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The map label for this gene is pgaB

Identifier: 209397733

GI number: 209397733

Start: 1273068

End: 1275086

Strand: Reverse

Name: pgaB

Synonym: ECH74115_1265

Alternate gene names: 209397733

Gene position: 1275086-1273068 (Counterclockwise)

Preceding gene: 209397134

Following gene: 209395977

Centisome position: 22.88

GC content: 44.33

Gene sequence:

>2019_bases
ATGTTACGTAATGGAAATAAATATCTCCTGATGCTGGTGAGTATAATTATGCTCACCGCGTGCATTAGCCAGTCAAGAAC
ATCATTTATACCGCCACAGGATCGCAAATCTTTACTCGCCGAGCAACCGTGGCCGCATAATGGTTTTGTAGCGATTTCAT
GGCATAACGTTGAAGACGAAGCTGCCGACCAGCGTTTTATGTCAGTGCGGACATCAGCACTGCGTGAACAATTTGCCTGG
CTGCGCGAGAACGGTTATCAACCGGTCAGTATTGCTCAAATTCGTGAAGCACATCGAGGAGGAAAACCGCTACCGGAAAA
AGCTGTAGTGCTGACTTTTGATGACGGCTACCAGAGTTTTTATACCCGCGTCTTCCCAATTCTTCAGGCCTTCCAGTGGC
CTGCTGTATGGGCCCCCGTCGGCAGTTGGGTCGATACGCCAGCGGATAAACAAGTAAAATTTGGCGATGAGTTGGTCGAT
CGAGAATATTTTGCCACGTGGCAACAAGTGCGAGAAGTTGCGCGTTCCCGGCTCGTTGAGCTCGCTTCTCATACATGGAA
TTCTCACTACGGTATTCAGGCTAATGCCACCGGCAGCTTATTGCCTGTATATGTAAATCGTGCATATTTTACTGACCACG
CACGGTATGAAACCGCAGCAGAATACCGGGAAAGAATTCGTCTGGATGCTGTAAAAATGACGGAATACCTGCGTACAAAG
GTTGAGGTAAATCCACACGTTTTTATTTGGCCTTATGGCGAAGCGAATGGCATAGCGATAGAGGAATTAAAAAAACTCGG
TTATGACATGTTCTTCACCCTTGAATCAGGTTTGGCAAATGCGTCGCAATTGGATTCCATTCCGCGGGTATTAATCGCCA
ATAATCCCTCATTAAAAGAGTTTGCCCAGCAAATTATTACCGTACAGGAAAAATCACCACAACGGATAATGCATATCGAT
CTTGATTACGTTTATGACGAAAACCTCCAGCAAATGGATCGCAATATTGATGTGCTAATTCAGCGGGTGAAAGATATGCA
AATATCAACCGTGTATTTGCAGGCATTTGCTGATCCCGATGGTGATGGGCTGGTCAAAGAGGTCTGGTTTCCAAATCGTT
TGCTACCAATGAAAGCAGATATTTTTAGTCGGGTTGCCTGGCAATTACGTACCCGCTCAGGTGTAAACATCTATGCGTGG
ATGCCGGTATTAAGCTGGGATTTAGATCCCACATTAACGCGAGTAAAATACTTACCAACAGGGGAGAAAAAAGCACAAAT
TCATCCTGAACAATATCACCGTCTCTCTCCTTTCGATGACAGAGTCAGAGCACAAGTTGGCATGTTATATGAAGATCTTG
CCGGACATGCTGCTTTTGATGGCATATTGTTCCACGATGATGCTTTGCTTTCAGATTATGAAGATGCCAGTGCACCGGCT
ATCACGGCTTATCAGCAAGCAGGCTTTAGCGGGAGTCTGAGCGAAATTCGACAAAACCCGGAGCAATTTAAACAGTGGGC
CCGCTTTAAAAGTCGTGCGTTAACTGACTTCACTTTAGAACTTAGTGCGCGCGTAAAAGCCATTCGCGGTCCACATATTA
AAACTGCACGAAATATTTTTGCACTTCCGGTAATACAACCTGAAAGTGAAGCCTGGTTTGCACAGAATTATGCTGATTTC
CTAAAAAGCTATGACTGGACCGCTATTATGGCTATGCCTTATCTGGAAGGTGTCGCAGAAAAATCGGCTGACCAATGGTT
AATACAATTGACCAATCAAATTAAAAACATCCCTCAGGCTAAAGACAAATCTATTTTAGAATTACAGGCACAAAACTGGC
AGAAAAATGGTCAGCATCAGGCTATTTCTTCGCAACAACTCGCTCACTGGATGAGCCTATTACAACTGAATGGAGTGAAA
AACTATGGTTATTATCCCGACAATTTTCTGCATAACCAACCTGAAATAGACCTTATTCGTCCTGAGTTTTCAACAGCCTG
GTATCCGAAAAATGATTAA

