAGCCATCGTGCAAAAAGCGGAGCACTACCATTTCGATTTCGCCCTGTCGATGATCAAACTGCGTGGCT
TTGGCGGCAAAACTGAGTTCTGGGATCACAACCTTGAGTCGTTCACCTTGATGGCGGGGCTGGCGGCCGTGACCTCGCGC
ATTCAGATTTACGCCACCGCCGCCACCTTAACGTTACCTCCGGCAATCGTCGCCCGTATGGCCGCAACCATCGACTCCAT
CTCTGGCGGGCGTTTTGGCGTCAACCTCGTGACTGGCTGGCAAAAGCCCGAGTATGAGCAGATGGGTATCTGGCCTGGCG
ATGACTATTTCTCCCGTCGTTACGACTATCTCACCGAGTATGTTCAGGTGCTGCGCGACCTGTGGGGCACGGGAAAAAGC
GATTTTAAAGGCGATTTTTTCACCATGAATGATTGTCGCGTCAGTCCGCAACCGAGTGTCCCCATGAAAGTGATCTGCGC
CGGGCAAAGCGACGCTGGCATGGCGTTCTCCGCTCAGTATGCCGATTTCAACTTCTGTTTCGGCAAAGGCGTAAATACAC
CCACGGCTTTCGCCCCGACCGCTGCGCGGATGAAACAGGCCGCAGAGCAAACCGGGCGCGACGTTGGCTCTTATGTATTG
TTTATGGTGATTGCCGATGAAACCGACGATGCCGCTCGCGCCAAATGGGAACACTACAAAGCGGGCGCGGATGAAGAGGC
GTTAAGCTGGCTAACCGAACAAAGTCAGAAAGATACCCGCTCCGGTACTGACACCAACGTCCGTCAGATGGCCGATCCCA
CTTCGGCGGTAAACATCAATATGGGGACGTTAGTCGGTTCTTACGCCAGTGTCGCGCGCATGTTAGATGAAGTCGCAAGC
GTGCCTGGTGCCGAAGGCGTGCTGTTAACCTTCGACGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGCGCATTCAACC
ACTGATGCAGTGCCGCGCCCATCTCCCTGTGCTGACTCAGGAGGTGGCATGA

Upstream 100 bases:

>100_bases
GGCATCCGCTTTGCAAACAAGCCAATACGCGGCTGAAAAGGATATGCAGAATGCAGCGCCCCGTCTGACTTTCACTTTAC
GAGACGAAGAGAGGTTGATG

Downstream 100 bases:

>100_bases
TGACGACATTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCAAAAC
GCCTATGCCACGCCAGGCGG

Product: putative monooxygenase rutA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSR
IQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFAPTAARMKQAAEQTGRDVGSYVL
FMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVAS
VPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA

Sequences:

>Translated_363_residues
MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSR
IQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFAPTAARMKQAAEQTGRDVGSYVL
FMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVAS
VPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA
>Mature_363_residues
MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSR
IQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFAPTAARMKQAAEQTGRDVGSYVL
FMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVAS
VPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQEVA

Specific function: Catalyzes the pyrimidine ring opening between N-3 and C- 4 by an unusual flavin hydroperoxide-catalyzed mechanism to yield ureidoacrylate peracid. It cleaves pyrmidine rings directly by adding oxygen atoms, making a toxic ureidoacrylate peracid product wh

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family. RutA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787247, Length=363, Percent_Identity=99.4490358126722, Blast_Score=755, Evalue=0.0,
Organism=Escherichia coli, GI1787166, Length=305, Percent_Identity=24.5901639344262, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR019914
- InterPro:   IPR016048 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: NA

Molecular weight: Translated: 39944; Mature: 39944

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEF
CEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
WDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGW
CCCCCHHHHHHHHHHHHHHCEEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECC
QKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSV
CCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCC
PMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFAPTAARMKQAAEQTGRDVGSYVL
CEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHCEEE
FMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNIN
EEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEE
MGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EVA
HCC
>Mature Secondary Structure
MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDFALSMIKLRGFGGKTEF
CEEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
WDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAATIDSISGGRFGVNLVTGW
CCCCCHHHHHHHHHHHHHHCEEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECC
QKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKSDFKGDFFTMNDCRVSPQPSV
CCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCC
PMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFAPTAARMKQAAEQTGRDVGSYVL
CEEEEEECCCCCCCEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHCEEE
FMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNIN
EEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEE
MGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPVLTQ
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EVA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA