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The map label for this gene is ilvI [H]

Identifier: 209398207

GI number: 209398207

Start: 90216

End: 91940

Strand: Direct

Name: ilvI [H]

Synonym: ECH74115_0086

Alternate gene names: 209398207

Gene position: 90216-91940 (Clockwise)

Preceding gene: 209396725

Following gene: 209399406

Centisome position: 1.62

GC content: 52.75

Gene sequence:

>1725_bases
ATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGG
AGGCGCAGTCCTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGGTCATGTTTTAGTTCGTCATGAGCAGGCGG
CAGTGCATATGGCCGATGGTCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACC
AATGCGATTACCGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTT
GATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGC
AAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTGGTCGTTGAT
TTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCGGAGTCGGTCAGTATGCGTTCTTACAATCC
CACTACTACCGGACATAAAGGGCAAATTAAGCGCGCTCTGCAAACGCTGGTGGCGGCAAAAAAACCGGTTGTCTACGTAG
GCGGTGGGGCAATCACGGCGGGCTGCCATCAACAGCTGAAAGAAACGGTGGAGGCGTTAAATCTGCCCGTTGTTTCCTCA
TTGATGGGGCTGGGAGCGTTTCCGGCAACGCATCGTCAGGCACTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAA
TATGACGATGCATAACGCGGATGTGATTTTTGCTGTCGGTGTCCGCTTTGACGATCGCACCACTAACAATCTGGCAAAGT
ACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATTCCGATT
GTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGAT
CCGCGACTGGTGGCAGCAGATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAAC
CGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGGGGCGCTTACGTTACGTCCGATGTCGGGCAGCACCAGATG
TTTGCTGCGCTTTATTATCCATTCGACAAACCCCGTCGTTGGATCAACTCCGGTGGCCTCGGCACTATGGGCTTTGGTTT
ACCTGCGGCACTGGGCGTCAAAATGGCGCTGCCAGAAGAAACCGTGGTTTGCGTCACTGGCGACGGCAGTATTCAGATGA
ACATTCAGGAACTGTCTACAGCGTTGCAATATGAACTGCCGGTGCTGGTGGTTAATCTTAATAACCGCTATTTGGGGATG
GTGAAGCAGTGGCAGGACATGATCTATTCCGGTCGTCATTCACAATCTTATATGCAGTCTTTACCAGACTTCGTCCGTCT
GGCGGAAGCCTATGGTCACGTCGGGATCCAGATCTCTCATCCGCAAGAGCTGGAAAGCAAACTTAGCGAGGCGCTTGAAC
AGGTGCGTAATAATCGCCTGGTATTTGTTGATGTCACCGTCGATGGCAGTGAGCACGTCTACCCGATGCAGATTCGCGGG
GGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGA

Upstream 100 bases:

>100_bases
TTATTACCCGGCGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCC
GTCAAACAGTGAGGCAGGCC

Downstream 100 bases:

>100_bases
TTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGT
GGCTACAACATTGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIGHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAITAGCHQQLKETVEALNLPVVSS
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Sequences:

>Translated_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIGHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAITAGCHQQLKETVEALNLPVVSS
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT
>Mature_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIGHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAITAGCHQQLKETVEALNLPVVSS
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGGAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=27.7385159010601, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI21361361, Length=583, Percent_Identity=26.2435677530017, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=99.3031358885017, Blast_Score=1177, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=39.2226148409894, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=34.6642468239564, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=30.3571428571429, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=24.4604316546763, Blast_Score=168, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=27.0944741532977, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=27.0944741532977, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=575, Percent_Identity=25.3913043478261, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=42.8816466552316, Blast_Score=445, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=478, Percent_Identity=24.8953974895398, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922626, Length=508, Percent_Identity=28.3464566929134, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62844; Mature: 62844

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIGHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHHH
LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPANKLPYVWPESV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHHCCCEECCCCC
SMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAITAGCHQQLKETVEALNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHHCCHHHHH
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHHCCCCCHHHHHHHHHCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRAR
EEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QCLKYDTHSEKIKPQAVIETLWRLTKGGAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPQELESKLSEALEQVRNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIGHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHEEEHHHHHHHHHHH
LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPANKLPYVWPESV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHHCCCEECCCCC
SMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAITAGCHQQLKETVEALNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHHHHHCCHHHHH
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHHCCCCCHHHHHHHHHCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRAR
EEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QCLKYDTHSEKIKPQAVIETLWRLTKGGAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPQELESKLSEALEQVRNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]