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The map label for this gene is ybiC

Identifier: 209397893

GI number: 209397893

Start: 964156

End: 965241

Strand: Direct

Name: ybiC

Synonym: ECH74115_0950

Alternate gene names: 209397893

Gene position: 964156-965241 (Clockwise)

Preceding gene: 209400968

Following gene: 209396578

Centisome position: 17.3

GC content: 55.8

Gene sequence:

>1086_bases
ATGGAAAGTGGTCATCGCTTTGATGCTCAGACGCTGCACAGCTTTATTCAGGCTGTATTTCGTCAGATGGGTAGCGAGGA
ACAAGAAGCGAAATTAGTTGCCGATCATTTAATCGCGGCAAACCTGGCAGGGCATGATTCACATGGTATTGGCATGATCC
CAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAATCGTTAAAGAGGCGGGGGCGGCG
GTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCA
GCACGGTATTGCTGCCGTGGCGCTACATAACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGG
CGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCACCGTTCCACGGTCGCGACAGCCGCTTT
GGCACGAATCCGTTCTGTGTGGTTTTCCCACGTAAAGATGATTTTCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGC
ATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGA
CGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTGGCG
GCAATGTGTGAAATTCTTGGCGGGGCGCTTTCTGGCGGTAAAACGACGCATCAGGAAACGTTACAAACCAGTCCCGATGC
CATTCTTAACTGCATGACCACTATCATCATCAACCCGGAACTGTTCGGCGCGCCGGATTGTAGCGCGCAGACCGAAGCCT
TTGCCGAGTGGGTGAAAGCCTCGCCGCATGATGACGATAAGCCGATTTTGCTCCCGGGCGAGTGGGAAGTGAACACGCGT
CGCGAACGGCAGGAGCAGGGGATTCCATTGGATGCGGGAAGCTGGCAGGCCATTTGTGATGCGGCGCGGCAGATTGGTAT
GCCGGAAGAGACGTTGCAGGCTTTCTGTCAGCAGTTAGCCAGCTAA

Upstream 100 bases:

>100_bases
CCCCACCGCAATATGAAATTCCTGCATCTTTATTGACCTTCCCGCGCCCGGCGTGCAGCATAAAAAAACAACAAACACAT
AACATAAACAGGAGTTAACC

Downstream 100 bases:

>100_bases
AAAAAAGCCCGTCCAGTGGCGGACGGGCAAACAAGGGTAACATAGGATCAATGAGGGTTAGAGCATATGCGTCTGTCGGC
AAACAGACAGGGAAATACTT

Product: hypothetical protein

Products: oxaloacetate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAA
VTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQESPLGSLLTFAEHKGYALA
AMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTR
RERQEQGIPLDAGSWQAICDAARQIGMPEETLQAFCQQLAS

Sequences:

>Translated_361_residues
MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAA
VTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQESPLGSLLTFAEHKGYALA
AMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTR
RERQEQGIPLDAGSWQAICDAARQIGMPEETLQAFCQQLAS
>Mature_361_residues
MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAA
VTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
GTNPFCVVFPRKDDFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQESPLGSLLTFAEHKGYALA
AMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTR
RERQEQGIPLDAGSWQAICDAARQIGMPEETLQAFCQQLAS

Specific function: Unknown

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family

Homologues:

Organism=Escherichia coli, GI1787020, Length=361, Percent_Identity=98.8919667590028, Blast_Score=740, Evalue=0.0,
Organism=Escherichia coli, GI1786727, Length=357, Percent_Identity=30.2521008403361, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790000, Length=331, Percent_Identity=24.4712990936556, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17507179, Length=316, Percent_Identity=29.1139240506329, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17536633, Length=348, Percent_Identity=27.2988505747126, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI45553211, Length=313, Percent_Identity=30.6709265175719, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24667912, Length=313, Percent_Identity=30.6709265175719, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24667908, Length=313, Percent_Identity=30.6709265175719, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24667904, Length=311, Percent_Identity=30.8681672025724, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24658010, Length=306, Percent_Identity=28.4313725490196, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648360, Length=331, Percent_Identity=28.0966767371601, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YBIC_ECO57 (P58409)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85589
- PIR:   G90738
- RefSeq:   NP_286565.1
- RefSeq:   NP_308906.1
- ProteinModelPortal:   P58409
- SMR:   P58409
- EnsemblBacteria:   EBESCT00000025883
- EnsemblBacteria:   EBESCT00000058001
- GeneID:   917618
- GeneID:   958033
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1022
- KEGG:   ecs:ECs0879
- GeneTree:   EBGT00050000009495
- HOGENOM:   HBG553037
- OMA:   SHGVGMI
- ProtClustDB:   PRK10098
- BioCyc:   ECOL83334:ECS0879-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003767
- PANTHER:   PTHR11091

Pfam domain/function: PF02615 Ldh_2; SSF89733 MDH_LDH

EC number: 1.1.1.37

Molecular weight: Translated: 38884; Mature: 38884

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSW
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC
SQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHH
CCCCEEECHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
IGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRFGTNPFCVVFPRKDDFPLLLD
CCHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCEEHH
YATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQESPLGSLLTFAEHKGYALA
HHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
AMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPDCSAQTEAFAEWVKA
HHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHC
SPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGSWQAICDAARQIGMPEETLQAFCQQLA
CCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
S
C
>Mature Secondary Structure
MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAGHDSHGIGMIPSYVRSW
CCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC
SQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGIEKAHQHGIAAVALHNSHH
CCCCEEECHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
IGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRFGTNPFCVVFPRKDDFPLLLD
CCHHHHHHHHHHHCCHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEECCCCCCCEEHH
YATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPAVMQESPLGSLLTFAEHKGYALA
HHHHHHHCCCCEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHH
AMCEILGGALSGGKTTHQETLQTSPDAILNCMTTIIINPELFGAPDCSAQTEAFAEWVKA
HHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHC
SPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGSWQAICDAARQIGMPEETLQAFCQQLA
CCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-malate; NAD+

Specific reaction: (S)-malate + NAD+ = oxaloacetate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796