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The map label for this gene is yifK

Identifier: 209398945

GI number: 209398945

Start: 4877389

End: 4878774

Strand: Direct

Name: yifK

Synonym: ECH74115_5229

Alternate gene names: 209398945

Gene position: 4877389-4878774 (Clockwise)

Preceding gene: 209398583

Following gene: 209399680

Centisome position: 87.53

GC content: 53.61

Gene sequence:

>1386_bases
ATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGCACCATTGG
CGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGC
TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCG
CATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTATGTGGATGGCGGTGGGGATCTCTGA
AATCACCGCCATTGGCGTTTATGTCCAGTTCTGGTTCCCGGAGATGGCGCAGTGGATACCCGCATTGATCGCAGTGGCGC
TGGTGGCGTTGGCGAATCTGGCAGCGGTGCGGTTGTACGGCGAAATCGAGTTCTGGTTCGCGATGATCAAAGTCACCACG
ATTATCGTGATGATTGTCATTGGCCTGGGCGTGATTTTCTTTGGCTTTGGCAATGGCGGGCAGTCGATTGGTTTTAGCAA
TCTCACAGAGCATGGCGGTTTCTTTGCGGGTGGCTGGAAAGGGTTCCTGACCGCGCTGTGTATTGTGGTGGCGTCCTACC
AGGGCGTGGAGCTGATTGGCATTACTGCCGGTGAAGCGAAGAATCCGCAGGTGACGCTGCGCAGTGCCGTAGGCAAGGTG
CTGTGGCGGATCCTGATTTTCTACGTTGGCGCGATTTTCGTTATCGTCACCATCTTCCCGTGGAATGAAATAGGCAGCAA
CGGCAGCCCGTTCGTACTGACTTTTGCCAAAATCGGTATTACCGCAGCGGCGGGCATTATCAACTTTGTGGTGCTGACGG
CTGCGCTCTCTGGCTGTAACAGCGGCATGTACAGTTGCGGACGTATGCTCTACGCACTGGCGAAAAACCGTCAGTTACCG
GCGGCGATGGCGAAAGTTTCCCGTCACGGCGTACCGGTTGCGGGTGTGGCAGTATCTATTGCTATTCTGCTAATTGGCTC
ATGCCTGAACTACATCATTCCCAATCCGCAGCGTGTGTTTGTCTACGTCTACAGTGCCAGCGTGCTTCCGGGGATGGTGC
CATGGTTTGTGATATTGATAAGCCAGCTGCGTTTTCGGCGTGCACATAAAGCGGCGATTGCCAGCCATCCGTTCCGCTCA
ATCCTGTTCCCGTGGGCCAACTACGTAACAATGGCATTCCTGATTTGCGTTTTGATCGGCATGTACTTTAATGAAGATAC
GCGTATGTCGCTGTTTGTTGGCATCATCTTTATGCTGGCGGTGACGGCGATTTATAAAGTTTTTGGCCTTAATCGCCACG
GGAAAGCGCATAAACTGGAGGAATAA

Upstream 100 bases:

>100_bases
TTGCCATTTCTCCTGAATTACGAAAACATTCGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATG
CAAACACAACACGAGGATTT

Downstream 100 bases:

>100_bases
GCAGCAAAACGCACAAACCGTAACCAAACGCGCAATTTATTTAAAAAGGGACTAGACAGAGGGGGGGGAAGTCCGTATTA
TCCACCCCCGCAACGGCGCT

Product: putative transport protein YifK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYA
HRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLP
AAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRS
ILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNRHGKAHKLEE

Sequences:

>Translated_461_residues
MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYA
HRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLP
AAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRS
ILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNRHGKAHKLEE
>Mature_460_residues
ADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAH
RYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTTI
IVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKVL
WRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPA
AMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRSI
LFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNRHGKAHKLEE

Specific function: Probable amino-acid or metabolite transport protein

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily

Homologues:

Organism=Escherichia coli, GI48994972, Length=461, Percent_Identity=100, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1786789, Length=429, Percent_Identity=44.0559440559441, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1786302, Length=431, Percent_Identity=40.1392111368909, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI1786602, Length=460, Percent_Identity=44.7826086956522, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1790653, Length=464, Percent_Identity=38.5775862068966, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87081915, Length=392, Percent_Identity=39.2857142857143, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI1788480, Length=435, Percent_Identity=39.0804597701149, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI87081708, Length=471, Percent_Identity=36.7303609341826, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1789017, Length=464, Percent_Identity=32.9741379310345, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI2367353, Length=460, Percent_Identity=25.2173913043478, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324061, Length=438, Percent_Identity=35.1598173515982, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6320772, Length=437, Percent_Identity=33.8672768878719, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324924, Length=414, Percent_Identity=32.3671497584541, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324990, Length=447, Percent_Identity=30.4250559284116, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6322892, Length=437, Percent_Identity=30.8924485125858, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6324059, Length=439, Percent_Identity=33.0296127562642, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322967, Length=415, Percent_Identity=30.3614457831325, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321629, Length=404, Percent_Identity=27.970297029703, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6319824, Length=436, Percent_Identity=28.4403669724771, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6321053, Length=421, Percent_Identity=29.2161520190024, Blast_Score=166, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6319608, Length=422, Percent_Identity=28.9099526066351, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324981, Length=410, Percent_Identity=30.4878048780488, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319543, Length=400, Percent_Identity=29.25, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6324553, Length=397, Percent_Identity=30.9823677581864, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319542, Length=403, Percent_Identity=27.2952853598015, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320717, Length=439, Percent_Identity=24.6013667425968, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6320251, Length=400, Percent_Identity=26.5, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6320364, Length=555, Percent_Identity=23.7837837837838, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24666159, Length=360, Percent_Identity=21.9444444444444, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI221512776, Length=360, Percent_Identity=21.9444444444444, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YIFK_ECOLI (P27837)

Other databases:

- EMBL:   M87049
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   H65183
- RefSeq:   AP_004001.1
- RefSeq:   YP_026258.1
- ProteinModelPortal:   P27837
- STRING:   P27837
- EnsemblBacteria:   EBESCT00000004570
- EnsemblBacteria:   EBESCT00000017226
- GeneID:   945400
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5595
- KEGG:   eco:b3795
- EchoBASE:   EB1428
- EcoGene:   EG11459
- eggNOG:   COG1113
- GeneTree:   EBGT00050000009004
- HOGENOM:   HBG492579
- OMA:   KRAINNI
- ProtClustDB:   PRK10746
- BioCyc:   EcoCyc:YIFK-MONOMER
- Genevestigator:   P27837
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 50400; Mature: 50269

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xec50e40)-; HASH(0x12f54d68)-; HASH(0x12df69d0)-; HASH(0x121f444c)-; HASH(0x1306ea84)-; HASH(0x12fff55c)-; HASH(0x12f6d1a4)-; HASH(0x1268366c)-; HASH(0x11fcfd28)-; HASH(0x131a6100)-; HASH(0x123061d0)-; HASH(0x128a63c0)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIM
CCCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFP
HHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTTIIVMIVIGLGVIFFGFGNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
QSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
CCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
SGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVF
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRSILFPWANYVTMAFLICVLIG
EEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNRHGKAHKLEE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure 
ADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPSVLLAYIIAGLFVFFIM
CCCCHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMAVGISEITAIGVYVQFWFP
HHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTTIIVMIVIGLGVIFFGFGNGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
QSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVELIGITAGEAKNPQVTLRSAVGKV
CCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFAKIGITAAAGIINFVVLTAALSGCN
HHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
SGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVAGVAVSIAILLIGSCLNYIIPNPQRVF
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
VYVYSASVLPGMVPWFVILISQLRFRRAHKAAIASHPFRSILFPWANYVTMAFLICVLIG
EEEEECHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MYFNEDTRMSLFVGIIFMLAVTAIYKVFGLNRHGKAHKLEE
HHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503