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The map label for this gene is hsdS [H]

Identifier: 209396465

GI number: 209396465

Start: 5511477

End: 5513231

Strand: Reverse

Name: hsdS [H]

Synonym: ECH74115_5859

Alternate gene names: 209396465

Gene position: 5513231-5511477 (Counterclockwise)

Preceding gene: 209398363

Following gene: 209396668

Centisome position: 98.94

GC content: 41.71

Gene sequence:

>1755_bases
ATGAGTGTGGAAAAGCTGATCGTTGATCATATGGAAACCTGGACCTCGGCGTTGCAAACCCGTTCCACCGCCGGGCGCGG
CAGTTCCGGAAAAATTGATTTATATGGCATTAAGAAATTACGTGAGCTGATTCTGGAACTGGCTGTGCGCGGTAAACTGG
TGCCGCAGGACCCGAACGATGAACCGGCGTCGGAGCTGCTGAAGCGTATTGCGGCAGAAAAAGCAGAGCTGGTGAAGCAA
GGTAAAATTAAAAAGCAAAAACCACTGCCGGAAATTAGTGAGGAAGAGAAGCCGTTTGAATTGCCGGAGGGATGGGAGTG
GGTGCGTATTAGTGAAATTGGACATGACTGGGGACAAAAAACTCCAGATAAAGATTTTACTTACATTGATGTTGGTTCAA
TAAATAAAGAATATGGAATTATTGAAGAGCTATCTATTCTTTCAGCGAAAGATGCCCCATCGCGAGCGAGAAAAATTGTT
CAGCAAGGGACTATCATATACTCTACTGTTCGCCCGTATTTGTTAAATATAGCTATTATTGAAAACGAAATACTACCTGA
ACCGATCGCTAGTACTGCCTTCGCTATTATTCATCCATATACGGCGATGGATGCTAATTTCATTTATTATTATCTACGTT
CTCCGGTATTTGTTTGTTATGTTGAAAATTGTCAGACAGGGGTTGCCTATCCAGCAATCAATGACAAACAATTTTTTTCT
GGAATAACTCCAGTTCCTCCATCCTTGGAACAAGTTCGCATCGCAAACAAAATCAAAGAATTAATGTCCCTCTGCGACCA
ACTGGAACAGCAATCCCTGACCAGTCTGGACGCACATCAACAACTGGTTGAAACCCTGTTGGGAACGCTTACAGACAGCC
AAACCGCCGAGGAACTGGCTGAAAACTGGGCGCGTATTAGCGAGTATTTCGACACTCTATTTACCACCGAAGCCAGCGTG
GATGCGTTAAAACAGACCATTCTGCAACTGGCCGTAATGGGCAAACTTGTGCCGCAGGATCCGAATGATGAACCAGCCTC
TGAACTGCTCAAACGAATTGCGCAGGAAAAAGCTCAACTGGTGAAAGAAGGAAAAATAAAAAAACAAAAACCGTTGCCGC
CAATTAGCGATGAGGAAAAACCGTTTGAACTTCCGGAAGGGTGGGAGTGGTGTTTATTTGAAGATATTATTGATATTCAA
AGTGGTATCACTAAAGGAAGAAATTTATCAAATAGAACTTTGGTAAAAGTTCCTTATTTACGTGTTGCAAACGTCCAACG
CGGATATCTTGATCTTACGGAAATTAAACAGATTGAAATCCCTATTGAGGAAAAAGAAAAATATCAAGTAGTCAAGGGAG
ATTTATTGATAACAGAAGGCGGCGACTGGGATACAGTCGGGAGAACTACAGTATGGTGTCATGACTGGTATATAGCAAAT
CAAAACCATGTATTCAAAGGACGAAATATAGGGCAAGATGTTGATCCATATTGGTTAGAAACATATATGAATAGCCCATT
CTCAAGACAATATTTTGCTAACGCAAGTAAGCAAACCACTAATTTAGCTTCTATTAATAAAACCCAGCTCAGAGGTTGTC
CTGTTGCTATTCCTCCTAGCTCAGAAGCAAAAAAAATAATGAGTAAACTACATATTTTTTATAAACTATGTGAAGAATTA
AAGAATCATATCCAATCCGCCCAGCAAACCCAACTACACCTTGCAGATGCACTCACTGACGCGGCGGTAAACTAA

Upstream 100 bases:

>100_bases
TGTTAGCGCAGTATGCAAAACAGCAGGCGGAGATCCAGACGCTGCGTAATCAACTGCGCGATATTCTTGGCGCTGCGCTG
TCTGGCAAGGAGGTTAACTA

Downstream 100 bases:

