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The map label for this gene is pldB [H]

Identifier: 209398086

GI number: 209398086

Start: 4908595

End: 4909617

Strand: Direct

Name: pldB [H]

Synonym: ECH74115_5266

Alternate gene names: 209398086

Gene position: 4908595-4909617 (Clockwise)

Preceding gene: 209396889

Following gene: 209398986

Centisome position: 88.09

GC content: 53.57

Gene sequence:

>1023_bases
ATGTTTCAGCAGCAAAAAGACTGGGAAACAAGAGAAAACGCGTTTGCTGCTTTTACCATGGGACCGCTGACTGATTTCTG
GCGTCAGCGTGATGAAGCAGAGTTTACTGGTGTGGATGACATTCCGGTGCGCTTTGTCCGTTTTCGCGCACAGCACCATG
ACCGGGTGGTAGTCATCTGCCCGGGGCGTATTGAGAGTTACGTAAAATATGCGGAACTGGCCTACGATTTGTTCCATTTG
GGGTTTGATGTCTTAATCATCGACCATCGCGGGCAGGGACGTTCCGGTCGCCTGTTAGCCGATCCGCATCTCGGGCATGT
TAATCGCTTTAATGATTATGTTGATGATCTGGCGGCATTCTGGCAACAGGAGGTCCAGCCCGGTCCGTGGCGTAAACGCT
ATATACTGGCACATTCGATGGGCGGTGCGATCTCCACATTATTTCTGCAACGCCATCCAGGTGTATGTGACGCCATTGCG
CTAACTGCGCCAATGTTTGGGATCGTGATTCGTATGCCGTCATTTATGGCACGGCAGATCCTCAACTGGGCCGAAGCGCA
TCCACGTTTCCGTGATGGCTATGCAATAGGCACCGGGCGCTGGCGCGCGTTGCCGTTTGCTATCAACGTACTGACCCACA
GCAGACAGCGATATCGACGTAACTTACGCTTCTATGCTGATGACCCAACGATTCGCGTCGGTGGGCCGACCTACCATTGG
GTACGCGAAAGTATTCTGGCTGGCGAACAGGTGTTAGCCGGTGCGGGTGATGACGCCACGCCAACGCTTCTCTTGCAGGC
TGAAGAGGAACGCGTGGTGGATAACCGCATTCATGACCGTTTTTGTGAACTCCGCACCGCCGCGGGCCATCCTGTCGAAG
GGGGACGACCGCTGGTAATTAAAGGTGCGTACCATGAGATCCTTTTTGAAAAGGACGCAATGCGCTCAGTTGCGCTCCAC
GCCATCGTTGATTTTTTCAACAGGCATAACTCACCCAGCGGAAACCGCTCTACAGAGGTTTAA

Upstream 100 bases:

>100_bases
CGGTGTGTCTATTTTTGTGCCACAATACGCTACTGTCGCAGCGTTGTGTCAGGCACGCTAAAAAAAACGATTTTACGTGG
TTTAAGAGGCAGATTACCCG

Downstream 100 bases:

>100_bases
ATTTCTTATGTACCAGGTTGTTGCGTCTGATTTAGATGGCACGTTACTTTCTCCCGACCATACATTATCCCCTTACGCCA
AAGAAACCCTGAAGCTGCTC

Product: lysophospholipase L2

Products: NA

Alternate protein names: Lecithinase B [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHL
GFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRIHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALH
AIVDFFNRHNSPSGNRSTEV

Sequences:

>Translated_340_residues
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHL
GFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRIHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALH
AIVDFFNRHNSPSGNRSTEV
>Mature_340_residues
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHL
GFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRIHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALH
AIVDFFNRHNSPSGNRSTEV

Specific function: Unknown

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994980, Length=340, Percent_Identity=99.7058823529412, Blast_Score=701, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 38961; Mature: 38961

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVIC
CCCCCCCCHHHHCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
PGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAF
CCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
WQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIALTAPMFGIVIRMPSFMARQI
HHHHCCCCCCHHEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
HHHHHCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHH
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRIHDRFCELRTAAGHPVEGGRPLVI
HHHHHHCCCHHEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
KGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSGNRSTEV
ECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVRFVRFRAQHHDRVVVIC
CCCCCCCCHHHHCCEEEEECCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
PGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADPHLGHVNRFNDYVDDLAAF
CCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEECCCCCCHHHHHHHHHHHHHH
WQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIALTAPMFGIVIRMPSFMARQI
HHHHCCCCCCHHEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRYRRNLRFYADDPTIRVGGPTYHW
HHHHHCCCCCCCCEEECCCCEEEHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHH
VRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNRIHDRFCELRTAAGHPVEGGRPLVI
HHHHHHCCCHHEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEE
KGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSGNRSTEV
ECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]