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The map label for this gene is pflC [H]

Identifier: 209397570

GI number: 209397570

Start: 5056292

End: 5057131

Strand: Direct

Name: pflC [H]

Synonym: ECH74115_5412

Alternate gene names: 209397570

Gene position: 5056292-5057131 (Clockwise)

Preceding gene: 209398325

Following gene: 209396067

Centisome position: 90.74

GC content: 55.24

Gene sequence:

>840_bases
GTGGAAACGCGCCGCAATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTAC
GGTGGTCTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGG
TACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGG
ATTGGTCGCGATATCAGCCTTGACGCGCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGG
CGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGGCTGCGGTTGTGGGGCG
TGTCATGCGCCATTGAAACTGCCGGAGACGCGCCAGCCAGCAAGCTGTTACCGCTGGCGAAATTGTGCGATGAAGTGTTG
TTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCG
TTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGTTTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGC
AGCAGGCGCTGGATGTACTGATCCCGCTGAAAATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCGAACCGAAA
TACCGCCTGCTGGGGAAAACATGGTCGATGAAAGAGGTGCCTGCGCCGTCGTCAGCCGATGTGACAACGATGCGCGAAAT
GGCAGAACGGGCCGGATTTCAGGTTACCGTGGGAGGTTAA

Upstream 100 bases:

>100_bases
CTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGAC
AGCGCATCAGCTGTAACGTT

Downstream 100 bases:

>100_bases
AATGGCATACCTGGTGGCAGTAACCGCCTGCGTAAGCGGCGTGGCGCATACATATATGGCGGCGGAACGGCTGGAAAAGT
TGTGCCTGTTAGAGAAGTGG

Product: pyruvate formate lyase II activase

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
METRRNDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFER
IGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPASKLLPLAKLCDEVL
FDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPK
YRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG

Sequences:

>Translated_279_residues
METRRNDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFER
IGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPASKLLPLAKLCDEVL
FDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPK
YRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG
>Mature_279_residues
METRRNDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFER
IGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPASKLLPLAKLCDEVL
FDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPK
YRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=279, Percent_Identity=98.2078853046595, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=34.4481605351171, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787130, Length=274, Percent_Identity=25.5474452554745, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790839, Length=254, Percent_Identity=23.2283464566929, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 31204; Mature: 31204

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METRRNDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKC
CCCCHHHHHHHHEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHH
LHCAKCLRDADECPSGAFERIGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAE
HHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCEEEEHH
FATRFLQRLRLWGVSCAIETAGDAPASKLLPLAKLCDEVLFDLKIMDATQARDVVKMNLP
HHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECHHCCCCC
YRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
METRRNDVARIFNIQRYSLNDGEGIRTVVFFKGCPHLCPWCANPESISGKIQTVRREAKC
CCCCHHHHHHHHEEEEEECCCCCCEEEEEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHH
LHCAKCLRDADECPSGAFERIGRDISLDALEREVMKDDIFFRTSGGGVTLSGGEVLMQAE
HHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEECCCEEEEHH
FATRFLQRLRLWGVSCAIETAGDAPASKLLPLAKLCDEVLFDLKIMDATQARDVVKMNLP
HHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RVLENLRLLVSEGVNVIPRLPLIPGFTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPK
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECHHCCCCC
YRLLGKTWSMKEVPAPSSADVTTMREMAERAGFQVTVGG
EEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]