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The map label for this gene is hyfE [H]

Identifier: 209396043

GI number: 209396043

Start: 3426474

End: 3427124

Strand: Direct

Name: hyfE [H]

Synonym: ECH74115_3707

Alternate gene names: 209396043

Gene position: 3426474-3427124 (Clockwise)

Preceding gene: 209396766

Following gene: 209400562

Centisome position: 61.49

GC content: 53.3

Gene sequence:

>651_bases
ATGACCGGTTCTATGATCGTAAATAATCTGGCGGGACTGATGATGCTGACATCGCTGTTTGTGATTAGCGTCAAAAGCTA
TCGCCTGTCATGCGGATTTTACGCCTGCCAGTCGCTGGTGCTGGTGTCTATTTTCGCCACCCTCTCGTGCCTGTTCGCCG
CAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTGCTGGTACCGTTAATCATGACTTACGCTGCA
CGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCT
GCTTTGCGCATTTGTCGTTCAGCCCGTGAAGCTATCGATGGCTACCGGGCTGAAACCAGCGCTGGCGGTGGCGTTAGGTC
ATTTTCTGCTTGGCCTGCTGTGCATTGTTAGCCAGCGCAATATCCTGCGGCAAATTTTTGGTTACTGCCTGATGGAAAAC
GGCTCCCATCTGGTGCTGGCGCTTCTAGCCTGGCGTGCACCGGAACTGGTGGAAATTGGTATCGCTACCGACGCCATCTT
CGCCGTCATTGTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGCACGCTGGACGTGAACAACTTGACCGCGC
TGAAGGGATAA

Upstream 100 bases:

>100_bases
CGCTGCCAGGGTCAATGCGCCTGGTGCTGATTGTACTGATTGTGATGTCGCTGATTTCCAGCGTAATCGCCGCGACCTGG
TTGCAGTAAGGAGATGATGA

Downstream 100 bases:

>100_bases
TGAGATGAGTTATTCTGTGATGTTCGCTTTACTCCTGCTCACTCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGA
AACGAGGGCACTCTGCGACT

Product: hydrogenase 4 membrane subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAA
RNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLSMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG

Sequences:

>Translated_216_residues
MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAA
RNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLSMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG
>Mature_215_residues
TGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAAR
NIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLSMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMENG
SHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788830, Length=216, Percent_Identity=99.537037037037, Blast_Score=427, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001133 [H]

Pfam domain/function: PF00420 Oxidored_q2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23352; Mature: 23221

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLSM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMENGSHLVLALLAWRAPELVEIG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
IATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSIFATLSCLFAAEQLLIW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLAALIVLLCAFVVQPVKLSM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMENGSHLVLALLAWRAPELVEIG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHH
IATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]