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The map label for this gene is glnL

Identifier: 209400767

GI number: 209400767

Start: 4954316

End: 4955365

Strand: Reverse

Name: glnL

Synonym: ECH74115_5315

Alternate gene names: 209400767

Gene position: 4955365-4954316 (Counterclockwise)

Preceding gene: 209400069

Following gene: 209399991

Centisome position: 88.93

GC content: 54.57

Gene sequence:

>1050_bases
ATGGCAACAGGCACGCAGCCCGATGCTGGGCAGATCCTCAACTCGCTGATTAACAGTATTTTGTTAATCGATGACAACCT
GGCGATCCATTACGCCAACCCCGCCGCGCAACAACTGCTCGCCCAAAGCTCCCGCAAATTGTTTGGTACGCCGTTACCGG
AACTGTTGAGCTACTTCTCATTAAATATTGAGCTGATGCAAGAAAGTCTGGAGGCGGGGCAAGGTTTTACCGATAACGAA
GTGACGCTGGTCATCGACGGTCGCTCGCATATCCTTTCTGTGACGGCCCAGCGTATGCCGGACGGCATGATCCTGCTGGA
GATGGCGCCGATGGATAACCAGCGCCGCTTAAGCCAGGAACAGCTACAACATGCTCAACAAGTTGCTGCCCGTGATTTAG
TGCGTGGCCTGGCACATGAGATTAAAAATCCGCTTGGCGGTTTACGTGGCGCGGCGCAGCTACTCAGCAAAGCATTACCT
GACCCGTCACTGCTGGAATATACCAAAGTGATTATCGAACAGGCGGACCGGCTGCGAAATCTGGTCGACCGTCTGTTGGG
GCCGCAGCTGCCCGGTACGCGCGTTACCGAAAGTATTCACAAAGTAGCTGAACGCGTGGTAACGCTGGTGTCGATGGAAC
TGCCGGACAACGTGCGGTTGATTCGTGATTACGATCCCAGCCTGCCGGAACTGGCGCACGACCCGGATCAAATTGAACAG
GTCTTGCTGAATATTGTGCGCAATGCGCTACAGGCGCTGGGGCCGGAAGGCGGTGAAATCATTCTGCGTACCCGCACCGC
GTTTCAACTGACCTTACACGGCGAACGCTATCGGCTGGCGGCGCGGATTGATGTGGAAGATAACGGGCCAGGCATTCCGC
CTCATTTGCAGGATACGCTGTTTTACCCGATGGTCAGCGGCCGCGAAGGTGGCACCGGGCTTGGCTTATCCATCGCTCGT
AATTTGATTGATCAGCATTCAGGCAAAATTGAATTTACCAGTTGGCCAGGGCATACCGAGTTCTCGGTTTACCTGCCTAT
CAGGAAATAA

Upstream 100 bases:

>100_bases
ATGGGCTTTTTTCTCCGTCAATTCTCTGATGCTTCGCGCTTTTTATCCGTAAAAAGCTATAATGCACTAAAATGGTGCAA
CCTGTTCAGGAGACTGCTTT

Downstream 100 bases:

>100_bases
AGGTGACGTTTATGCAACGAGGGATAGTCTGGGTAGTCGATGACGATAGTTCCATCCGTTGGGTGCTTGAACGTGCGCTC
GCTGGAGCGGGTTTAACCTG

Product: nitrogen regulation protein NR(II)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNE
VTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNVRLIRDYDPSLPELAHDPDQIEQ
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIAR
NLIDQHSGKIEFTSWPGHTEFSVYLPIRK

Sequences:

>Translated_349_residues
MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNE
VTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNVRLIRDYDPSLPELAHDPDQIEQ
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIAR
NLIDQHSGKIEFTSWPGHTEFSVYLPIRK
>Mature_348_residues
ATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEV
TLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALPD
PSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNVRLIRDYDPSLPELAHDPDQIEQV
LLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARN
LIDQHSGKIEFTSWPGHTEFSVYLPIRK

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain

Homologues:

Organism=Escherichia coli, GI1790300, Length=349, Percent_Identity=100, Blast_Score=701, Evalue=0.0,
Organism=Escherichia coli, GI1788549, Length=351, Percent_Identity=28.2051282051282, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1790436, Length=239, Percent_Identity=30.1255230125523, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI87081816, Length=251, Percent_Identity=30.2788844621514, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=27.5423728813559, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082128, Length=232, Percent_Identity=31.4655172413793, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786600, Length=351, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790567, Length=346, Percent_Identity=26.878612716763, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790346, Length=250, Percent_Identity=26.8, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=28.2945736434109, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NTRB_ECO57 (P0AFB6)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F86074
- PIR:   G91227
- RefSeq:   NP_290494.1
- RefSeq:   NP_312818.1
- ProteinModelPortal:   P0AFB6
- SMR:   P0AFB6
- EnsemblBacteria:   EBESCT00000026390
- EnsemblBacteria:   EBESCT00000060483
- GeneID:   915104
- GeneID:   960290
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5405
- KEGG:   ecs:ECs4791
- GeneTree:   EBGT00050000008663
- HOGENOM:   HBG334875
- OMA:   MELMRES
- ProtClustDB:   PRK11073
- BioCyc:   ECOL83334:ECS4791-MONOMER
- GO:   GO:0016020
- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388
- SMART:   SM00091

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 38557; Mature: 38426

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50109 HIS_KIN; PS50112 PAS

Important sites: BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFS
CCCCCCCCHHHHHHHHHHHEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQE
HHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
QLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALPDPSLLEYTKVIIEQADRLRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNVRLIRDYDPSLPELAHDPDQIEQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCHHHHHH
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTL
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCHHHH
FYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTSWPGHTEFSVYLPIRK
HHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC
>Mature Secondary Structure 
ATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSSRKLFGTPLPELLSYFS
CCCCCCCHHHHHHHHHHHEEEEECCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHH
LNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDGMILLEMAPMDNQRRLSQE
HHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHH
QLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALPDPSLLEYTKVIIEQADRLRN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNVRLIRDYDPSLPELAHDPDQIEQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCHHHHHH
VLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGERYRLAARIDVEDNGPGIPPHLQDTL
HHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCEEEEEEEEEECCCCCCCCCCCHHHH
FYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTSWPGHTEFSVYLPIRK
HHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796