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The map label for this gene is pgaC

Identifier: 209395977

GI number: 209395977

Start: 1271750

End: 1272988

Strand: Reverse

Name: pgaC

Synonym: ECH74115_1264

Alternate gene names: 209395977

Gene position: 1272988-1271750 (Counterclockwise)

Preceding gene: 209397733

Following gene: 209396546

Centisome position: 22.85

GC content: 47.3

Gene sequence:

>1239_bases
ATGATGAGGTTCGTTTTCTTCTGGCCGTTTTTTATGTCCATTATGTGGATTGTTGGCGGCGTCTATTTCTGGGTCTATCG
TGAACGCCGCTGGCCGTGGGGAGAAAACGCACCAGCTCCCCAGTTGAAAGATAATCCGTCTATCTCCATTATCATTCCCT
GTTTTAATGAGGAGAAAAACGTTGAGGAAACCATACACGCCGCTTTAGCACAGCGTTATGAGAACATTGAAGTTATTGCC
GTAAATGACGGTTCAACAGATAAAACCCGTGCCATCCTGGATCGCATGGCTGCACAAATTCCCCATTTGCGGGTCATTCA
TCTGGCGCAAAACCAGGGGAAAGCCATTGCGCTTAAAACCGGAGCTGCCGCGGCGAAAAGTGAATATCTGGTGTGCATTG
ATGGCGATGCGTTATTAGACCGCGATGCGGCGGCATATATTGTGGAACCGATGTTGTACAACCCGCGTGTGGGTGCCGTA
ACCGGTAATCCTCGTATTCGAACACGTTCTACCCTGGTGGGTAAAATTCAGGTTGGCGAGTATTCCTCAATTATTGGTTT
GATCAAGCGAACCCAGCGTATCTATGGAAACGTATTTACCGTTTCCGGTGTTATTGCCGCATTTCGTCGCAGCGCCCTGG
CAGAAGTGGGTTACTGGAGTGACGATATGATCACCGAAGATATTGATATTAGCTGGAAGCTGCAGTTGAATCAGTGGACG
ATTTTTTACGAGCCACGGGCACTGTGCTGGATATTAATGCCTGAAACGTTAAAAGGGCTGTGGAAACAGCGCCTGCGCTG
GGCTCAGGGCGGTGCAGAAGTATTCCTCAAAAATATGACAAGGTTGTGGCGCAAAGAAAACTTTCGAATGTGGCCGCTGT
TTTTTGAATACAGCCTGACGACAATATGGGCCTTCACCTGCCTGGTCGGTTTCATTATTTACGCAGTCCAACTTGCCGGT
GTACCGTTAAATATTGAATTGACACATATCGCTGCGACACATACTGCCGGAATATTATTGTGTACGTTATGTTTACTGCA
ATTTATTGTCAGCCTGATGATCGAGAATCGCTATGAGCATAATCTGACTTCATCGCTTTTCTGGATTATTTGGTTCCCGG
TTATTTTCTGGATGCTGAGCCTGGCAACGACATTGGTATCATTTACACGAGTCATGTTGATGCCTAAAAAGCAACGCGCC
CGTTGGGTAAGTCCCGATCGCGGGATTCTGAGAGGTTAA

Upstream 100 bases:

>100_bases
TGGTATCCGAAAAATGATTAATCGCATCGTATCGTTTTTTATATTATGTCTGGTGTTATGCATACCCCTATGCGTAGCGT
ACTTTCACTCTGGTGAACTG

Downstream 100 bases:

>100_bases
TATGAACAATTTAATTATTACGACCCGACAATCACCAGTACGTTTACTGGTTGATTATGTTGCCACAACCATCTTGTGGA
CATTATTTGCGTTGTTCATA

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MMRFVFFWPFFMSIMWIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIA
VNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAV
TGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWT
IFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAFTCLVGFIIYAVQLAG
VPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRA
RWVSPDRGILRG

Sequences:

>Translated_412_residues
MMRFVFFWPFFMSIMWIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIA
VNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAV
TGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWT
IFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAFTCLVGFIIYAVQLAG
VPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRA
RWVSPDRGILRG
>Mature_412_residues
MMRFVFFWPFFMSIMWIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIA
VNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAV
TGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWT
IFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLTTIWAFTCLVGFIIYAVQLAG
VPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRA
RWVSPDRGILRG

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family

Homologues:

Organism=Escherichia coli, GI1787259, Length=412, Percent_Identity=99.5145631067961, Blast_Score=844, Evalue=0.0,
Organism=Escherichia coli, GI1786560, Length=219, Percent_Identity=29.2237442922374, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PGAC_ECO57 (Q8XAR5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85647
- PIR:   D90787
- RefSeq:   NP_287029.1
- RefSeq:   NP_309295.1
- ProteinModelPortal:   Q8XAR5
- SMR:   Q8XAR5
- EnsemblBacteria:   EBESCT00000026600
- EnsemblBacteria:   EBESCT00000057554
- GeneID:   912769
- GeneID:   959160
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1524
- KEGG:   ecs:ECs1268
- GeneTree:   EBGT00050000008880
- HOGENOM:   HBG514801
- OMA:   FGRIFTV
- ProtClustDB:   PRK11204
- BioCyc:   ECOL83334:ECS1268-MONOMER
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: NA

Molecular weight: Translated: 47472; Mature: 47472

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x249f22f8)-; HASH(0x268ee3ac)-; HASH(0x268e4fe8)-; HASH(0x2567928c)-; HASH(0x25bb3784)-;

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRFVFFWPFFMSIMWIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKN
CCEEEEHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
VEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKT
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEC
GAAAAKSEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGE
CCCCCCCCEEEEECCCHHHCCCCHHHEECCCEECCEEEEEECCCCEEECCEEEEEEEECC
YSSIIGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEEEEEEE
IFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLT
EEECCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHH
TIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEH
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
NLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCH
>Mature Secondary Structure
MMRFVFFWPFFMSIMWIVGGVYFWVYRERRWPWGENAPAPQLKDNPSISIIIPCFNEEKN
CCEEEEHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCC
VEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKT
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEC
GAAAAKSEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGE
CCCCCCCCEEEEECCCHHHCCCCHHHEECCCEECCEEEEEECCCCEEECCEEEEEEEECC
YSSIIGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWT
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCEEEEEEEEEEE
IFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYSLT
EEECCCEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEHHHHH
TIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEH
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHC
NLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796