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The map label for this gene is yafN [H]

Identifier: 209400393

GI number: 209400393

Start: 292452

End: 292745

Strand: Direct

Name: yafN [H]

Synonym: ECH74115_0277

Alternate gene names: 209400393

Gene position: 292452-292745 (Clockwise)

Preceding gene: 209399967

Following gene: 209398666

Centisome position: 5.25

GC content: 41.16

Gene sequence:

>294_bases
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Upstream 100 bases:

>100_bases
GACGTTGCTTGACCCTCAAATGGAAAGACAACTGGTGCTGGGATTATGATGTATACTATTATGTATATTCTGGTGTGCAT
TATTATGAGGGGATCACTGT

Downstream 100 bases:

>100_bases
GGATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATT
TCCTATAAGCGTGACGGTGT

Product: putative antitoxin of the YafO-YafN toxin-antitoxin system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 97; Mature: 97

Protein sequence:

>97_residues
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Sequences:

>Translated_97_residues
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
>Mature_97_residues
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Specific function: Part of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin; overexpression prevents YafO-mediated cessation of cell growth and inhibition of cell proliferation [H]

COG id: COG2161

COG function: function code D; Antitoxin of toxin-antitoxin stability system

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786426, Length=97, Percent_Identity=98.9690721649485, Blast_Score=196, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006442 [H]

Pfam domain/function: PF02604 PhdYeFM [H]

EC number: NA

Molecular weight: Translated: 11234; Mature: 11234

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKK
CCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCC
PIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE
CHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKK
CCCCCCCCCCCHHHHHCCHHHHHHCCCHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCC
PIKARFRPSAARLEEITRRAEKYLNDMTDDDFNDFKE
CHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]