Upstream 100 bases:

>100_bases
CTGGCGCAACGCTACGCTGGGAAAAACGACCTTATGACGGTGACAGAGAACACAACTTATACGTTGAATTCGATATGACA
TTCAGATTTTAAGGATAAAT

Downstream 100 bases:

>100_bases
TCGCATCGTATCGTTTTTTATATTATGTCTGGTGTTATGCATACCCCTATGCGTAGCGTACTTTCACTCTGGTGAACTGA
TGATGAGGTTCGTTTTCTTC

Product: outer membrane N-deacetylase

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPWPHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW
LRENGYQPVSIAQIREAHRGGKPLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK
VEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHID
LDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAW
MPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA
ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADF
LKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVK
NYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND

Sequences:

>Translated_672_residues
MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPWPHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW
LRENGYQPVSIAQIREAHRGGKPLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK
VEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHID
LDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAW
MPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA
ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADF
LKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVK
NYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND
>Mature_672_residues
MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPWPHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAW
LRENGYQPVSIAQIREAHRGGKPLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK
VEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHID
LDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAW
MPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA
ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADF
LKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVK
NYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family

Homologues:

Organism=Escherichia coli, GI1787260, Length=672, Percent_Identity=99.7023809523809, Blast_Score=1389, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PGAB_ECO57 (Q8XAR3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85647
- PIR:   E90787
- RefSeq:   NP_287030.1
- RefSeq:   NP_309296.1
- ProteinModelPortal:   Q8XAR3
- EnsemblBacteria:   EBESCT00000027800
- EnsemblBacteria:   EBESCT00000055830
- GeneID:   912831
- GeneID:   959161
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z1525
- KEGG:   ecs:ECs1269
- GeneTree:   EBGT00050000010369
- HOGENOM:   HBG296747
- OMA:   LDYVYDP
- ProtClustDB:   PRK14582
- BioCyc:   ECOL83334:ECS1269-MONOMER
- InterPro:   IPR011330
- InterPro:   IPR002509
- Gene3D:   G3DSA:3.20.20.370

Pfam domain/function: PF01522 Polysacc_deac_1; SSF88713 Glyco_hydro/deAcase_b/a-brl

EC number: NA

Molecular weight: Translated: 77427; Mature: 77427

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPWPHNGFVAISWHNVEDE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHH
AADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGKPLPEKAVVLTFDDGYQSF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHH
YTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVDREYFATWQQVREVARSRLVE
HHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK
HHHHCCCCCCCEEECCCCCEEEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKE
EECCCEEEEEECCCCCCCCHHHHHHCCCEEEEEEHHCCCCHHHHHCCCEEEEECCCCHHH
FAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPD
HHHHHHHHCCCCCCEEEEEEEHHHHCCCHHHHHCCHHHHHHHHHCCEEEEEEEEEECCCC
GDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAWMPVLSWDLDPTLTRVKYLPT
CCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHEEECCC
GEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCEEEECCHHHCCCCCCCCCE
ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIF
EHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHHCCCCCHHHCCEE
ALPVIQPESEAWFAQNYADFLKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQA
EEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIR
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEC
PEFSTAWYPKND
CCCCCCCCCCCC
>Mature Secondary Structure
MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRKSLLAEQPWPHNGFVAISWHNVEDE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCHH
AADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGKPLPEKAVVLTFDDGYQSF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHH
YTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVDREYFATWQQVREVARSRLVE
HHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYETAAEYRERIRLDAVKMTEYLRTK
HHHHCCCCCCCEEECCCCCEEEEEEEEEHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEVNPHVFIWPYGEANGIAIEELKKLGYDMFFTLESGLANASQLDSIPRVLIANNPSLKE
EECCCEEEEEECCCCCCCCHHHHHHCCCEEEEEEHHCCCCHHHHHCCCEEEEECCCCHHH
FAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDRNIDVLIQRVKDMQISTVYLQAFADPD
HHHHHHHHCCCCCCEEEEEEEHHHHCCCHHHHHCCHHHHHHHHHCCEEEEEEEEEECCCC
GDGLVKEVWFPNRLLPMKADIFSRVAWQLRTRSGVNIYAWMPVLSWDLDPTLTRVKYLPT
CCCCHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHEEECCC
GEKKAQIHPEQYHRLSPFDDRVRAQVGMLYEDLAGHAAFDGILFHDDALLSDYEDASAPA
CCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHCCEEEECCHHHCCCCCCCCCE
ITAYQQAGFSGSLSEIRQNPEQFKQWARFKSRALTDFTLELSARVKAIRGPHIKTARNIF
EHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHCCHHEEEEHHHHHHHHCCCCCHHHCCEE
ALPVIQPESEAWFAQNYADFLKSYDWTAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQA
EEEEECCCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KDKSILELQAQNWQKNGQHQAISSQQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIR
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEC
PEFSTAWYPKND
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796