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The map label for this gene is yafO [H]

Identifier: 209398666

GI number: 209398666

Start: 292748

End: 293146

Strand: Direct

Name: yafO [H]

Synonym: ECH74115_0278

Alternate gene names: 209398666

Gene position: 292748-293146 (Clockwise)

Preceding gene: 209400393

Following gene: 209399255

Centisome position: 5.25

GC content: 43.11

Gene sequence:

>399_bases
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Upstream 100 bases:

>100_bases
TCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCTGAAAAATATCTTAATGATATGACGGATGATGATTTC
AATGACTTTAAGGAATAAGG

Downstream 100 bases:

>100_bases
ATTTATATTATGAATAACATACAAATAAGAAACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGC
GGTTATGATGACCGCCAGTC

Product: putative toxin YafO

Products: NA

Alternate protein names: Endoribonuclease YafO; Toxin YafO [H]

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Sequences:

>Translated_132_residues
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
>Mature_132_residues
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Specific function: Part of a toxin-antitoxin (TA) module. A translation- dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786427, Length=132, Percent_Identity=98.4848484848485, Blast_Score=272, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020353
- ProDom:   PD082093 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 15474; Mature: 15474

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAH
CCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEE
IHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHK
EEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHCCCCHHHH
IGKMAEAFRMRF
HHHHHHHHHCCC
>Mature Secondary Structure
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAH
CCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHEEEEEEE
IHLANVNNPFPPQLRQFSRTNDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHK
EEEECCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHCCCCHHHH
IGKMAEAFRMRF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]