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The map label for this gene is ycjZ [C]

Identifier: 209399565

GI number: 209399565

Start: 330404

End: 331288

Strand: Reverse

Name: ycjZ [C]

Synonym: ECH74115_0326

Alternate gene names: 209399565

Gene position: 331288-330404 (Counterclockwise)

Preceding gene: 209398027

Following gene: 209400952

Centisome position: 5.95

GC content: 53.67

Gene sequence:

>885_bases
ATGGCAAAAAGGGAGAACTACAACGAGCTATACCTGTTTATGCAGGTGGTGCGGGAAGGCAGCTTTACCGCAGCGGCTAA
GCGGCTGGGTCTTGCTCAGTCAGGAGTCAGCCGTTCTGTTCGCGAGCTTGAAGAAAGGCTGGGCGTCCAGCTGCTGGTGC
GCACCACGCGTAAACTGTCGCTGACGCAAGCAGGCGAGCAGCTCTACCAGAAGACGGCATCTGGATTTGAAATGTTAGAT
CTTGGGCTTGCCACGCTGGCGCACTATCGAGAGACGCCCTCCGGCACGGTGCGTATCAATGCCAGCCAGCACGCGATTGA
TAAATGCCTGCTGCCAAAGCTGGCGGTATTTAAACAGCGATATCCTGATATCCAGCTTGAACTGATAAATGAGAGCCGGT
TCGTCGATATCATCGAGGAAAGATTCGATGCAGGTGTCCGCCTGGGGCCTGAAGTCAGCCAGGGCATGGTCGCGGTACGC
ATCACGCCCGATATGGAGATGGCAATTGTGGGGACTCCGGAACATTTTCGTCGCTATGGTTTTCCGCAAACCCCGGCGGA
TTTAAAGGCGCATCCCTGTATCGCCTATCAGTTTGCCGACGGCAGCGTATACCAGTGGGAACTCAATCAGGATGATAAAA
AAATCACTCATCAACCCGAAGGACAATGGGCCTTTTCCGACAGCTATATGGAAGCAGAAGCCGCCCGGCTGGGCCTCGGG
TTGGCCTATGTTCCTGTTGAACTGGTCGCGGATGATTTAGAACACGGTAAGCTTATCAGAGTGTTGCAGCGTTACAGCCT
GCGAATGGAGGGATTGTTTCTCTATTACCCTCATCGCAACGTCTCCCCCGCTCTGCGGATGGTCATTGATACATTGAAAA
TCTGA

Upstream 100 bases:

>100_bases
GAGCAGAATTAGTGCTGATTAGGGGGCGATTAATGCTAAGATTAATACCATATTGTTATAAATTAATGCTGAGATTGCCG
GCATGAAGGGGAATGTTTCA

Downstream 100 bases:

>100_bases
ACGTTAACAACGACCGACTCTTCACTGTGCAATATTGTGTCAGGTAGTCACTCTGCGTTCAATTCCAGGGGCCGCTTTGA
GCGAGAAAGAGCGGACTTAT

Product: LysR substrate binding domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSRSVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLD
LGLATLAHYRETPSGTVRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQGMVAVR
ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHQPEGQWAFSDSYMEAEAARLGLG
LAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYYPHRNVSPALRMVIDTLKI

Sequences:

>Translated_294_residues
MAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSRSVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLD
LGLATLAHYRETPSGTVRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQGMVAVR
ITPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHQPEGQWAFSDSYMEAEAARLGLG
LAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYYPHRNVSPALRMVIDTLKI
>Mature_293_residues
AKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSRSVRELEERLGVQLLVRTTRKLSLTQAGEQLYQKTASGFEMLDL
GLATLAHYRETPSGTVRINASQHAIDKCLLPKLAVFKQRYPDIQLELINESRFVDIIEERFDAGVRLGPEVSQGMVAVRI
TPDMEMAIVGTPEHFRRYGFPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHQPEGQWAFSDSYMEAEAARLGLGL
AYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYYPHRNVSPALRMVIDTLKI

Specific function: May have a role in the regulation of oprD expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=40.2730375426621, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=36.7697594501718, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=32.1678321678322, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI145693193, Length=276, Percent_Identity=31.1594202898551, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=28.6206896551724, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=25.8741258741259, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789440, Length=284, Percent_Identity=24.2957746478873, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786448, Length=281, Percent_Identity=28.1138790035587, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788748, Length=129, Percent_Identity=42.6356589147287, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788706, Length=182, Percent_Identity=29.1208791208791, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788481, Length=291, Percent_Identity=28.5223367697594, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI157672245, Length=166, Percent_Identity=30.1204819277108, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=29.0780141843972, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790011, Length=275, Percent_Identity=23.2727272727273, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082132, Length=289, Percent_Identity=24.2214532871972, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=141, Percent_Identity=29.7872340425532, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=261, Percent_Identity=25.6704980842912, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33381; Mature: 33250

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSRSVRELEERLGVQLLVRTTRKLS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEHHHHHHH
LTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINASQHAIDKCLLPKLAVFKQR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
YPDIQLELINESRFVDIIEERFDAGVRLGPEVSQGMVAVRITPDMEMAIVGTPEHFRRYG
CCCEEEEEECCCHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCEEEEECCHHHHHHCC
FPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHQPEGQWAFSDSYMEAEAARLGLG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCC
LAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYYPHRNVSPALRMVIDTLKI
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKRENYNELYLFMQVVREGSFTAAAKRLGLAQSGVSRSVRELEERLGVQLLVRTTRKLS
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEHHHHHHH
LTQAGEQLYQKTASGFEMLDLGLATLAHYRETPSGTVRINASQHAIDKCLLPKLAVFKQR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHH
YPDIQLELINESRFVDIIEERFDAGVRLGPEVSQGMVAVRITPDMEMAIVGTPEHFRRYG
CCCEEEEEECCCHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCEEEEECCHHHHHHCC
FPQTPADLKAHPCIAYQFADGSVYQWELNQDDKKITHQPEGQWAFSDSYMEAEAARLGLG
CCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCC
LAYVPVELVADDLEHGKLIRVLQRYSLRMEGLFLYYPHRNVSPALRMVIDTLKI
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043; 1427017 [H]