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The map label for this gene is rlmF [H]

Identifier: 209396578

GI number: 209396578

Start: 970146

End: 971072

Strand: Direct

Name: rlmF [H]

Synonym: ECH74115_0957

Alternate gene names: 209396578

Gene position: 970146-971072 (Clockwise)

Preceding gene: 209397893

Following gene: 209400925

Centisome position: 17.41

GC content: 54.26

Gene sequence:

>927_bases
ATGTCCGCCCAGAAACCGGGGTTGCATCCGCGCAACCGTCATCACAGCCGCTACGATCTCGCCACGCTTTGTCAGGTCAA
TCCTGAACTCAAGCAATTCCTCACGCTTACACCCGCCGGGGAGCAAAGCGTAGACTTTGCCAATCCGCTGGCGGTGAAGG
CGCTCAATAAGGCGTTGCTGGCCCATTTTTACGCCGTAGCGAACTGGGATATCCCGGACGGTTTTCTCTGCCCGCCAGTA
CCGGGCCGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCTGAAGCAAGCGGAACAATTCCAGCTAATGCCAGCAT
TCTTGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTACCGGTAGTG
AAACCAGCAGCCAGGCGTTAAGCAGCGCGCAGGCGATCATCAGTGCTAATCCGGGGCTTAATCGCGCCATTCGTCTGCGT
CGGCAAAAAGAGAGTGGGGCGATTTTTAACGGCATCATCCATAAAAACGAGCAATACGACGCGACCTTGTGTAACCCGCC
ATTCCACGATTCCGCCGCTGCGGCGCGGGCAGGTAGTGAGTGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCAC
TGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAGGTCACCTTTATCAAAAAGATGATTGAAGAGAGC
AAAGGCTTCGCGAAGCAGGTGATGTGGTTTACTTCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCT
GACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCT
TTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAA

Upstream 100 bases:

>100_bases
CCATAGCCATGTTAAGGTTTTGGGGAATGATAAACGCAGCCAGATATTTACCCTGTCGCGTTATGCGGGTATCTTACGCC
GCTGCTTAAAGGAGAATGCT

Downstream 100 bases:

>100_bases
AGCGTTGGCTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACCGGCGC
GCGAACGCCCGCCAGGTCGA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPV
PGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEES
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR

Sequences:

>Translated_308_residues
MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPV
PGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEES
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR
>Mature_307_residues
SAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSVDFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVP
GRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLRR
QKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEESK
GFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=293, Percent_Identity=31.0580204778157, Blast_Score=148, Evalue=7e-36,
Organism=Escherichia coli, GI87081786, Length=308, Percent_Identity=99.025974025974, Blast_Score=638, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564931, Length=306, Percent_Identity=25.4901960784314, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281363415, Length=291, Percent_Identity=29.2096219931272, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922302, Length=291, Percent_Identity=29.2096219931272, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 34129; Mature: 33998

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSVDFANPLAVKALNKALL
CCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHH
AHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHH
IGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLRRQKESGAIFNGIIHKNEQYD
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEHHHHCCCCCCC
ATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEES
CEECCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHEEECCCCHHHHHHHHHHH
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEECHHHH
RRFVNRQR
HHHHCCCC
>Mature Secondary Structure 
SAQKPGLHPRNRHHSRYDLATLCQVNPELKQFLTLTPAGEQSVDFANPLAVKALNKALL
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHEECCCCCCCCCCCCCHHHHHHHHHHHH
AHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIPANASILDIGVGANCIYPL
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHH
IGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLRRQKESGAIFNGIIHKNEQYD
HHHHHCCCEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEHHHHCCCCCCC
ATLCNPPFHDSAAAARAGSECKRRNLGLNKDDALNFGGQQQELWCEGGEVTFIKKMIEES
CEECCCCCCCCHHHHHCCHHHHHHCCCCCCCCCCCCCCCCHHEEECCCCHHHHHHHHHHH
KGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVVKKEMAQGQKQSRFIAWTFMNDEQR
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEEEECHHHH
RRFVNRQR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA