BacMap

Definition	Escherichia coli O157:H7 str. EC4115, complete genome.
Accession	NC_011353
Length	5,572,075

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The map label for this gene is phnL [H]

Identifier: 209396040

GI number: 209396040

Start: 5247270

End: 5247950

Strand: Reverse

Name: phnL [H]

Synonym: ECH74115_5609

Alternate gene names: 209396040

Gene position: 5247950-5247270 (Counterclockwise)

Preceding gene: 209400505

Following gene: 209396880

Centisome position: 94.18

GC content: 61.09

Gene sequence:

>681_bases
ATGATTAACGTACAAAACGTCAGTAAAACCTTCATCCTGCACCAGCAAAACGGCGTGCGCTTGCCCGTCCTCAACCGCGC
CTCGCTCACCGTCAACGCGGGCGAATGCGTGGTGCTCCACGGCCACTCCGGCAGCGGCAAATCAACCCTGCTGCGCTCGC
TGTACGCCAACTATCTGCCCGATGAAGGGCAAATCCAGATCAAACACGGTGACGAGTGGGTAGACCTGGTCACTGCGCCA
GCGCGCAAAGTGGTGGAAATCCGCAAAACCACCGTTGGCTGGGTGAGCCAGTTTCTGCGCGTTATCCCGCGCATTTCGGC
GCTGGAAGTAGTAATGCAGCCGCTGCTCGATACCGGCGTACCGCGTGAAGCCTGCGCCGCTAAAGCCGCGCGTCTGCTCA
CCCGTCTGAACGTGCCGGAACGCCTGTGGCGCCTTGCGCCATCGACCTTCTCCGGCGGCGAACAGCAGCGCGTCAACATC
GCCCGTGGCTTTATCGTCGACTACCCCATTCTGCTGCTCGACGAACCTACCGCCTCGCTGGACGCCAAAAACAGCGCCGC
CGTAGTCGAACTGATCCGAGAAGCCAAAGCCCGCGGCGCAGCCATCGTCGGCATTTTCCATGACGAAGCCGTACGTAATG
ACGTCGCCGACCGCCTGCACCCAATGGGAGCCTCTTCATGA

Upstream 100 bases:

>100_bases
GATGCGGCGTAAACGCCTTATCCGGCCTACAAATGCGCTCCCCGTAGGTCGGATAAGACGCATCAGCGTCGCATCCGACA
CCCGAACCACGAGGCGAAAA

Downstream 100 bases:

>100_bases
TTATCAATAACGTCAAACTGGTGCTGGAAAACGAAGTGGTAAACGGTTCGCTGGAGATGCAGGACGGCGAAATCCGCGCC
TTTGCCGAAAGCCAGAGCCG

Product: phosphonate C-P lyase system protein PhnL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAP
ARKVVEIRKTTVGWVSQFLRVIPRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADRLHPMGASS

Sequences:

>Translated_226_residues
MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAP
ARKVVEIRKTTVGWVSQFLRVIPRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADRLHPMGASS
>Mature_226_residues
MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAP
ARKVVEIRKTTVGWVSQFLRVIPRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNI
ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADRLHPMGASS

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates. Probably responsible for energy coupling to the transport system [H]

COG id: COG4778

COG function: function code P; ABC-type phosphonate transport system, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI116734710, Length=223, Percent_Identity=26.457399103139, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1790534, Length=226, Percent_Identity=99.1150442477876, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1788225, Length=220, Percent_Identity=34.5454545454545, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787029, Length=221, Percent_Identity=37.10407239819, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI87081834, Length=209, Percent_Identity=33.4928229665072, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787089, Length=193, Percent_Identity=34.1968911917098, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787105, Length=192, Percent_Identity=31.25, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1789672, Length=222, Percent_Identity=31.0810810810811, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1786703, Length=194, Percent_Identity=35.5670103092784, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786398, Length=219, Percent_Identity=30.1369863013699, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789962, Length=201, Percent_Identity=28.3582089552239, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789032, Length=221, Percent_Identity=32.1266968325792, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790544, Length=225, Percent_Identity=32.4444444444444, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1789891, Length=188, Percent_Identity=32.4468085106383, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789873, Length=208, Percent_Identity=28.3653846153846, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788644, Length=204, Percent_Identity=30.3921568627451, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994997, Length=196, Percent_Identity=32.6530612244898, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788761, Length=225, Percent_Identity=32.4444444444444, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1787759, Length=216, Percent_Identity=31.0185185185185, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081709, Length=218, Percent_Identity=31.1926605504587, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1786872, Length=224, Percent_Identity=31.25, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788506, Length=237, Percent_Identity=30.8016877637131, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48995001, Length=226, Percent_Identity=27.4336283185841, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081791, Length=220, Percent_Identity=29.0909090909091, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789991, Length=217, Percent_Identity=27.6497695852535, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693107, Length=235, Percent_Identity=29.7872340425532, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788450, Length=216, Percent_Identity=32.8703703703704, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787500, Length=204, Percent_Identity=25.9803921568627, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1790467, Length=193, Percent_Identity=30.5699481865285, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI48994883, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790190, Length=213, Percent_Identity=28.6384976525822, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787792, Length=211, Percent_Identity=30.3317535545024, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787712, Length=193, Percent_Identity=30.0518134715026, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787370, Length=198, Percent_Identity=28.7878787878788, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1787112, Length=175, Percent_Identity=32.5714285714286, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082267, Length=187, Percent_Identity=29.9465240641711, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1787758, Length=202, Percent_Identity=28.7128712871287, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1786253, Length=157, Percent_Identity=35.6687898089172, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788897, Length=194, Percent_Identity=30.9278350515464, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789864, Length=213, Percent_Identity=27.2300469483568, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1788472, Length=190, Percent_Identity=28.4210526315789, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1790525, Length=217, Percent_Identity=26.7281105990783, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17558664, Length=212, Percent_Identity=33.0188679245283, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71992285, Length=195, Percent_Identity=28.2051282051282, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=221, Percent_Identity=28.5067873303167, Blast_Score=65, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322640, Length=197, Percent_Identity=34.010152284264, Blast_Score=65, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319856, Length=200, Percent_Identity=29, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17136662, Length=186, Percent_Identity=33.8709677419355, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR012701 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 24695; Mature: 24695

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLP
CCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCC
DEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVIPRISALEVVMQPLLDTGV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNIARGFIVDYPILLLDEPTASL
CHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCC
DAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADRLHPMGASS
CCCCHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSGSGKSTLLRSLYANYLP
CCCCCCCCEEEEEECCCCCEEEEECCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCC
DEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVIPRISALEVVMQPLLDTGV
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
PREACAAKAARLLTRLNVPERLWRLAPSTFSGGEQQRVNIARGFIVDYPILLLDEPTASL
CHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCC
DAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADRLHPMGASS
CCCCHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]