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The map label for this gene is tam [H]

Identifier: 209399847

GI number: 209399847

Start: 2045869

End: 2046627

Strand: Direct

Name: tam [H]

Synonym: ECH74115_2132

Alternate gene names: 209399847

Gene position: 2045869-2046627 (Clockwise)

Preceding gene: 209396806

Following gene: 209399635

Centisome position: 36.72

GC content: 54.68

Gene sequence:

>759_bases
ATGTCTGACTGGAACCCCTCTTTATATCTGCACTTTGCCGCTGAACGATCGCGTCCGGCGGTGGAGCTGCTTGCCAGAGT
GCCGCTGGAGAATGTCGATTATGTTGCCGATCTTGGCTGTGGCCCAGGTAACAGCACCGCCCTGCTGCATCAACGTTGGC
CTGCTGCCAGGATAACGGGCATCGACTCGTCTCCGGCGATGATTGCTGAAGCGCGCAGTGCTTTGCCAGACTGCCAGTTC
GTGGAAGCAGACATCCGCAACTGGCAACCGGAACAGGCACTCGATCTGATTTTTGCTAATGCCTCACTGCAATGGCTGCC
CGACCACTACGAATTGTTTCCTCACTTGGTTTCTTTACTTAATCCGCAGGGCGTGCTGGCAGTACAGATGCCAGATAACT
GGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGTCGTTGGCT
GGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGAT
GCCGTCGCACCAGGCGATTATCGATTGGGTGACCGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAAC
AGCAGCTTTTTCTTACGCGCTACCATCAGATGCTGAAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCA
TTTCCGCGTCTGTTTATTGTTGCCCGCCGTACGGAGTAA

Upstream 100 bases:

>100_bases
CCGGGCCGCGTAAAAAACGTCTGTTCAATGGGTTGATGCCGTGAGGCGAATTTATCAGTTTTATCTACAATTGGGGTAAC
GCGCTGACGGGAGTAAATAA

Downstream 100 bases:

>100_bases
ATATCACGTCAGTTGGTAATGGCGATCGGGAAGAACTTTTGCTGGAATTTCGGCTTCATCGTTCATCTGTAAGAGGTCAA
TTTCAATAGCGTTACAGATG

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGNSTALLHQRWPAARITGIDSSPAMIAEARSALPDCQF
VEADIRNWQPEQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLTRYHQMLKEQYPLQENGQILLA
FPRLFIVARRTE

Sequences:

>Translated_252_residues
MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGNSTALLHQRWPAARITGIDSSPAMIAEARSALPDCQF
VEADIRNWQPEQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLA
GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLTRYHQMLKEQYPLQENGQILLA
FPRLFIVARRTE
>Mature_251_residues
SDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGNSTALLHQRWPAARITGIDSSPAMIAEARSALPDCQFV
EADIRNWQPEQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLAG
VHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLTRYHQMLKEQYPLQENGQILLAF
PRLFIVARRTE

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate [H]

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family [H]

Homologues:

Organism=Escherichia coli, GI1787798, Length=252, Percent_Identity=96.8253968253968, Blast_Score=503, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR023149 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.144 [H]

Molecular weight: Translated: 28947; Mature: 28816

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGNSTALLHQRWPAARITG
CCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEC
IDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANASLQWLPDHYELFPHLVSLL
CCCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHEECCCCEECCHHHHHHHHHHHHH
NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLAGVHAYYDILSEAGCEVDIWR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLTRYHQMLKEQYPLQENGQILLA
HHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
FPRLFIVARRTE
ECCEEEEEECCC
>Mature Secondary Structure 
SDWNPSLYLHFAAERSRPAVELLARVPLENVDYVADLGCGPGNSTALLHQRWPAARITG
CCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCEEEECCCCCCEEEC
IDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANASLQWLPDHYELFPHLVSLL
CCCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHEECCCCEECCHHHHHHHHHHHHH
NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGRESLAGVHAYYDILSEAGCEVDIWR
CCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLTRYHQMLKEQYPLQENGQILLA
HHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
FPRLFIVARRTE
ECCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA