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The map label for this gene is pflD [H]

Identifier: 209398325

GI number: 209398325

Start: 5053990

End: 5056287

Strand: Direct

Name: pflD [H]

Synonym: ECH74115_5411

Alternate gene names: 209398325

Gene position: 5053990-5056287 (Clockwise)

Preceding gene: 209400450

Following gene: 209397570

Centisome position: 90.7

GC content: 52.96

Gene sequence:

>2298_bases
ATGACGAATCGTATCTCTCGCCTCAAAACTGCACTGTTTGCCAATACCCGTGAAATCTCGCTGGAGCGTGCGCTGCTTTA
TACCGCCAGCCATCGGCAAACCGAAGGCGAACCGGTGATATTGCGCCGGGCGAAAGCAACAGCGTATATCCTTGAACATG
TTGAAATTTCGATTCGTGATGAAGAACTGATTGCCGGTAACCGCACCGTAAAACCGCGCGCCGGGATTATGTCGCCGGAA
ATGGACCCTTACTGGCTGCTGAAAGAGCTGGGTCAATTCCCGACGCGTCCGCAGGACCGTTTTGCTATCAGCGAAGAAGA
TAAACGTATCTACCGCGAAGAGTTGTTCCCGTACTGGGAAAAACGTTCGATGAAAGATTTCATCAACGGGCAGATGACGG
ATGAAGTAAAAGCCGCGACTAGCACGCAGATTTTCAGCATCAACCAGACGGATAAAGGCCAGGGGCACATTATTATTGAT
TACCCGCGCCTGCTGAATCACGGGCTGGGGGAGCTGGTGGCACAGATGCAGCAACATTGTCAGCAACAGCCGGAGAATCA
CTTTTATCAGGCAGCGCTGTTACTGCTCGAAGCCTCGCAGAAACACATTTTGCGTTACGCCGAACTGGCGGAAACGATGG
CGGCAAGCTGCACTGATGCCCAGCGTCGCGAAGAACTGTTGACTATTGCGGAGATCTCCCGCCATAACGCCGAACATAAA
CCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCT
GTCTTTAGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGGCATCTTTAACCCAGGGCGAAGATCCGGCGTTCCTGA
AAGAACTGCTCGAATCTTTATGGGTGAAATGCAACGACATTGTGCTGTTGCGCTCCACCAGCAGCGCGCGTTATTTCGCA
GGTTTCCCGACTGGCTATACCGCACTGCTCGGCGGGTTAACCGAGAACGGACGTAGCGCGGTGAACGTGCTTTCGTTCCT
CTGCCTCGATGCCTATCAAAGCGTACAATTACCGCAACCGAACCTAGGGGTGCGCACTAACGCCTTGATCGACACGCCGT
TCCTGATGAAAACCGCAGAAACCATTCGCCTCGGCACCGGTATTCCGCAAATCTTTAACGATGAAGTGGTGGTGCCAGCG
TTCCTCAACCGTGGCGTTTCGCTGGAAGATGCGCGCGACTATTCCGTAGTGGGCTGTGTGGAATTATCTATTCCCGGCAG
AACCTACGGCTTGCATGACATCGCGATGTTTAATCTGCTGAAAGTGATGGAAATCTGCCTGCATGAAAATGAAGGCAATG
CTGCGCTGACTTATGAAGGTTTACTGGAGCAGATCCGCGCCAAGATCAGCCACTACATCACCCTGATGGTTGAGGGCAGC
AATATTTGTGATATCGGCCATCGCGACTGGGCACCTGTACCGCTGCTCTCGTCTTTTATCAGCGATTGTCTGGAAAAAGG
TCGCGATATTACCGATGGCGGCGCGCGTTATAACTTCTCCGGCGTACAGGGAATTGGTATCGCCAACCTGAGCGATTCTC
TCCATGCGTTGAAAGGGATGGTTTTTGATCAACAGCGTTTAAGTTTTGACGAATTGCTGTCGGTATTAAAAGCCAACTTT
GCAACGCCAGAAGGCGAAAAAGTCCGCGCTCGCTTAATTAACCGCTTTGAGAAATACGGTAACGATATCGACGAGGTGGG
TAACATTAGCGCCGAACTGTTGCGCCACTACTGCAAAGAAGTGGAAAAATACCAGAACCCGCGCGGCGGTTACTTCACGC
CGGGATCGTATACCGTTTCTGCTCACGTCCCGTTGGGATCGGTGGTTGGCGCGACGCCAGACGGTCGTTTTGCCGGAGAA
CAGTTGGCAGACGGCGGCTTGTCACCCATGCTGGGCCAGGACGCACAAGGGCCAACAGCGGTACTGAAGTCAGTCAGTAA
GCTCGATAACACGCTGCTGTCTAACGGTACGTTGCTGAACGTGAAATTCACTCCGGCGACCCTGGAAGGTGAAGCGGGAT
TACGCAAACTGGCCGACTTCTTACGGGCGTTTACCCAGCTTAAGTTGCAGCATATTCAGTTTAACGTGGTGAACGCCGAC
ACGTTGCGGGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGT
CGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAA

Upstream 100 bases:

>100_bases
CTGATGCCATTATGAGCAAAATTGAAGCTCACCTGGCGCAAACCGCTTAAGTTCTTCTCCCGCTCGCAAGGGCGGGTTCG
CTTTCCCACAGGAGTTCCTC

Downstream 100 bases:

>100_bases
CGTTGTGGAAACGCGCCGCAATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTC
GTACGGTGGTCTTTTTTAAA

Product: putative formate acetyltransferase 2

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 765; Mature: 764

Protein sequence:

>765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELGQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDAQRREELLTIAEISRHNAEHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Sequences:

>Translated_765_residues
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPE
MDPYWLLKELGQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDAQRREELLTIAEISRHNAEHK
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFA
GFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPA
FLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANF
ATPEGEKVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGE
QLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
>Mature_764_residues
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEM
DPYWLLKELGQFPTRPQDRFAISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDY
PRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDAQRREELLTIAEISRHNAEHKP
QTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAG
FPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAF
LNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGSN
ICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGMVFDQQRLSFDELLSVLKANFA
TPEGEKVRARLINRFEKYGNDIDEVGNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQ
LADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADT
LREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=765, Percent_Identity=98.8235294117647, Blast_Score=1571, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=805, Percent_Identity=37.0186335403727, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI48994926, Length=641, Percent_Identity=25.4290171606864, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1787131, Length=543, Percent_Identity=24.6777163904236, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 85740; Mature: 85608

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRD
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFAISEEDKRIYREELFPYWE
CHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDAQRREELLTIAEISRHNAEHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
VFDQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVGNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRAKATAYILEHVEISIRD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEC
EELIAGNRTVKPRAGIMSPEMDPYWLLKELGQFPTRPQDRFAISEEDKRIYREELFPYWE
CHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCHHH
KRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIIDYPRLLNHGLGELVAQMQQHC
HHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCCCCEEEEECHHHHHCCHHHHHHHHHHHH
QQQPENHFYQAALLLLEASQKHILRYAELAETMAASCTDAQRREELLTIAEISRHNAEHK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
PQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYMLPFYQASLTQGEDPAFLKELLESL
HHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLTENGRSAVNVLSFLCLDAYQSVQLPQP
CCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
NLGVRTNALIDTPFLMKTAETIRLGTGIPQIFNDEVVVPAFLNRGVSLEDARDYSVVGCV
CCCCCCCCEECCCHHHHHHHHHEECCCCCHHHCCCEEEHHHHCCCCCCCCCCCCEEEEEE
ELSIPGRTYGLHDIAMFNLLKVMEICLHENEGNAALTYEGLLEQIRAKISHYITLMVEGS
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHEEEEEEECC
NICDIGHRDWAPVPLLSSFISDCLEKGRDITDGGARYNFSGVQGIGIANLSDSLHALKGM
CEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCEECCCHHHHHHHHHH
VFDQQRLSFDELLSVLKANFATPEGEKVRARLINRFEKYGNDIDEVGNISAELLRHYCKE
HHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHH
VEKYQNPRGGYFTPGSYTVSAHVPLGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTA
HHHHCCCCCCEECCCCEEEEECCCCCHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCHHH
VLKSVSKLDNTLLSNGTLLNVKFTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNAD
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECHH
TLREAQQRPQDYAGLVVRVAGYSAFFVELSKEIQDDIIRRTAHQL
HHHHHHHCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]