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The map label for this gene is phnM [H]

Identifier: 209396880

GI number: 209396880

Start: 5246137

End: 5247273

Strand: Reverse

Name: phnM [H]

Synonym: ECH74115_5608

Alternate gene names: 209396880

Gene position: 5247273-5246137 (Counterclockwise)

Preceding gene: 209396040

Following gene: 209397188

Centisome position: 94.17

GC content: 61.57

Gene sequence:

>1137_bases
ATGATTATCAATAACGTCAAACTGGTGCTGGAAAACGAAGTGGTAAACGGTTCGCTGGAGATGCAGGACGGCGAAATCCG
CGCCTTTGCCGAAAGCCAGAGCCGCCTGCCGGAGGCGATGGACGGCGAAGGCGGCTGGCTGCTGCCGGGGCTGATTGAGC
TGCATACAGACAACCTCGATAAATTCTTCACCCCGCGCCCGAAAGTTGACTGGCCCGCCCACTCGGCGATGAGCAGCCAT
GACGCGCTGATGGTGGCGAGCGGCATCACCACCGTACTGGACGCCGTGGCGATTGGCGACGTGCGAGACGGCGGCGATCG
GCTGGAGAATCTGGAGAAGATGATCAACGCCATCGAAGAGACGCAGAAACGCGGCGTCAACCGCGCCGAGCACCGCCTGC
ACCTGCGCTGCGAGCTGCCGCATAACACCACGCTGCCGCTGTTTGAAAAGCTGGTGCAGCGCGAGCCGGTGACGCTGGTG
TCGCTGATGGATCACTCGCCGGGCCAGCGCCAGTTCGCCAACCGCGAGAAGTATCGCGAATATTATCAGGGCAAATACTC
GCTCACCGATGCGCAGATGCAGCAGTATGAAGAGGAACAGCTGGCGCTTGCCGCACGCTGGTCGCAGCCGAATCGCGAAT
CCATCGCCGCAATGTGCCGCGCGCGACAAATTGCGCTCGCCAGCCACGATGACGCAACGCACGCCCACGTTGCCGAATCT
CACCAGCTTGGCAGCGTGATCGCCGAATTTCCCACCACGTTTGAAGCAGCGGAAGCCTCGCGCAAGCATGGCATGAACGT
GCTGATGGGCGCGCCAAATATCGTGCGTGGCGGTTCGCACTCCGGCAACGTGGCGGCCAGTGAACTGGCACAGCTTGGCC
TGCTGGATATCCTCTCTTCCGACTACTACCCCGCCAGCCTGCTGGATGCGGCGTTCCGCGTCGCCGATGACCAGAGCAAC
CGCTTTACGCTGCCGCAGGCGGTGAAGCTGGTGACCAAAAATCCGGCGCAGGCGCTTAATCTCCAGGATCGCGGGGTGAT
TGGCGAGGGCAAACGCGCCGACCTGGTGCTGGCGCATCGCAAGGGCAATCACATTCATATCGACCACGTCTGGCGTCAGG
GTAAAAGGGTGTTCTGA

Upstream 100 bases:

>100_bases
GAGAAGCCAAAGCCCGCGGCGCAGCCATCGTCGGCATTTTCCATGACGAAGCCGTACGTAATGACGTCGCCGACCGCCTG
CACCCAATGGGAGCCTCTTC

Downstream 100 bases:

>100_bases
TGATGGGAAAACTGATTTGGTTAATGGGGCCGTCCGGCTCCGGGAAAGATAGCCTGCTGGCGGAACTCCGCCTGCGGGAA
CAAACTCAGCTACTGGTGGC

Product: phosphonate metabolism protein PhnM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAMCRARQIALASHDDATHAHVAES
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSN
RFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF

Sequences:

>Translated_378_residues
MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAMCRARQIALASHDDATHAHVAES
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSN
RFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF
>Mature_378_residues
MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSH
DALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLV
SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAMCRARQIALASHDDATHAHVAES
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSN
RFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF

Specific function: Belongs to an operon involved in alkylphosphonate uptake and C-P lyase. Exact function not known [H]

COG id: COG3454

COG function: function code P; Metal-dependent hydrolase involved in phosphonate metabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790533, Length=378, Percent_Identity=98.1481481481482, Blast_Score=769, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059
- InterPro:   IPR012696 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: NA

Molecular weight: Translated: 42008; Mature: 42008

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLD
CEECCEEEEEECCEECCEEEECCCCCCEEHHHHHCCCHHCCCCCCEECCCCEEEECCCHH
KFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEE
HHCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLVSLMDHSPGQRQFANREKYRE
HHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
YYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAMCRARQIALASHDDATHAHVAES
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHH
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSS
HHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHR
CCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEEEC
KGNHIHIDHVWRQGKRVF
CCCEEEHHHHHCCCCCCC
>Mature Secondary Structure
MIINNVKLVLENEVVNGSLEMQDGEIRAFAESQSRLPEAMDGEGGWLLPGLIELHTDNLD
CEECCEEEEEECCEECCEEEECCCCCCEEHHHHHCCCHHCCCCCCEECCCCEEEECCCHH
KFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVRDGGDRLENLEKMINAIEE
HHCCCCCCCCCCCHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TQKRGVNRAEHRLHLRCELPHNTTLPLFEKLVQREPVTLVSLMDHSPGQRQFANREKYRE
HHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHH
YYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAMCRARQIALASHDDATHAHVAES
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHH
HQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVRGGSHSGNVAASELAQLGLLDILSS
HHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
DYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKNPAQALNLQDRGVIGEGKRADLVLAHR
CCCCHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCEEEEEC
KGNHIHIDHVWRQGKRVF
CCCEEEHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]