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The map label for this gene is hsdM [H]

Identifier: 209398363

GI number: 209398363

Start: 5513231

End: 5514712

Strand: Reverse

Name: hsdM [H]

Synonym: ECH74115_5860

Alternate gene names: 209398363

Gene position: 5514712-5513231 (Counterclockwise)

Preceding gene: 209398938

Following gene: 209396465

Centisome position: 98.97

GC content: 50.34

Gene sequence:

>1482_bases
ATGGAATACAACATGTCTATCAGCTCAGTAATCAAATCATTACAAGATATTATGCGCAAAGATGCCGGTGTGGATGGCGA
TGCGCAGCGTCTCGGTCAGCTCTCCTGGCTGCTGTTTTTGAAAATCTTCGATGCCCAGGAAGAGGCGCTGGAACTGGAGC
AGGATAACTATCAATATCCGATCCCACAGCGTTATTTATGGCGCAGTTGGGCCGCCAACGCGCAGGGCATTACCGGTGAT
TCCCTGCTGGAATTCGTTAATGATGATCTGTTCCCGGCGTTGAAAAACCTCACTGCGCCTATCGATAAAAACCCACGCGG
CTACGTGGTGAAGCAGGCGTTCAGCGATGCCTATAACTATATGAAAAACGGTACGCTACTGCGTCAGGTGATCAATAAGC
TGAACGAAATTGACTTTACCAGCGCCAGCGAACGCCATCTGTTTGGTGATATTTACGAACAGATCCTTAAAGATCTGCAA
TCTGCGGGCAATGCGGGCGAATTCTATACTCCACGCGCCGTCACTCGCTTTATGGTGGATCGCGTTGATCCGAAACTCGG
CGAATCCATTATGGACCCGGCCTGCGGTACGGGCGGTTTTCTTGCCTGCGCATTTGATCATGTAAAGAACAAATACGTGA
AGAGCGTCGCCGATCATCAGACGCTGCAACAACAGATCCACGGTGTTGAGAAAAAACAGCTTCCGCACCTGCTGGCGACC
ACCAATATGCTGCTACACGGCATTGAAGTGCCAGTGCAAATTCGTCACGACAACACTCTGAACAAACCGCTTTCCTCCCG
GGATGAGCAACTGGATGTCATTGTTACCAACCCGCCGTTTGGTGGCACGGAAGAAGACGGTATTGAGAAGAACTTTCCGG
CAGAGATGCAAACCCGCGAAACGGCGGATTTGTTCCTGCAACTGATTGTGGAAGTACTGGCGAAAAATGGTCGCGCGGCG
GTGGTATTGCCGGATGGCACACTATTTGGCGAAGGCGTTAAAACCAAAATCAAAAAGCTACTTACCGAAGAGTGCAACCT
GCATACCATCGTGCGCTTACCGAATGGTGTGTTTAACCCCTATACCGGCATTAAAACCAACCTGTTGTTCTTTACCAAAG
GTCAGCCAACCAAAGAGATTTGGTTCTATGAGCATCCGTATCCGGCGGGCGTAAAAAACTACAGCAAAACTAAGCCGATG
AAGTTTGAAGAGTTTCAGGCGGAGATCGACTGGTGGGGTAACGAGGCCGATGGTTTTGCCAGCCGCGTAGAGAATGAGCA
GGCGTGGAAAGTCAGCATTGATGACGTGATTGCGCGTAACTTCAATCTGGATATTAAAAACCCACATCAGGCGGAAACCG
TCAGCCATGATCCGGCCGAGCTGTTAGCGCAGTATGCAAAACAGCAGGCGGAGATCCAGACGCTGCGTAATCAACTGCGC
GATATTCTTGGCGCTGCGCTGTCTGGCAAGGAGGTTAACTAA

Upstream 100 bases:

>100_bases
CGAGCTGGAAAACGAAATCTACCAATTACCGCCCCGCTCTGCTTAACAACTCTCTTTTGCGCCTTCTGCAACCATCATGT
TGCAGAAGGCCTTGTTTTTC

Downstream 100 bases:

>100_bases
TGAGTGTGGAAAAGCTGATCGTTGATCATATGGAAACCTGGACCTCGGCGTTGCAAACCCGTTCCACCGCCGGGCGCGGC
AGTTCCGGAAAAATTGATTT

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGD
SLLEFVNDDLFPALKNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQ
SAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKKQLPHLLAT
TNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAA
VVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSKTKPM
KFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPAELLAQYAKQQAEIQTLRNQLR
DILGAALSGKEVN

Sequences:

>Translated_493_residues
MEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGD
SLLEFVNDDLFPALKNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQ
SAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKKQLPHLLAT
TNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAA
VVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSKTKPM
KFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPAELLAQYAKQQAEIQTLRNQLR
DILGAALSGKEVN
>Mature_493_residues
MEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGD
SLLEFVNDDLFPALKNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQ
SAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTLQQQIHGVEKKQLPHLLAT
TNMLLHGIEVPVQIRHDNTLNKPLSSRDEQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAA
VVLPDGTLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSKTKPM
KFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETVSHDPAELLAQYAKQQAEIQTLRNQLR
DILGAALSGKEVN

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=426, Percent_Identity=28.8732394366197, Blast_Score=155, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55694; Mature: 55694

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]