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The map label for this gene is yjjW [H]

Identifier: 209397872

GI number: 209397872

Start: 5546147

End: 5547010

Strand: Reverse

Name: yjjW [H]

Synonym: ECH74115_5892

Alternate gene names: 209397872

Gene position: 5547010-5546147 (Counterclockwise)

Preceding gene: 209399734

Following gene: 209398157

Centisome position: 99.55

GC content: 55.9

Gene sequence:

>864_bases
ATGAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTT
TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCG
TGCCACAGTATCCGCATCAGGCGTTGCAGATTGTTGACGGCAAAGTGGTGTGGAACGCTGTGGTCTGCGAGCAATGTGAT
ACCTGCCTGAAGAGGTGTCCGCAACATGCCACGCCAATGGCGCAATCCATGAGCGTGGATGAGGTGCTTAGCCATGTCCG
CAAAGCAGTGCTGTTTATCGAAGGGATAACGGTGAGTGGCGGCGAAGCCACGACCCAACTGCCGTTTGTAGTGGCGCTGT
TTACTGCTATCAAAAACGATCCGCAACTGCGCCACCTCACCTGCCTGGTGGACAGTAACGGTATGTTGAGCGAAACCGGC
TGGGAAAAATTGCTCCCGGTGTGCGACGGCGCAATGCTCGATCTCAAAGCGTGGGGGAGCGAATGTCATCAGCAATTAAC
CGGACGCGATAATCAGCAGATTAAGCGCAGCATCTGTTTGCTGGCAGAGCGCGGTAAGCTGGCGGAACTGCGTTTGCTGG
TAATCCCTGGCCAAGTGGATTATTTGCAACACATCGAAGAACTGGCGGCGTTTATCAAGGGAGTCGGCGATGTTCCGGTA
CGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCGGAAGACGTTGAGCC
GTTGGCTGATGCGTTACGGGAGCGCGGGGTGAGCCGGTTAATATTTCCGGCGCTGTATTTGTGA

Upstream 100 bases:

>100_bases
AAATATCGCGCCGAAGGTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACG
CCAGCCGCGCGTGATAAGCC

Downstream 100 bases:

>100_bases
CATGCCGGATACGACCTACGGTTCGGCACAGACTTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATTGTGCG
CCAACTGCCGGATGCGGCGT

Product: radical SAM domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQYPHQALQIVDGKVVWNAVVCEQCD
TCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAAFIKGVGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALRERGVSRLIFPALYL

Sequences:

>Translated_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQYPHQALQIVDGKVVWNAVVCEQCD
TCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAAFIKGVGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALRERGVSRLIFPALYL
>Mature_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQYPHQALQIVDGKVVWNAVVCEQCD
TCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAAFIKGVGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALRERGVSRLIFPALYL

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=98.2578397212544, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI1787130, Length=280, Percent_Identity=27.1428571428571, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31535; Mature: 31535

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQYPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSG
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAAFIKGVGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALRERGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQYPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSG
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQVDYLQHIEELAAFIKGVGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALRERGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]