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The map label for this gene is leuO [H]

Identifier: 209396725

GI number: 209396725

Start: 88954

End: 89898

Strand: Direct

Name: leuO [H]

Synonym: ECH74115_0084

Alternate gene names: 209396725

Gene position: 88954-89898 (Clockwise)

Preceding gene: 209400291

Following gene: 209398207

Centisome position: 1.6

GC content: 45.82

Gene sequence:

>945_bases
ATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTCAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATT
AACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACCCGTGCTGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCA
GTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCT
CGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAATGAATTGCCTGGTTCAGGTTTTGAACC
CGCGAGCAGTGAACGTGTATTTCATCTTTGTGTTTGCAGCCCGTTAGACAGTATTCTGACCTCGCAGATTTATAATCACA
TTGAGCAGATTGCGCCAAATATACATGTTATGTTCAAGTCTTCATTAAATCAGAACACTGAACATCAGCTGCGTTATCAG
GAAACGGAGTTTGTGATTAGTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTGCCATTATTTAAAGATGAAATGGT
GCTGGTAGCCAGCAAAAATCACCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGG
TTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGC
ATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTT
CGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACTAAATAGCAGAACCTGTTATCTCTCCTGGCATGAAG
CTGCCGGGCGTGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAA

Upstream 100 bases:

>100_bases
CTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAA
GTGTGACAGTGGAGTTAAGT

Downstream 100 bases:

>100_bases
CTGAATGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAGTGTAGAATTTTATTCTGAATGTG
TGGGCTCTCTATTTTAGGAT

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTA
RAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR

Sequences:

>Translated_314_residues
MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTA
RAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR
>Mature_313_residues
PEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTAR
AFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQE
TEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQGM
AMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=314, Percent_Identity=99.3630573248408, Blast_Score=651, Evalue=0.0,
Organism=Escherichia coli, GI87081766, Length=285, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786820, Length=286, Percent_Identity=24.8251748251748, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI157672245, Length=295, Percent_Identity=23.0508474576271, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35714; Mature: 35583

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARL
CCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHH
KVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCS
HEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHRPEFTSVP
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEECCEEEEEHHHCCCCCCCCCC
LFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
CCCCCEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKG
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEHHHCCCCCH
HQWMEEQLVSICKR
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PEVQTDHPETAEFSKPQLRMVDLNLLTVFDAVMQEQNITRAAHVLGMSQPAVSNAVARL
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHH
KVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPGSGFEPASSERVFHLCVCS
HEEECCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECC
PLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQETEFVISYEDFHRPEFTSVP
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHEEECCEEEEEHHHCCCCCCCCCC
LFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDRFASFSQPWYDTVDKQASIAYQG
CCCCCEEEEECCCCCCCCCCCHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
MAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVLPLPLKLNSRTCYLSWHEAAGRDKG
HHHHHHHHHHHHHHEEEECHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEHHHCCCCCH
HQWMEEQLVSICKR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]