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The map label for this gene is rhtC

Identifier: 209396889

GI number: 209396889

Start: 4907188

End: 4907802

Strand: Direct

Name: rhtC

Synonym: ECH74115_5264

Alternate gene names: 209396889

Gene position: 4907188-4907802 (Clockwise)

Preceding gene: 209400089

Following gene: 209398086

Centisome position: 88.07

GC content: 52.36

Gene sequence:

>615_bases
ATGTTATTTCTCACCGTCGCCATGGTGCACATTGTGGCGCTTATGAGTCCCGGTCCCGATTTCTTTTTTGTCTCTCAGAC
CGCTGTCAGTCGTTCCCGTAAAGAAGCGATGATGGGCGTGCTGGGTATTACCTGCGGCGTTATGGTTTGGGCAGGGATTG
CGTTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTTGGCGGTGGCCTGTATCTC
TGCTGGATGGGTTACCAGATGCTGCGCGGCGCGCTGAAAAAAGAGGCGGTTTCTGCACCTGCGCCACAGGTCGAGCTGGC
GAAAAGTGGGCGCAGTTTCCTGAAAGGTTTACTGACCAATCTCGCTAATCCGAAAGCGATTATCTACTTTGGCTCGGTGT
TCTCATTGTTTGTCGGTGATAACGTTGGCACTACCGCGCGCTGGGGCATTTTCGCGTTGATTATTGTCGAAACGCTGGCG
TGGTTTACTGTGGTGGCCAGCCTGTTTGCCCTGCCGCAAATGCGCCGTGGTTATCAACGTCTGGCGAAGTGGATTGATGG
TTTTGCCGGGGCGTTATTTGCCGGATTTGGCATTCATTTGATTATTTCGCGGTAA

Upstream 100 bases:

>100_bases
TGCGCATGTCGATGGCGACGACGAAGAGTAGTCAGCAGCATAAAAAAGTGCCAGGATGAAGACTCCGTAAACGTTTCCCC
CGCGAGTCAAATGTATGTTG

Downstream 100 bases:

>100_bases
TGCCAGACGCGTCTTCAGAGTAAGTCGGATAAGGCGTTTACGCCGCATCCGACATTATTTTTCACGCATGCCTCGCCGAT
GCTAACAGCGCTCCCACCAG

Product: threonine efflux system

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYL
CWMGYQMLRGALKKEAVSAPAPQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLA
WFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR

Sequences:

>Translated_204_residues
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYL
CWMGYQMLRGALKKEAVSAPAPQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLA
WFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR
>Mature_204_residues
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYL
CWMGYQMLRGALKKEAVSAPAPQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLA
WFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR

Specific function: Conducts the efflux of threonine

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family

Homologues:

Organism=Escherichia coli, GI48994978, Length=204, Percent_Identity=100, Blast_Score=409, Evalue=1e-116,
Organism=Escherichia coli, GI1786522, Length=206, Percent_Identity=28.1553398058252, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788099, Length=197, Percent_Identity=27.9187817258883, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RHTC_ECO57 (P0AG39)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A98223
- RefSeq:   NP_290455.1
- RefSeq:   NP_312780.1
- ProteinModelPortal:   P0AG39
- EnsemblBacteria:   EBESCT00000027855
- EnsemblBacteria:   EBESCT00000057450
- GeneID:   915151
- GeneID:   960336
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z5344
- KEGG:   ecs:ECs4753
- GeneTree:   EBGT00050000008903
- HOGENOM:   HBG759042
- OMA:   PAMRRGY
- BioCyc:   ECOL83334:ECS4753-MONOMER
- InterPro:   IPR004778
- InterPro:   IPR001123
- TIGRFAMs:   TIGR00949

Pfam domain/function: PF01810 LysE

EC number: NA

Molecular weight: Translated: 22230; Mature: 22230

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x131349c4)-; HASH(0x12f2bac8)-; HASH(0x127095a4)-; HASH(0x123f5298)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHL
CHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQVELAKSGRSFLKGLLTN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLAWFTVVASLFALPQMRRGYQR
CCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKWIDGFAGALFAGFGIHLIISR
HHHHHHHHHHHHHHHHHHHEEEEH
>Mature Secondary Structure
MLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLGITCGVMVWAGIALLGLHL
CHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQVELAKSGRSFLKGLLTN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETLAWFTVVASLFALPQMRRGYQR
CCCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAKWIDGFAGALFAGFGIHLIISR
HHHHHHHHHHHHHHHHHHHEEEEH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796