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The map label for this gene is ascB [H]

Identifier: 209400081

GI number: 209400081

Start: 3669154

End: 3670578

Strand: Direct

Name: ascB [H]

Synonym: ECH74115_3967

Alternate gene names: 209400081

Gene position: 3669154-3670578 (Clockwise)

Preceding gene: 209396010

Following gene: 209397638

Centisome position: 65.85

GC content: 52.28

Gene sequence:

>1425_bases
ATGTCAGTATTTCCAGAAGGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGG
CAAAGGACTGACAACGGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTC
AGTTGCGCGATGACGAGTTTTATCCCAGCCATGAGGCGACGGGTTTTTATCATCGTTATAAAGAAGATATCGCCCTGATG
GCAGAGATGGGATTCAAAGTGTTCCGTACCTCAATTGCCTGGAGCCGCCCCTTCCCGCAGGGTGATGAACTGACGCCCAA
CCAGCAGGGCATTGCTTTTTACCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACATTGTGCC
ACTTCGATGTGCCGATGCAGCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGTTTTTCAGCCGCTAC
GCCAGGACCTGCTTTGAAGCATTTGATGGTCTGGTGAAATACTGGCTAACCTTCAATGAAATCAACATTATGTTGCATAG
CCCGTTCTCCGGCGCGGGGCTGGTGTTTGAAGAAGGTGAAAATCAGGATCAGGTGAAATATCAGGCCGCGCATCACCAGC
TGGTTGCCAGTGCGCTAGCCACCAAAATCGCCCATGAGGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGT
AACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGATCGGGAAAACCTGTTTTTTATCGA
TGTGCAGGCGCGGGGCGCGTATCCGGCTTACTCTGCTCGCGTATTCCGCGAAAAAGGGGTAACCATCGACAAAGCACCGG
GCGATGATGAGATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATG
AACACCAACAATAGCAGTGCGGCGAACGTAGTGAAATCGCTGCGTAACCCGTATCTACAGGTGAGCGACTGGGGCTGGGG
AATTGATCCACTCGGTCTGCGTATCACCATGAACATGATGTACGACCGCTATCAGAAGCCGCTGTTTCTGGTGGAAAACG
GCCTCGGCGCAAAAGATGAACTTGCTGCCAATGGCGAGATTAACGATGACTATCGCATCAGCTATTTACGCGAACATATC
CGCGCAATGGGCGAAGCGATTGCAGATGGCATTCCGCTGATGGGCTACACCACATGGGGCTGTATTGATTTAGTTTCCGC
CTCTACGGGTGAAATGAGCAAACGCTACGGTTTTGTCTTTGTTGACCGTGACGACGCAGGCAACGGCACGCTGACGCGCA
CACGTAAGAAATCGTTCTGGTGGTATAAAAAAGTGATTGCCAGTAATGGGGAAGATTTAGAGTAG

Upstream 100 bases:

>100_bases
TTGAGGATATTCCTGTTGAGGAAGCGGCTGCCGAGGCGCGAAAGCATCAGAGCGCACAACCGACCGTCGCCAAAGAAGTA
AGTCTTAATTGAGGATGAAA

Downstream 100 bases:

>100_bases
GTAACCTACTGTCGCACTAACCAGGAAGGCCCGATAAGCGTTGCGCATCGGGCAATCTGGGGTTTGCTATCAGTTCTGAT
TCCCTCATCAAGAACATCCC

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALM
AEMGFKVFRTSIAWSRPFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEM
NTNNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Sequences:

>Translated_474_residues
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALM
AEMGFKVFRTSIAWSRPFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEM
NTNNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
>Mature_473_residues
SVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATGFYHRYKEDIALMA
EMGFKVFRTSIAWSRPFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRYA
RTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGGN
FYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMN
TNNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIR
AMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=29.6593186372746, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI32481206, Length=496, Percent_Identity=29.2338709677419, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI13273313, Length=487, Percent_Identity=27.7207392197125, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI24497614, Length=489, Percent_Identity=26.3803680981595, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28376633, Length=491, Percent_Identity=23.4215885947047, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=97.8902953586498, Blast_Score=974, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=54.2194092827004, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=51.255230125523, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17552856, Length=485, Percent_Identity=30.3092783505155, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17539390, Length=492, Percent_Identity=30.0813008130081, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21356577, Length=503, Percent_Identity=24.6520874751491, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53731; Mature: 53600

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGDELTPNQQGIAFYRSVFEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNTNNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATGFYHRYKEDIALMAEMGFKVFRTSIAWSRPFPQGDELTPNQQGIAFYRSVFEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMQLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNTNNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDELAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVFVDRDDAGNGTLTRTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCEEECHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]