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The map label for this gene is atpF

Identifier: 209696447

GI number: 209696447

Start: 3318604

End: 3319074

Strand: Reverse

Name: atpF

Synonym: VSAL_I3063

Alternate gene names: 209696447

Gene position: 3319074-3318604 (Counterclockwise)

Preceding gene: 209696448

Following gene: 209696446

Centisome position: 99.82

GC content: 42.68

Gene sequence:

>471_bases
TTGAATATGAACGCAACTCTGCTTGGTCAAGCAATCGCTTTTGCTCTGTTTGTTTGGTTCTGCATGAAATATGTATGGCC
ACCAATCATGGAAGCGATAGAGGAACGTCAGAAGAAAATTGCTGACGGTTTATCTGCCGCTGAACGCGCAGCTAAAGACC
TTGACTTGGCACAAGCGAATGCTTCTGATCAATTGAAAGAAGCAAAGCGCGCTGCAACTGAGATCATCGAACAAGCAAAT
AAGCGTAAAGGTCAAATTTTAGATGAAGCTCGTGAAGAAGCTCTGACAGAACGTCAGAATATTCTTACTCAAGGTGAAGC
TGAACTTGAAACTGAACGCAACCGTGCTCGCGATGAACTGCGTAAACAAGTTGCAACTCTGGCTGTGATTGGTGCTGAGA
AAATCTTGGAGCGTTCTATTAATTTAGAAGCGCAGAAAGATATTCTTGATAACATCACTGCGAAATTGTAA

Upstream 100 bases:

>100_bases
TACTATTCACATTTGCTAACCCATTTGTTGGTCAACTAGCGGGCTAATTGCCACAGCTAATTGAATTTTTGTAGCTGATT
CTTAACGAGGGGTAGCTGTT

Downstream 100 bases:

>100_bases
GTAAAAGGGGCTAAGATATGTCGACAATGACAACCATCGCACGCCCCTACGCGAAAGCAGCTTTTGACTTTGCGGTCGAA
AAGAACGAATTGAACCAATG

Product: F0F1 ATP synthase subunit B

Products: ADP; phosphate; H+

Alternate protein names: ATP synthase F(0) sector subunit b; ATPase subunit I; F-type ATPase subunit b; F-ATPase subunit b

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MNMNATLLGQAIAFALFVWFCMKYVWPPIMEAIEERQKKIADGLSAAERAAKDLDLAQANASDQLKEAKRAATEIIEQAN
KRKGQILDEAREEALTERQNILTQGEAELETERNRARDELRKQVATLAVIGAEKILERSINLEAQKDILDNITAKL

Sequences:

>Translated_156_residues
MNMNATLLGQAIAFALFVWFCMKYVWPPIMEAIEERQKKIADGLSAAERAAKDLDLAQANASDQLKEAKRAATEIIEQAN
KRKGQILDEAREEALTERQNILTQGEAELETERNRARDELRKQVATLAVIGAEKILERSINLEAQKDILDNITAKL
>Mature_156_residues
MNMNATLLGQAIAFALFVWFCMKYVWPPIMEAIEERQKKIADGLSAAERAAKDLDLAQANASDQLKEAKRAATEIIEQAN
KRKGQILDEAREEALTERQNILTQGEAELETERNRARDELRKQVATLAVIGAEKILERSINLEAQKDILDNITAKL

Specific function: Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0)

COG id: COG0711

COG function: function code C; F0F1-type ATP synthase, subunit b

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase B chain family

Homologues:

Organism=Escherichia coli, GI1790174, Length=156, Percent_Identity=68.5897435897436, Blast_Score=214, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ATPF_ALISL (B6EHU1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264378.1
- GeneID:   6988284
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I3063
- HOGENOM:   HBG617328
- OMA:   NPGLIFW
- ProtClustDB:   PRK05759
- HAMAP:   MF_01398
- InterPro:   IPR002146
- InterPro:   IPR005864
- TIGRFAMs:   TIGR01144

Pfam domain/function: PF00430 ATP-synt_B

EC number: 3.6.3.14

Molecular weight: Translated: 17535; Mature: 17535

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x23166d0c)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMNATLLGQAIAFALFVWFCMKYVWPPIMEAIEERQKKIADGLSAAERAAKDLDLAQAN
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASDQLKEAKRAATEIIEQANKRKGQILDEAREEALTERQNILTQGEAELETERNRARDEL
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RKQVATLAVIGAEKILERSINLEAQKDILDNITAKL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCH
>Mature Secondary Structure
MNMNATLLGQAIAFALFVWFCMKYVWPPIMEAIEERQKKIADGLSAAERAAKDLDLAQAN
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ASDQLKEAKRAATEIIEQANKRKGQILDEAREEALTERQNILTQGEAELETERNRARDEL
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RKQVATLAVIGAEKILERSINLEAQKDILDNITAKL
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: Borate; diphosphate; HCO3- [C]

Metal ions: Co2+; Fe2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; H+

Specific reaction: ATP + H2O + H+(in) = ADP + phosphate + H+(out)

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Ca2+; CN-; Efrapeptin; Ethidiumbromide; Guanidines analogs; Oligomycin; Quercetin; Trialkyl tin derivatives; Venturicidin [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA