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The map label for this gene is hyfF [H]

Identifier: 209400562

GI number: 209400562

Start: 3427144

End: 3428709

Strand: Direct

Name: hyfF [H]

Synonym: ECH74115_3708

Alternate gene names: 209400562

Gene position: 3427144-3428709 (Clockwise)

Preceding gene: 209396043

Following gene: 209399980

Centisome position: 61.51

GC content: 54.21

Gene sequence:

>1566_bases
ATGTTCGCTTTACTCCTGCTCACTCCGCTGCTTTTTTCGCTGCTCTGTTTTGCCTGCCGGAAACGAGGGCACTCTGCGAC
TCGCACGGTGACAGTATTACATAGCTTAGGGATCACACTGCTGCTGATTCTGGCACTCTGGGTGGTCCAAACTGCCGCTG
ATGCAGGAGAAATATTCGCTGCGGGACTGTGGCTTCATATTGATGGTCTGGGCGGTTTGTTCCTCGCCATTCTTGGTGTG
ATTGGCTTTCTCACCGGTGTTTACTCGATTGGCTACATGCGTCATGAAGTTGAGCACGGCGAGCTTTCACCCGTTACGCT
GTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGT
GGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCATCGCTGGAAGCAGCA
TGGAAGTACATCATTATTTGTACTGTTGGTGTCGCTTTTGGTCTGTTCGGTACCGTGCTAGTATACGCCAACGCCGCCAG
CGTTATGCCGCAGGCAGAAATGGCGATATTCTGGAGCGAGGTTCTTAAGCAATCGTCCTTGCTTGATCCAACATTAATGC
TGTTGGCCTTTGTGTTTGTGCTAATTGGCTTTGGTACCAAAACCGGGCTATTTCCCATGCACGCCTGGCTGCCGGATGCT
CACAGTGAAGCGCCGAGTCCGGTCAGCGCCCTACTCTCCGCCGTATTGCTGAACTGCGCGCTGTTGGTGCTGATTCGCTA
TTACATCATTATTTGCCAGGCCATCGGCAGCGATTTCCCCAACCGGTTGTTGCTCATCTTCGGCATGTTGTCGGTTGCCG
TGGCGGCATTTTTCATTCTGGTACAGCGGGACATTAAGCGTCTGCTGGCGTACTCCAGCGTGGAGAACATGGGACTGGTC
GCGGTGGCGCTAGGCATTGGCGGGCCGCTGGGAATTTTTGCCGCGCTGCTGCACACCTTAAACCACAGTCTGGCAAAAAC
GCTGCTGTTCTGCGGTTCCGGCAATGTACTGCTCAAGTACGGCACGCGCGATCTCAACGTCGTCTGTGGGATGCTCAAAA
TCATGCCATTTACCGCCGTGCTGTTTGGCGGCGGTGCGCTGGCGCTGGCAGGGATGCCGCCCTTCAACATTTTTCTTAGC
GAATTTATGACCGTTACCGCCGGACTGGCACGTAATCACCTGCTGATTATCGTCCTGCTGTTATTGCTGTTAACGCTGGT
GCTGGCGGGCCTGGTACGGATGGCTGCGCGGGTGTTAATGGCGAAACCGCCGCAGGCCGTTAACCGGGGTGATCTCGGCT
GGTTGACCACCTCGCCAATGGTGATTCTGCTGGTCATGATGCTGGCGATGGGAACGCATATTCCACAACCTGTCATCAGG
ATCCTGGCGGGCGCTTCCACTATAGTCCTCTCAGGGACGCACGACCTGCCTGCACAACGTAGCACCTGGCATGATTTTTT
GCCTTCAGGCACCGCATCTGTTTCGGAGAAACACAGTGAACGTTAA

Upstream 100 bases:

>100_bases
GTGATGGTGTTACTGGCAAGAAAAATATGGCGTACCCACGGCACGCTGGACGTGAACAACTTGACCGCGCTGAAGGGATA
ATGAGATGAGTTATTCTGTG

Downstream 100 bases:

>100_bases
TTCATCGTCAAATCGTGGCGAAGCGATTCTCGCCGCCCTGAAAACGCAGTTCCCCGGCGCGGTGCTGGATGAAGAGCGAC
AAACGCCTGAACAGGTCACC

Product: hydrogenase 4 subunit F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGV
IGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAA
WKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDA
HSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLV
AVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLS
EFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIR
ILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Sequences:

>Translated_521_residues
MFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGV
IGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAA
WKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDA
HSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLV
AVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLS
EFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIR
ILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
>Mature_521_residues
MFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGV
IGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAA
WKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDA
HSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLV
AVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLS
EFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIR
ILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=383, Percent_Identity=27.154046997389, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI251831116, Length=184, Percent_Identity=31.5217391304348, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788831, Length=521, Percent_Identity=98.4644913627639, Blast_Score=1018, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=357, Percent_Identity=30.5322128851541, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1788613, Length=432, Percent_Identity=28.4722222222222, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=29.3302540415704, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788614, Length=439, Percent_Identity=28.0182232346241, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI2367154, Length=353, Percent_Identity=29.1784702549575, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693160, Length=205, Percent_Identity=33.1707317073171, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56071; Mature: 56071

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFA
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFT
HHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
MLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGLFGTVL
HHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCC
HSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKY
HHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLL
CCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
ILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure
MFALLLLTPLLFSLLCFACRKRGHSATRTVTVLHSLGITLLLILALWVVQTAADAGEIFA
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
AGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEVEHGELSPVTLCDYYGFFHLFLFT
HHHEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
MLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSSLEAAWKYIIICTVGVAFGLFGTVL
HHHHHHCCCEEEEEEEHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VYANAASVMPQAEMAIFWSEVLKQSSLLDPTLMLLAFVFVLIGFGTKTGLFPMHAWLPDA
HHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCC
HSEAPSPVSALLSAVLLNCALLVLIRYYIIICQAIGSDFPNRLLLIFGMLSVAVAAFFIL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
VQRDIKRLLAYSSVENMGLVAVALGIGGPLGIFAALLHTLNHSLAKTLLFCGSGNVLLKY
HHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
GTRDLNVVCGMLKIMPFTAVLFGGGALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLL
CCCHHHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLLTLVLAGLVRMAARVLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHH
ILAGASTIVLSGTHDLPAQRSTWHDFLPSGTASVSEKHSER
HHHCCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]