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The map label for this gene is ascF [H]

Identifier: 209396010

GI number: 209396010

Start: 3667688

End: 3669145

Strand: Direct

Name: ascF [H]

Synonym: ECH74115_3966

Alternate gene names: 209396010

Gene position: 3667688-3669145 (Clockwise)

Preceding gene: 209398985

Following gene: 209400081

Centisome position: 65.82

GC content: 56.38

Gene sequence:

>1458_bases
ATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACGCA
CTGTATGACGCGTTTGCGCTTTGTTATCAAAGATGATGCGCTTATCGACAGCCCGACGTTAAAAACCATCCCCGGCGTGC
TCGGCGTGGTACGTAGTGACAACCAGTGTCAGGTGATTATTGGCAATACCGTTTCACAAGCCTTTCAGGAAGTCGTCAGC
CTGCTGCCGGGAGATATGCAGCCCGCCCTGCCCGTGGGTAAACCCAAACTCACGCTGCGTCGCATTGGTGCGGGGATCCT
CGATGCGCTGATCGGCACCATGTCACCGCTGATCCCGGCGATTATCGGCGGATCGATGGTCAAACTGCTGGCGATGATCC
TCGAGATGAGCGGCGTGCTGACAAAAGGATCGCCGACCTTAACCATTCTGAATGTGATTGGTGACGGTGCTTTCTTCTTC
CTGCCGCTGATGGTCGCGGCTTCGGCCGCCATCAAATTTAAAACCAATATGTCGCTGGCGATTGCCATCGCGGGTGTGCT
GGTACATCCGAGTTTTATTGAACTGATGGCGAAAGCGGCCCAGGGTGAACATGTCGAATTTGCCCTGATTCCAGTCACCG
CGGTGAAATACACCTACACAGTGATCCCGGCGCTGGTGATGACCTGGTGCCTGTCATATATCGAACGCTGGGTGGATCGC
ATTACACCGGCGGTGACCAAAAACTTCCTCAAGCCAATGCTGATTGTGTTGATTGCCGCACCGCTGGCAATCCTGCTGAT
TGGCCCGATTGGTATCTGGATCGGTAGCGCCATTTCGGCGCTGGTTTACACCATTCATGGTTATCTGGGCTGGCTTTCAG
TCGCCATTATGGGCGCGCTGTGGCCTCTGCTGGTAATGACCGGGATGCACCGCGTCTTTACGCTAACCATCATTCAGACC
ATTGCCGAAACCGGCAAAGAAGGGATGGTCATGCCGTCAGAGATCGGCGCTAACCTGTCGCTGGGCGGTTCATCACTGGC
GGTGGCGTGGAAAACGAAAAACTCGGAACTGCGCCAGACTGCGCTGGCTGCGGCGGCATCAGCCATTATGGCGGGGATTT
CCGAACCGGCGTTATACGGCGTGGCGATCCGCCTGAAACGTCCGCTTATCGCCAGTCTTATCAGCGGTTTTATTTGCGGC
GCGGTTGCCGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCC
GGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGC
TCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCGAGGCGCGAAAGCATCAGAGCGCACAACCGACCGTCGCCAAA
GAAGTAAGTCTTAATTGA

Upstream 100 bases:

>100_bases
TGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTTCCCTAC
TGATAACAGGATAAAATCCG

Downstream 100 bases:

>100_bases
GGATGAAAATGTCAGTATTTCCAGAAGGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGT
GAAGGTGGCAAAGGACTGAC

Product: PTS system cellobiose/arbutin/salicin-specific transporter subunits IIBC

Products: NA

Alternate protein names: EIIBC-Asc; EII-Asc; Arbutin-, cellobiose-, and salicin-specific phosphotransferase enzyme IIB component; PTS system arbutin-, cellobiose-, and salicin-specific EIIB component; Arbutin, cellobiose, and salicin permease IIC component; PTS system arbutin-, cellobiose-, and salicin-specific EIIC component [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVS
LLPGDMQPALPVGKPKLTLRRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICG
AVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAEARKHQSAQPTVAK
EVSLN

Sequences:

>Translated_485_residues
MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVS
LLPGDMQPALPVGKPKLTLRRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQT
IAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICG
AVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAEARKHQSAQPTVAK
EVSLN
>Mature_484_residues
AKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSL
LPGDMQPALPVGKPKLTLRRIGAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFL
PLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDRI
TPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTLTIIQTI
AETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNSELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGA
VAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAEARKHQSAQPTVAKE
VSLN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI48994906, Length=485, Percent_Identity=98.5567010309278, Blast_Score=948, Evalue=0.0,
Organism=Escherichia coli, GI1790159, Length=457, Percent_Identity=31.2910284463895, Blast_Score=201, Evalue=9e-53,
Organism=Escherichia coli, GI2367362, Length=418, Percent_Identity=27.7511961722488, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788769, Length=488, Percent_Identity=25.4098360655738, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 51034; Mature: 50903

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
NQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFLPLMVAASAAIKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
HHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNSELRQT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCHHHHHH
ALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAEARKHQSAQPTVAK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
EVSLN
CCCCC
>Mature Secondary Structure 
AKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDALIDSPTLKTIPGVLGVVRSD
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
NQCQVIIGNTVSQAFQEVVSLLPGDMQPALPVGKPKLTLRRIGAGILDALIGTMSPLIPA
CCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFFLPLMVAASAAIKFKTNMSLA
HHCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHEEECCCHHH
IAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYTYTVIPALVMTWCLSYIERWVDR
HHHHHHHHCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGSAISALVYTIHGYLGWLSVAIMGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WPLLVMTGMHRVFTLTIIQTIAETGKEGMVMPSEIGANLSLGGSSLAVAWKTKNSELRQT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCEEECCCEEEEEEECCCHHHHHH
ALAAAASAIMAGISEPALYGVAIRLKRPLIASLISGFICGAVAGMAGLASHSMAAPGLFT
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
SVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDIPVEEAAAEARKHQSAQPTVAK
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
EVSLN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]