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The map label for this gene is glnG [H]

Identifier: 209399991

GI number: 209399991

Start: 4952895

End: 4954313

Strand: Reverse

Name: glnG [H]

Synonym: ECH74115_5314

Alternate gene names: 209399991

Gene position: 4954313-4952895 (Counterclockwise)

Preceding gene: 209400767

Following gene: 209397284

Centisome position: 88.91

GC content: 56.66

Gene sequence:

>1419_bases
GTGACGTTTATGCAACGAGGGATAGTCTGGGTAGTCGATGACGATAGTTCCATCCGTTGGGTGCTTGAACGTGCGCTCGC
TGGAGCGGGTTTAACCTGTACAACATTTGAGAACGGCGCGGAGGTACTGGAGGCGCTGGCGAGCAAAACGCCGGATGTGC
TGCTTTCAGATATCCGTATGCCGGGAATGGACGGGCTGGCACTGCTCAAGCAGATTAAACAGCGCCATCCGATGCTTCCG
GTCATCATTATGACCGCACATTCCGATCTGGATGCTGCCGTCAGCGCCTATCAACAAGGGGCGTTTGATTATCTGCCCAA
ACCGTTTGATATCGACGAAGCCGTGGCGCTGGTTGAGCGCGCCATCAGTCATTACCAGGAACAGCAGCAGCCGCGTAATG
TTCAGCTTAACGGCCCAACGACCGATATCATCGGCGAAGCGCCAGCCATGCAGGACGTGTTCCGTATTATCGGTCGGCTT
TCGCGTTCTTCTATTAGCGTGCTGATTAACGGCGAATCCGGCACCGGTAAAGAACTGGTCGCTCATGCCCTGCATCGCCA
CAGTCCGCGAGCCAAAGCGCCATTTATCGCTCTGAATATGGCGGCTATCCCGAAGGATTTGATCGAATCAGAACTGTTTG
GTCACGAGAAAGGCGCGTTTACCGGCGCGAATACCATTCGTCAGGGGCGTTTTGAACAGGCTGATGGCGGTACATTATTT
CTCGATGAAATTGGCGATATGCCGCTGGATGTGCAGACGCGTTTGCTGCGCGTGCTGGCAGACGGTCAGTTTTACCGCGT
TGGCGGCTATGCGCCGGTGAAAGTGGATGTGCGGATTATCGCTGCCACTCACCAGAATCTCGAACAGCGGGTGCAGGAAG
GTAAGTTCCGTGAGGATCTGTTCCACCGCCTGAACGTTATCCGCGTTCATCTGCCGCCGCTGCGTGAGCGTCGGGAAGAT
ATTCCCCGCCTGGCGCGCCATTTTTTACAGGTTGCCGCGCGAGAACTGGGCGTAGAAGCGAAGTTGCTGCATCCGGAAAC
CGAAGCCGCGTTGACGCGTCTGGCGTGGCCAGGCAACGTGCGCCAGCTGGAAAACACCTGCCGCTGGCTAACGGTAATGG
CTGCCGGACAGGAAGTGTTGATTCAGGATTTGCCTGGCGAACTGTTTGAATCAACGGTTGCGGAGAGTACTTCGCAAATG
CAACCGGACAGTTGGGCGACGCTTCTTGCGCAGTGGGCAGACAGAGCGCTACGTTCCGGTCATCAAAATCTGCTTTCCGA
AGCGCAGCCAGAGCTGGAGCGGACGTTACTGACGACCGCGTTGCGACATACGCAGGGGCATAAACAGGAGGCGGCGCGGC
TACTCGGCTGGGGCCGCAACACCCTGACGCGTAAGTTAAAAGAGCTGGGGATGGAGTGA

Upstream 100 bases:

>100_bases
TCGCTCGTAATTTGATTGATCAGCATTCAGGCAAAATTGAATTTACCAGTTGGCCAGGGCATACCGAGTTCTCGGTTTAC
CTGCCTATCAGGAAATAAAG

Downstream 100 bases:

>100_bases
TTCACGGCTTGTGTGTAAAGATTGATTATTGAGCGCAAATTGCTGGTATTTTGCGCTTTACTGTTCCGATAAGTTCAGTA
TGATCTTGCCCGGAAAACGG

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTFMQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLP
VIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRL
SRSSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLF
LDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERRED
IPRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQM
QPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Sequences:

>Translated_472_residues
MTFMQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLP
VIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRL
SRSSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLF
LDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERRED
IPRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQM
QPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
>Mature_471_residues
TFMQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPV
IIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLS
RSSISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLFL
DEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDI
PRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQ
PDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=100, Blast_Score=956, Evalue=0.0,
Organism=Escherichia coli, GI1788550, Length=476, Percent_Identity=42.8571428571429, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1790437, Length=462, Percent_Identity=41.991341991342, Blast_Score=344, Evalue=6e-96,
Organism=Escherichia coli, GI1788905, Length=464, Percent_Identity=38.1465517241379, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI87082117, Length=364, Percent_Identity=42.3076923076923, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789233, Length=329, Percent_Identity=43.4650455927052, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1786524, Length=345, Percent_Identity=43.4782608695652, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1789087, Length=367, Percent_Identity=40.3269754768392, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=40.4833836858006, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87082152, Length=336, Percent_Identity=40.4761904761905, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081872, Length=331, Percent_Identity=39.2749244712991, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87081858, Length=312, Percent_Identity=32.0512820512821, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1789828, Length=239, Percent_Identity=35.5648535564854, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1790860, Length=146, Percent_Identity=32.8767123287671, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=30.0813008130081, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=136, Percent_Identity=30.8823529411765, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=100, Percent_Identity=33, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789402, Length=111, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI145693157, Length=121, Percent_Identity=31.404958677686, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1787375, Length=108, Percent_Identity=31.4814814814815, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52635; Mature: 52504

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFMQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRM
CCCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHCCC
PGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVER
CCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHH
AISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELV
HHHHHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
AHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLF
HHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEE
LDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDL
HHHHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHH
FHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNV
HHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCH
RQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSG
HHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFMQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALASKTPDVLLSDIRM
CCCCCCEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCHHHHHHHCCC
PGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYLPKPFDIDEAVALVER
CCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHH
AISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRSSISVLINGESGTGKELV
HHHHHHHHCCCCCEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
AHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFTGANTIRQGRFEQADGGTLF
HHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCEEE
LDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRIIAATHQNLEQRVQEGKFREDL
HHHHCCCCCHHHHHHHHHHHCCCEEEECCCCEEEEEEEEEEHHHHHHHHHHHHCCHHHHH
FHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELGVEAKLLHPETEAALTRLAWPGNV
HHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCH
RQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAESTSQMQPDSWATLLAQWADRALRSG
HHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HQNLLSEAQPELERTLLTTALRHTQGHKQEAARLLGWGRNTLTRKLKELGME
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]