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The map label for this gene is yahA [H]

Identifier: 209396993

GI number: 209396993

Start: 387739

End: 388827

Strand: Direct

Name: yahA [H]

Synonym: ECH74115_0378

Alternate gene names: 209396993

Gene position: 387739-388827 (Clockwise)

Preceding gene: 209399058

Following gene: 209400607

Centisome position: 6.96

GC content: 45.82

Gene sequence:

>1089_bases
ATGGCTTCGCATGATTTGAGTGTTTTTTTGGAAGAGTTCGGGGCAACGGTGAATTTGACGTTGCCTGGTATCGTGTCCGA
AAAAGAACGGCTTTTACTCAAGCTGCTAATGGAGGGAATGTCGGTTACAGAAATCTCACAGTACAGAAATCGCAGTGCAA
AGACAATTTCACATCAAAAGAAGCAGCTGTATGAAAAACTAGGCATCCAGAGTGATATTACCTTCTGGCGGGATATCTTT
TTTCAATATCATCCGCAAGTCATTAGTGGCACGGGAAATAAAAATAATTTTTATATCCCTGATAACCGTTGTCACCATAT
CGTCACGCCTGAAGCCATCAGTCTGGCGTTGGAAAACCATGAATTTAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGA
CTGGGGTACTGACGGGCTGTGAAGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATCATCCCGCCGGATCAGTTTATT
CCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGT
AAAACATTTGCTGCCGGATAATTTCCATATTGGTATCAACGTCTCGGCGGGTTGTTTTTTGGCCGCGGGATTTGAAAAAG
AATGCCTGAACCTGGTCAAGAAATTAGGAAACGATAAAATCAAACTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCG
GTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCATAACATTACCTTTGCGCTGGATGACTTTGGTACGGG
TTATGCGACCTATCGTTATCTGCAGGCGTTTCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAATGGCGGGTG
TCGACGAAATATCCGGTCATATTGTGGACAATATTGTCGAACTGGCGCGTAAGCCTGGTCTGAGTATCGTGGCGGAAGGG
GTAGAAACCCAGGAGCAGGCGGATTTAATGATCGGTAAAGGCGTTCACTTTTTGCAGGGCTATTTGTACTCTCCGCCAGT
ACCGGGTAATAAATTTATCTCTGAATGGGTAATGAAAGCAGGTGGTTGA

Upstream 100 bases:

>100_bases
AATTCGGCTCTCTGTGTCATTTTCTTGCAGCCAGCTTTATCCGTCAGAGTGAAGACTCGCAGATTTAGCTGGCGCACAAG
ATTTAATCAAGGTGTGTTCA

Downstream 100 bases:

>100_bases
TGTAAACCGCTATTCACAGCGCATCGGGAGGTTGGCAGCGATTAATTCTCCCGATGCAGTAAATCCTGATAGATCCCCGT
TAATATGCCATTTGGCGCAA

Product: LuxR-family transcriptional regulator/cyclic diguanylate phosphodiesterase (EAL) domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTEISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIF
FQYHPQVISGTGNKNNFYIPDNRCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEG
VETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG

Sequences:

>Translated_362_residues
MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTEISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIF
FQYHPQVISGTGNKNNFYIPDNRCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEG
VETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG
>Mature_361_residues
ASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTEISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFF
QYHPQVISGTGNKNNFYIPDNRCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFIP
LAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIPV
TPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMAGVDEISGHIVDNIVELARKPGLSIVAEGV
ETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKAGG

Specific function: Hydrolyzes c-di-GMP (cyclic bis(3'-5') dimeric GMP) to 5'-pGpG, known as PDE-A activity. PDE-B activity, that is hydrolysis of 5'-pGpG to GMP, proceeds only very slowly [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786507, Length=362, Percent_Identity=93.0939226519337, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI87081845, Length=251, Percent_Identity=44.2231075697211, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788502, Length=296, Percent_Identity=33.445945945946, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081980, Length=247, Percent_Identity=39.6761133603239, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI87081743, Length=245, Percent_Identity=32.6530612244898, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87081921, Length=335, Percent_Identity=29.2537313432836, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI226510982, Length=243, Percent_Identity=33.7448559670782, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790496, Length=255, Percent_Identity=30.5882352941176, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787541, Length=239, Percent_Identity=31.7991631799163, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788849, Length=233, Percent_Identity=30.4721030042918, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI87082096, Length=210, Percent_Identity=32.3809523809524, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787055, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001633
- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00563 EAL; PF00196 GerE [H]

EC number: NA

Molecular weight: Translated: 40574; Mature: 40443

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTEISQYRNRSAKTISHQK
CCCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDNRCHHIVTPEAISLALENH
HHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCCCEECCCCCCCEEECHHHHHEECCCC
EFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFIPLAESSGLIVIMTRQLMKQT
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
ADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
HHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCCCCC
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMAGVDEISGH
CCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
IVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKA
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHC
GG
CC
>Mature Secondary Structure 
ASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTEISQYRNRSAKTISHQK
CCCHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHH
KQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDNRCHHIVTPEAISLALENH
HHHHHHHCCCCCHHHHHHHHHHHCCEEEECCCCCCCEECCCCCCCEEECHHHHHEECCCC
EFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFIPLAESSGLIVIMTRQLMKQT
CCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
ADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVKKLGNDKIKLVLELTERNPIP
HHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHCCCEEEEEEEEECCCCCC
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMAGVDEISGH
CCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHH
IVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKA
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 1956285 [H]