>100_bases
TTTACCTGCCCTCGAAAAACCAATAAAGCGGCTAAATATCCGCTTTATTCACATCACCAAAATTTATATTTCACGCTAAT
TTTCATCTTATTTTCATTAA

Product: putative type I restriction-modification system, S subunit

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQ
GKIKKQKPLPEISEEEKPFELPEGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFVCYVENCQTGVAYPAINDKQFFS
GITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASV
DALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCLFEDIIDIQ
SGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQVVKGDLLITEGGDWDTVGRTTVWCHDWYIAN
QNHVFKGRNIGQDVDPYWLETYMNSPFSRQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEEL
KNHIQSAQQTQLHLADALTDAAVN

Sequences:

>Translated_584_residues
MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQ
GKIKKQKPLPEISEEEKPFELPEGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIV
QQGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFVCYVENCQTGVAYPAINDKQFFS
GITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASV
DALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCLFEDIIDIQ
SGITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQVVKGDLLITEGGDWDTVGRTTVWCHDWYIAN
QNHVFKGRNIGQDVDPYWLETYMNSPFSRQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEEL
KNHIQSAQQTQLHLADALTDAAVN
>Mature_583_residues
SVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQG
KIKKQKPLPEISEEEKPFELPEGWEWVRISEIGHDWGQKTPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIVQ
QGTIIYSTVRPYLLNIAIIENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFVCYVENCQTGVAYPAINDKQFFSG
ITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELAENWARISEYFDTLFTTEASVD
ALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCLFEDIIDIQS
GITKGRNLSNRTLVKVPYLRVANVQRGYLDLTEIKQIEIPIEEKEKYQVVKGDLLITEGGDWDTVGRTTVWCHDWYIANQ
NHVFKGRNIGQDVDPYWLETYMNSPFSRQYFANASKQTTNLASINKTQLRGCPVAIPPSSEAKKIMSKLHIFYKLCEELK
NHIQSAQQTQLHLADALTDAAVN

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 66104; Mature: 65973

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPND
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
EPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELPEGWEWVRISEIGHDWGQK
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCC
TPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIVQQGTIIYSTVRPYLLNIAII
CCCCCEEEEECCCCCCHHCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEHHHHHHEEEEE
ENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFVCYVENCQTGVAYPAINDKQFFS
CCCCCCCCHHHHHHHHHCCHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHC
GITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ENWARISEYFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VKEGKIKKQKPLPPISDEEKPFELPEGWEWCLFEDIIDIQSGITKGRNLSNRTLVKVPYL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
RVANVQRGYLDLTEIKQIEIPIEEKEKYQVVKGDLLITEGGDWDTVGRTTVWCHDWYIAN
EECCCCCCCEEHHHHHEEECCCCCCCCEEEEECCEEEECCCCCCCCCCEEEEEEEEEEEC
QNHVFKGRNIGQDVDPYWLETYMNSPFSRQYFANASKQTTNLASINKTQLRGCPVAIPPS
CCCEECCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCHHHHCCHHHHCCCCEECCCC
SEAKKIMSKLHIFYKLCEELKNHIQSAQQTQLHLADALTDAAVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELILELAVRGKLVPQDPND
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
EPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELPEGWEWVRISEIGHDWGQK
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCC
TPDKDFTYIDVGSINKEYGIIEELSILSAKDAPSRARKIVQQGTIIYSTVRPYLLNIAII
CCCCCEEEEECCCCCCHHCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEHHHHHHEEEEE
ENEILPEPIASTAFAIIHPYTAMDANFIYYYLRSPVFVCYVENCQTGVAYPAINDKQFFS
CCCCCCCCHHHHHHHHHCCHHHCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHC
GITPVPPSLEQVRIANKIKELMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQTAEELA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ENWARISEYFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKRIAQEKAQL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
VKEGKIKKQKPLPPISDEEKPFELPEGWEWCLFEDIIDIQSGITKGRNLSNRTLVKVPYL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCC
RVANVQRGYLDLTEIKQIEIPIEEKEKYQVVKGDLLITEGGDWDTVGRTTVWCHDWYIAN
EECCCCCCCEEHHHHHEEECCCCCCCCEEEEECCEEEECCCCCCCCCCEEEEEEEEEEEC
QNHVFKGRNIGQDVDPYWLETYMNSPFSRQYFANASKQTTNLASINKTQLRGCPVAIPPS
CCCEECCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCHHHHCCHHHHCCCCEECCCC
SEAKKIMSKLHIFYKLCEELKNHIQSAQQTQLHLADALTDAAVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]