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The map label for this gene is rmlB [H]

Identifier: 209696410

GI number: 209696410

Start: 3269745

End: 3270860

Strand: Direct

Name: rmlB [H]

Synonym: VSAL_I3018

Alternate gene names: 209696410

Gene position: 3269745-3270860 (Clockwise)

Preceding gene: 209696409

Following gene: 209696411

Centisome position: 98.33

GC content: 39.87

Gene sequence:

>1116_bases
ATGAAAATTTTAGTCACAGGTGGAGCTGGTTTTATTGGCTCAGCAGTAGTGCGTCACATTATCAATAATACACAAGATAG
TGTTATCAATGTCGATAAATTAACTTACGCTGGTAACCTAGAATCGTTAGCGAGTATTGAGTCAAATGAACGTTATGTTT
TCGAGCAAGTTGATATATGTGACCGTGCCGAATTAGACAGAGTTTTCGCACAATGCAAGCCTGATGCTGTTATGCATTTA
GCAGCAGAGTCACATGTTGATCGCTCAATCACGGGCCCGGCAGATTTCATCCAGACTAATATCGTGGGCACCTATACACT
TCTTGAAGCGACAAGAGCGTATTGGAACACATTATCTGAAGATGCTAAGAAAGCTTTTCGTTTTCATCATATCTCTACTG
ACGAAGTCTATGGCGATTTACCGCATCCAGACGATATTGAAGATGGTACAGAGCCTTCAGCGAATAAAGAGTTACCGTTA
TTTACCGAAACAACATCATACGAGCCATCAAGCCCATATTCTGCATCTAAGGCTAGTTCTGATCATTTAGTTCGTGCTTG
GTTAAGAACCTATGGGTTACCAACAATAGTAACCAACTGTTCAAATAACTATGGTCCATATCACTTCCCTGAAAAGCTGA
TCCCACTGGTTATTTTAAATGCATTAGAAGGAAAACCTTTACCTATTTACGGTAAAGGTGATCAAATTCGCGATTGGTTG
TTTGTTGAGGACCACGCACGTGCGCTCTATAAAGTTGTGACTGAAGGTAAAGTCGGTGAAACCTACAACATCGGTGGTCA
TAACGAAAAGCAAAACTTAGAAGTTGTTCAGACGATTTGCGCAATTTTAGATTCTTTAGTGCCAAAAGAATCTAAGTACC
AAGAACAAATCACTTATGTAACAGATAGGCCAGGCCATGACCGACGTTATGCAATTGATTCAAGTAAAATGCAGCGAGAA
CTCAGTTGGACACCACTAGAAACTTTTGAAACAGGTCTTAAAAAGACGATTGAATGGTATTTAAATAACCAAGAATGGTG
TCAACATGTACAAGATGGCAGCTATCAACGTGAGCGCCTAGGTGTAGAGTCAGTAGAATCAAAGGAAAGTAAATAA

Upstream 100 bases:

>100_bases
CTTGGTTCTATAGGCTGAGATTGGGATGTGTGATTTTTCAATTCACAACGTCTAAATTGAACTTGTTTTTAGGCAAGTAA
TACATCTTGGAAGCATAAAA

Downstream 100 bases:

>100_bases
TGAAAGGGATTGTTCTCGCTGGTGGTTCAGGTACACGTCTGTACCCATTAACTCGTGGTGTATCTAAACAACTATTGCCT
ATCTACGATAAGCCAATGGT

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDICDRAELDRVFAQCKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSEDAKKAFRFHHISTDEVYGDLPHPDDIEDGTEPSANKELPL
FTETTSYEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWL
FVEDHARALYKVVTEGKVGETYNIGGHNEKQNLEVVQTICAILDSLVPKESKYQEQITYVTDRPGHDRRYAIDSSKMQRE
LSWTPLETFETGLKKTIEWYLNNQEWCQHVQDGSYQRERLGVESVESKESK

Sequences:

>Translated_371_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDICDRAELDRVFAQCKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSEDAKKAFRFHHISTDEVYGDLPHPDDIEDGTEPSANKELPL
FTETTSYEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWL
FVEDHARALYKVVTEGKVGETYNIGGHNEKQNLEVVQTICAILDSLVPKESKYQEQITYVTDRPGHDRRYAIDSSKMQRE
LSWTPLETFETGLKKTIEWYLNNQEWCQHVQDGSYQRERLGVESVESKESK
>Mature_371_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDICDRAELDRVFAQCKPDAVMHL
AAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSEDAKKAFRFHHISTDEVYGDLPHPDDIEDGTEPSANKELPL
FTETTSYEPSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWL
FVEDHARALYKVVTEGKVGETYNIGGHNEKQNLEVVQTICAILDSLVPKESKYQEQITYVTDRPGHDRRYAIDSSKMQRE
LSWTPLETFETGLKKTIEWYLNNQEWCQHVQDGSYQRERLGVESVESKESK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=34.6938775510204, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI42516563, Length=350, Percent_Identity=23.4285714285714, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI56237023, Length=354, Percent_Identity=27.683615819209, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=354, Percent_Identity=27.683615819209, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=354, Percent_Identity=27.683615819209, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=77.247191011236, Blast_Score=590, Evalue=1e-170,
Organism=Escherichia coli, GI48994969, Length=366, Percent_Identity=71.0382513661202, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1786974, Length=365, Percent_Identity=26.027397260274, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788366, Length=313, Percent_Identity=24.6006389776358, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=36.950146627566, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=340, Percent_Identity=32.6470588235294, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71982035, Length=370, Percent_Identity=28.3783783783784, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=372, Percent_Identity=28.2258064516129, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=344, Percent_Identity=23.8372093023256, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=375, Percent_Identity=27.2, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19923002, Length=371, Percent_Identity=26.4150943396226, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356223, Length=360, Percent_Identity=25.8333333333333, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 41926; Mature: 41926

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHCCCCCCEEEEHHCCH
DRAELDRVFAQCKPDAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSE
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
DAKKAFRFHHISTDEVYGDLPHPDDIEDGTEPSANKELPLFTETTSYEPSSPYSASKASS
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCH
DHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWL
HHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHEE
FVEDHARALYKVVTEGKVGETYNIGGHNEKQNLEVVQTICAILDSLVPKESKYQEQITYV
EEHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHEEE
TDRPGHDRRYAIDSSKMQRELSWTPLETFETGLKKTIEWYLNNQEWCQHVQDGSYQRERL
ECCCCCCCEEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHC
GVESVESKESK
CHHHHHHCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVINVDKLTYAGNLESLASIESNERYVFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEHHHEEECCCHHHHHHCCCCCCEEEEHHCCH
DRAELDRVFAQCKPDAVMHLAAESHVDRSITGPADFIQTNIVGTYTLLEATRAYWNTLSE
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
DAKKAFRFHHISTDEVYGDLPHPDDIEDGTEPSANKELPLFTETTSYEPSSPYSASKASS
HHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCH
DHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKPLPIYGKGDQIRDWL
HHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHEE
FVEDHARALYKVVTEGKVGETYNIGGHNEKQNLEVVQTICAILDSLVPKESKYQEQITYV
EEHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHEEE
TDRPGHDRRYAIDSSKMQRELSWTPLETFETGLKKTIEWYLNNQEWCQHVQDGSYQRERL
ECCCCCCCEEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHC
GVESVESKESK
CHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]