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The map label for this gene is yneJ [H]

Identifier: 209399635

GI number: 209399635

Start: 2053815

End: 2054696

Strand: Direct

Name: yneJ [H]

Synonym: ECH74115_2139

Alternate gene names: 209399635

Gene position: 2053815-2054696 (Clockwise)

Preceding gene: 209399847

Following gene: 209400312

Centisome position: 36.86

GC content: 53.29

Gene sequence:

>882_bases
ATGGATCTGACCCAACTGGAGATGTTCAACGCGGTTGCCGAAGCTGGCAGCATTACGCAGGCTGCAGCAATAGTGCATCG
CGTGCCGTCGAATCTCACTACCCGTCTACGCCAGCTGGAAACAGAACTGGGGGTAGATCTGTTTATTCGCGAGAATCAGC
GTTTACGTCTATCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGC
GTTGTCGCTGGCGATGAGCCGCAAGGTTTATTTTCTCTGGGTTCGCTGGAAAGCACCGCAGCAGTTCGCATTCCGGCCAC
GCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGAAGGTG
TGCTGGAGGGAAAACTTAATGCGGCGTTTATTGATGGACCCATTAACCATACTGCCATTGACGGGATACCGGTATACCGC
GAGGAGCTGATGATCGTCACGCCACAAGGTCATGCGCCAGTAATCCGTGCCAGTCAGGTTAATGGCAGTAACATTTATGC
CTTCCGCGCCAATTGTTCGTATCGTCGCCACTTCGAGAGCTGGTTTCATGCTGACGGTGCCGCTCCGGGAACTATCCATG
AGATGGAGTCTTATCACGGAATGTTGGCCTGTGTGGTCGCAGGAGCAGGTATTGCGCTTATTCCGCGCTCTATGCTGGAA
AGTATGCCGGGGCATCACCAGGTTGAAGCGTGGCCGTTAGCTGAGCAATGGTGTTGGCTAACAACCTGGCTGGTCTGGCG
TCGTGGTGCGAAAACGCGTCCGCTCGAGGCATTTATTCAACTACTGGATGTGCCTGACTCGACAAAACAGGGTTACCAAT
GA

Upstream 100 bases:

>100_bases
CGGGGTATCTCCTTTATGAGTCATGGTATGAAGATACGCAGATTTACTCTTGCTTTAAAATGAATAATATTAAGCCACTT
ATTCACGAATCGAGAATATT

Downstream 100 bases:

>100_bases
GCTATTTTTGATAGTTCTTGCGGCTAATATGCTCTATATAGTGATGTTCCGATGACTTATGACTATATGGGGCAAATATG
GTTACGCCAGTAAGCATCAG

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLEAFIQLLDVPDSTKQGYQ

Sequences:

>Translated_293_residues
MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLEAFIQLLDVPDSTKQGYQ
>Mature_293_residues
MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARS
VVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
SMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLEAFIQLLDVPDSTKQGYQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=293, Percent_Identity=97.2696245733788, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=26.027397260274, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=23.7931034482759, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=23.5915492957746, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082132, Length=287, Percent_Identity=22.9965156794425, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=25.390625, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788481, Length=179, Percent_Identity=30.7262569832402, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=21.5277777777778, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=285, Percent_Identity=24.2105263157895, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788297, Length=260, Percent_Identity=25.3846153846154, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=286, Percent_Identity=21.6783216783217, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790399, Length=289, Percent_Identity=21.7993079584775, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789204, Length=247, Percent_Identity=27.9352226720648, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32403; Mature: 32403

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHHCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVIRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCEEEEEEC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLEAFIQLLDVPDSTKQGYQ
HCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELGVDLFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAVRIPATLAEFNRRYPKIQF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCEEEECCHHHHHHHCCCCEEEE
SLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYREELMIVTPQGHAPVIRASQV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCEECCCCEECCCEEEECCCCCCCEEEEEEC
NGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESYHGMLACVVAGAGIALIPRSMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAEQWCWLTTWLVWRRGAKTRPLEAFIQLLDVPDSTKQGYQ
HCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]