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The map label for this gene is frlB

Identifier: 209396714

GI number: 209396714

Start: 4324263

End: 4325285

Strand: Direct

Name: frlB

Synonym: ECH74115_4683

Alternate gene names: 209396714

Gene position: 4324263-4325285 (Clockwise)

Preceding gene: 209396621

Following gene: 209400169

Centisome position: 77.61

GC content: 54.45

Gene sequence:

>1023_bases
ATGTTGGATATTGATAAAAGCACTGTGGACTTTCTGGTCACCGAAAATATGGTTCAGGAAGTGGAAAAAGTTCTCAGCCA
TGACGTTCCGCTGGTGCACACCATCGTGGAAGAGATGGTGAAGCGCGACATCGATCGTATTTATTTCGTTGCCTGCGGAT
CGCCACTCAACGCGGCGCAAACGGCGAAACATCTGGCAGATCGCTTTTCCGATCTTCAGGTTTACGCCATTTCCGGCTGG
GAGTTCTGCGATAACACCCCATATCGCCTCGACGCTCGTTGCGCGGTGATTGGCGTTTCTGACTACGGTAAAACCGAAGA
GGTAATCAAAGCGCTGGAGCTGGGCCGGGCCTGCGGCGCACTCACTGCGGCGTTCACCAAACGCGCGGATAGCCCGATTA
CCTCGGCGGCGGAATTTAGCATTGATTATCAGGCCGACTGTATCTGGGAAATTCATCTGCTGCTCTGCTACAGCGTGGTG
CTGGAGATGATCACCCGCCTCGCGCCGCACGCGGAAATCGGCAAGATCAAAAACGATCTCAAACAGCTGCCGAATGCGCT
CGGTTATCTGGTACGCACTTGGGAAGAAAAAGGCCGCCAGCTTGGTGAACTGGCCAGCCAGTGGCCGATGATTTACACGG
TTGCTGCAGGCCCGCTGCGCCCGCTGGGTTACAAAGAAGGCATCGTGACGCTGATGGAATTTACCTGGACGCACGGCTGT
GTGATTGAGAGCGGTGAGTTCCGTCACGGCCCGCTGGAGATTGTCGAACCGGGCGTTCCGTTCCTGTTCCTGCTCGGCAA
TGATGAAAGTCGCCACACCACCGAACGCGCCATTAACTTTGTTAAACAGCGTACTGACAACGTGATCGTCATCGATTACG
CCGCAATTTCGCAAGGACTGCACCCGTGGCTGGCACCGTTCCTGATGTTCGTGCCAATGGAGTGGCTCTGCTACTACCTG
TCTATTTACAAAGATCACAACCCGGATGAACGCCGCTATTACGGTGGTCTGGTGGAATATTAA

Upstream 100 bases:

>100_bases
CCGTCATCGTTATTGCCACTGGTCTGCCTGCTTACGCCTTCTGGGCGAAGCGTAGCCGCCAGCTCAACGCTTTGTCGTAA
TGTTATCTGGAGAAAATAAA

Downstream 100 bases:

>100_bases
TCCTCTCTCCCGGCCCGGCAACGGGCCGGATTTACGCAAGGAGTTACCCGATGAAAACAGGTATGTTTACCTGCGGCCAC
CAGCGGCTGCCTATTGAACA

Product: fructoselysine-6-P-deglycase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDIDRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGW
EFCDNTPYRLDARCAVIGVSDYGKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGC
VIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTERAINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYL
SIYKDHNPDERRYYGGLVEY

Sequences:

>Translated_340_residues
MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDIDRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGW
EFCDNTPYRLDARCAVIGVSDYGKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGC
VIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTERAINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYL
SIYKDHNPDERRYYGGLVEY
>Mature_340_residues
MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDIDRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGW
EFCDNTPYRLDARCAVIGVSDYGKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
LEMITRLAPHAEIGKIKNDLKQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGC
VIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTERAINFVKQRTDNVIVIDYAAISQGLHPWLAPFLMFVPMEWLCYYL
SIYKDHNPDERRYYGGLVEY

Specific function: Catalyzes the conversion of fructoselysine 6-phosphate to glucose 6-phosphate and lysine

COG id: COG2222

COG function: function code M; Predicted phosphosugar isomerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 SIS domains

Homologues:

Organism=Escherichia coli, GI87082251, Length=340, Percent_Identity=98.5294117647059, Blast_Score=697, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FRLB_ECO57 (Q8X844)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C86002
- PIR:   F91156
- RefSeq:   NP_289919.1
- RefSeq:   NP_312249.1
- ProteinModelPortal:   Q8X844
- SMR:   Q8X844
- EnsemblBacteria:   EBESCT00000026525
- EnsemblBacteria:   EBESCT00000057147
- GeneID:   915923
- GeneID:   958541
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4732
- KEGG:   ecs:ECs4222
- GeneTree:   EBGT00050000009700
- HOGENOM:   HBG467353
- OMA:   LMEMQWI
- ProtClustDB:   PRK11382
- BioCyc:   ECOL83334:ECS4222-MONOMER
- InterPro:   IPR001347

Pfam domain/function: PF01380 SIS

EC number: NA

Molecular weight: Translated: 38547; Mature: 38547

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS51464 SIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDIDRIYFVACGSPLNAAQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCHHH
TAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDYGKTEEVIKALELGRACGA
HHHHHHHHHCCEEEEEEECCHHCCCCCCEEECEEEEEECCCCCCHHHHHHHHHHHHHHHH
LTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVVLEMITRLAPHAEIGKIKNDL
HHHHHHHHCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCE
VIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTERAINFVKQRTDNVIVIDYAAISQGL
EEECCCCCCCCHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCC
HPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYYGGLVEY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDIDRIYFVACGSPLNAAQ
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCHHH
TAKHLADRFSDLQVYAISGWEFCDNTPYRLDARCAVIGVSDYGKTEEVIKALELGRACGA
HHHHHHHHHCCEEEEEEECCHHCCCCCCEEECEEEEEECCCCCCHHHHHHHHHHHHHHHH
LTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVVLEMITRLAPHAEIGKIKNDL
HHHHHHHHCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KQLPNALGYLVRTWEEKGRQLGELASQWPMIYTVAAGPLRPLGYKEGIVTLMEFTWTHGC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCE
VIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTERAINFVKQRTDNVIVIDYAAISQGL
EEECCCCCCCCHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHCCC
HPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYYGGLVEY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796